PDE5A (NM_033430) Human Untagged Clone

CAT#: SC120663

PDE5A (untagged)-Human phosphodiesterase 5A, cGMP-specific (PDE5A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE5A
• Synonyms: CGB-PDE; CN5A; PDE5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_033430, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTGCCCTTTGGAGACAAAACAAGAGAAATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACCATCC
  CTGTGTGCAAGGAAGGTATCAGAGGCCACACCGAATCTTGCTCTTGTCCCTTGCAGCAGAGTCCTCGTGC
  AGATAACAGTGCCCCTGGAACACCAACCAGGAAAATCTCTGCCTCTGAATTTGACCGGCCTCTTAGACCC
  ATTGTTGTCAAGGATTCTGAGGGAACTGTGAGCTTCCTCTCTGACTCAGAAAAGAAGGAACAGATGCCTC
  TAACCCCTCCAAGGTTTGATCATGATGAAGGGGACCAGTGCTCAAGACTCTTGGAATTAGTGAAGGATAT
  TTCTAGTCATTTGGATGTCACAGCCTTATGTCACAAAATTTTCTTGCATATCCATGGACTGATATCTGCT
  GACCGCTATTCCCTGTTCCTTGTCTGTGAAGACAGCTCCAATGACAAGTTTCTTATCAGCCGCCTCTTTG
  ATGTTGCTGAAGGTTCAACACTGGAAGAAGTTTCAAATAACTGTATCCGCTTAGAATGGAACAAAGGCAT
  TGTGGGACATGTGGCAGCGCTTGGTGAGCCCTTGAACATCAAAGATGCATATGAGGATCCTCGGTTCAAT
  GCAGAAGTTGACCAAATTACAGGCTACAAGACACAAAGCATTCTTTGTATGCCAATTAAGAATCATAGGG
  AAGAGGTTGTTGGTGTAGCCCAGGCCATCAACAAGAAATCAGGAAACGGTGGGACATTTACTGAAAAAGA
  TGAAAAGGACTTTGCTGCTTATTTGGCATTTTGTGGTATTGTTCTTCATAATGCTCAGCTCTATGAGACT
  TCACTGCTGGAGAACAAGAGAAATCAGGTGCTGCTTGACCTTGCTAGTTTAATTTTTGAAGAACAACAAT
  CATTAGAAGTAATTTTGAAGAAAATAGCTGCCACTATTATCTCTTTCATGCAAGTGCAGAAATGCACCAT
  TTTCATAGTGGATGAAGATTGCTCCGATTCTTTTTCTAGTGTGTTTCACATGGAGTGTGAGGAATTAGAA
  AAATCATCTGATACATTAACAAGGGAACATGATGCAAACAAAATCAATTACATGTATGCTCAGTATGTCA
  AAAATACTATGGAACCACTTAATATCCCAGATGTCAGTAAGGATAAAAGATTTCCCTGGACAACTGAAAA
  TACAGGAAATGTAAACCAGCAGTGCATTAGAAGTTTGCTTTGTACACCTATAAAAAATGGAAAGAAGAAT
  AAAGTTATAGGGGTTTGCCAACTTGTTAATAAGATGGAGGAGAATACTGGCAAGGTTAAGCCTTTCAACC
  GAAATGACGAACAGTTTCTGGAAGCTTTTGTCATCTTTTGTGGCTTGGGGATCCAGAACACGCAGATGTA
  TGAAGCAGTGGAGAGAGCCATGGCCAAGCAAATGGTCACATTGGAGGTTCTGTCGTATCATGCTTCAGCA
  GCAGAGGAAGAAACAAGAGAGCTACAGTCGTTAGCGGCTGCTGTGGTGCCATCTGCCCAGACCCTTAAAA
  TTACTGACTTTAGCTTCAGTGACTTTGAGCTGTCTGATCTGGAAACAGCACTGTGTACAATTCGGATGTT
  TACTGACCTCAACCTTGTGCAGAACTTCCAGATGAAACATGAGGTTCTTTGCAGATGGATTTTAAGTGTT
  AAGAAGAATTATCGGAAGAATGTTGCCTATCATAATTGGAGACATGCCTTTAATACAGCTCAGTGCATGT
  TTGCTGCTCTAAAAGCAGGCAAAATTCAGAACAAGCTGACTGACCTGGAGATACTTGCATTGCTGATTGC
  TGCACTAAGCCACGATTTGGATCACCGTGGTGTGAATAACTCTTACATACAGCGAAGTGAACATCCACTT
  GCCCAGCTTTACTGCCATTCAATCATGGAACACCATCATTTTGACCAGTGCCTGATGATTCTTAATAGTC
  CAGGCAATCAGATTCTCAGTGGCCTCTCCATTGAAGAATATAAGACCACGTTGAAAATAATCAAGCAAGC
  TATTTTAGCTACAGACCTAGCACTGTACATTAAGAGGCGAGGAGAATTTTTTGAACTTATAAGAAAAAAT
  CAATTCAATTTGGAAGATCCTCATCAAAAGGAGTTGTTTTTGGCAATGCTGATGACAGCTTGTGATCTTT
  CTGCAATTACAAAACCCTGGCCTATTCAACAACGGATAGCAGAACTTGTAGCAACTGAATTTTTTGATCA
  AGGAGACAGAGAGAGAAAAGAACTCAACATAGAACCCACTGATCTAATGAACAGGGAGAAGAAAAACAAA
  ATCCCAAGTATGCAAGTTGGGTTCATAGATGCCATCTGCTTGCAACTGTATGAGGCCCTGACCCACGTGT
  CAGAGGACTGTTTCCCTTTGCTAGATGGCTGCAGAAAGAACAGGCAGAAATGGCAGGCCCTTGCAGAACA
  GCAGGAGAAGATGCTGATTAATGGGGAAAGCGGCCAGGCCAAGCGGAACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033430 unedited
  GGCCGCGAATTCGGCACCAGCCTCACCTCACCTCACCTGCGCGCGTTGTTGCCCCTACGG
  AGCCTCCTGGAGTCCAGGATCGGCGGAGTTCGAAAACGAACTTCCCACGTTTGCTATGTT
  GCCCTTTGGAGACAAAACAAGAGAAATGGTCAATGCATGGTTTGCTGAGAGAGTTCACAC
  CATCCCTGTGTGCAAGGAAGGTATCAGAGGCCACACCGAATCTTGCTCTTGTCCCTTGCA
  GCAGAGTCCTCGTGCAGATAACAGTGTCCCTGGAACACCAACCAGGAAAATCTCTGCCTC
  TGAATTTGACCGGCCTCTTAGACCCATTGTTGTCAAGGATTCTGAGGGAACTGTGAGCTT
  CCTCTCTGACTCAGAAAAGAAGGAACAGATGCCTCTAACCCCTCCAAGGTTTGATCATGA
  TGAAGGGGACCAGTGCTCAAGACTCTTGGAATTAGTGAAGGATATTTCTAGTCATTTGGA
  TGTCACAGCCTTATGTCACAAAATTTTCTTGCATATCCATGGACTGATATCTGCTGACCG
  CTATTCCCTGTTCCTTGTCTGTGAAGACAGCTCCAATGACAAGTTTCTTATCAGCCGCCT
  CTTTGATGTTGCTGAAGGTTCAACACTGGAAGAAGTTTCAAATAACTGTATCCGCTTAGA
  ATGGAACANAGGCATTGTGGGACATGTGGCAGCGCTTGGTGAGCCCTTGACATCAAGATG
  CATATGAGGATCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_033430 unedited
  TTGTCGCGTTCTATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGCCTCA
  CCTCACCTCACCTGCGCGCGTTGTTGCCCCTACGGAGCCTCCTGGAGTCCAGGATCGGCG
  GAGTTCGAAAACGAACTTCCCACGTTTGCTATGTTGCCCTTTGGAGACAAAACAAGAGAA
  ATGGTCAATGCATGGTTTGCTGAGAGAGTTCACACCATCCCTGTGTGCAAGGAAGGTATC
  AGAGGCCACACCGAATCTTGCTCTTGTCCCTTGCAGCAGAGTCCTCGTGCAGATAACAGT
  GTCCCTGGAACACCAACCAGGAAAATCTCTGCCTCTGAATTTGACCGGCCTCTTAGACCC
  ATTGTTGTCAAGGATTCTGAGGGAACTGTGAGCTTCCTCTCTGACTCAGAAAAGAAGGAA
  CAGATGCCTCTAACCCCTCCAAGGTTTGATCATGATGAAGGGGACCAGTGCTCAAGACTC
  TTGGAATTAGTGAAGGATATTTCTAGTCATTTGGATGTCACAGCCTTATGTCACAAAATT
  TTCTTGCATATCCATGGACTGATATCTGCTGACCGCTATTCCCTGTTCCTTGTCTGTGAA
  GACAGCTCCAATGACAAGTTTCTTATCAGCCGCCTCTTTGATGTTGCTGAAGGTTCAACA
  CTGGAAGAAGTTTCANATAACTGTATCCGCTTAGAATGGAACAAAGGCATTGTGGGACAT
  GTGGCAGCGCTTGGTGAGCCCTTTGACATCANAGATGCATATGANGATCCCTCGTTCAAT
  GCAGAAGTTGACCAAATTACANNGCTACAGACACAAAGCATTCTTTGTATGCCAATTAAG
  AATCATAAGGGAAGAGGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033430
• ORF Size: 2502 bp
• Insert Size: 5000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_033430.2, NP_236914.2
• RefSeq Size: 6836
• RefSeq ORF: 2502
• Locus ID: 8654
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (2, also known as PDE5A2) has a distinct and shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.