THEMIS (NM_001010923) Human Tagged ORF Clone

CAT#: RG219162

THEMIS (GFP-tagged) - Human thymocyte selection associated (THEMIS), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: THEMIS
• Synonyms: C6orf190; C6orf207; GASP; SPOT; TSEPA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219162 representing NM_001010923
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCATTATCACTGGAAGAATTCGTCCACTCCCTTGACCTCAGGACCCTACCCAGGGTTCTAGAAATCC
  AGGCAGGCATCTATCTTGAAGGCTCTATTTATGAAATGTTTGGAAATGAATGCTGTTTTTCAACAGGAGA
  AGTGATTAAAATTACTGGTCTCAAAGTTAAGAAGATCATAGCTGAAATTTGTGAGCAGATTGAAGGTTGT
  GAGTCTCTACAGCCATTTGAACTGCCTATGAATTTTCCAGGTCTTTTTAAGATTGTGGCTGATAAAACTC
  CATACCTTACTATGGAAGAAATCACAAGGACCATTCATATTGGACCAAGTAGACTAGGGCATCCTTGCTT
  CTATCATCAGAAGGATATAAAACTAGAGAACCTCATCATAAAGCAGGGTGAGCAAATCATGCTCAACTCA
  GTTGAAGAGATTGATGGAGAAATAATGGTGAGCTGTGCAGTAGCAAGGAATCATCAAACTCACTCATTTA
  ATTTGCCTTTGTCACAAGAAGGAGAATTCTACGAGTGTGAAGATGAACGTATTTACACTCTAAAGGAGAT
  TGTTGAATGGAAGATTCCTAAGAACAGAACAAGAACTGTAAACCTTACAGATTTTTCAAATAAGTGGGAC
  TCAACGAATCCATTTCCTAAAGACTTTTATGGTACCCTGATTCTCAAGCCTGTTTATGAAATTCAAGGTG
  TGATGAAATTTCGAAAAGATATAATCCGCATCCTCCCCAGTCTAGATGTCGAAGTCAAAGACATCACTGA
  TTCTTACGATGCTAACTGGTTTCTTCAGCTGTTATCAACAGAAGATCTTTTTGAAATGACTAGTAAAGAG
  TTCCCCATAGTGACTGAAGTCATAGAAGCACCTGAAGGAAACCACCTGCCCCAAAGCATTTTACAGCCTG
  GGAAAACCATTGTGATCCACAAAAAGTACCAGGCATCAAGAATCTTAGCTTCAGAAATTAGAAGCAATTT
  TCCTAAAAGACACTTCTTGATCCCCACTAGCTATAAAGGCAAGTTCAAGCGGCGACCGAGGGAGTTCCCA
  ACGGCCTATGACCTAGAGATCGCTAAGAGTGAAAAGGAGCCTCTTCACGTGGTGGCCACCAAAGCGTTTC
  ATTCCCCTCATGACAAGCTGTCATCCGTATCTGTTGGGGACCAGTTTCTGGTGCATCAGTCAGAGACGAC
  TGAAGTCCTCTGTGAGGGAATAAAAAAAGTGGTGAATGTTCTGGCCTGTGAAAAAATCCTCAAAAAGTCC
  TATGAGGCTGCGCTGCTCCCTTTGTACATGGAAGGAGGTTTTGTAGAGGTGATTCATGATAAGAAACAGT
  ACCCGATTTCTGAGCTCTGTAAACAGTTCCGTTTGCCCTTCAATGTGAAGGTGTCTGTCAGGGATCTTTC
  CATTGAAGAGGACGTGTTGGCTGCCACACCAGGACTGCAGTTGGAGGAGGACATTACAGACTCTTACCTA
  CTCATAAGTGACTTTGCCAACCCCACGGAGTGCTGGGAAATTCCTGTGGGCCGCTTGAATATGACTGTTC
  AGTTAGTTAGTAATTTCTCTAGGGATGCAGAACCATTTCTAGTCAGGACTCTGGTAGAAGAGATCACTGA
  AGAGCAATATTACATGATGCGGAGATATGAAAGCTCAGCCTCACATCCCCCACCTCGCCCTCCGAAACAC
  CCCTCAGTAGAGGAAACAAAGTTAACCCTGCTAACCTTAGCAGAAGAAAGGACGGTAGACCTGCCCAAGT
  CTCCCAAGCGTCATCACGTAGACATAACCAAGAAACTTCACCCAAATCAAGCTGGCCTGGATTCAAAAGT
  ACTGATTGGTAGTCAGAATGATTTGGTGGATGAAGAGAAAGAAAGGAGCAACCGTGGGGCCACAGCAATA
  GCAGAAACATTCAAAAATGAAAAACATCAAAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219162 representing NM_001010923
  Red=Cloning site Green=Tags(s)
  
  MALSLEEFVHSLDLRTLPRVLEIQAGIYLEGSIYEMFGNECCFSTGEVIKITGLKVKKIIAEICEQIEGC
  ESLQPFELPMNFPGLFKIVADKTPYLTMEEITRTIHIGPSRLGHPCFYHQKDIKLENLIIKQGEQIMLNS
  VEEIDGEIMVSCAVARNHQTHSFNLPLSQEGEFYECEDERIYTLKEIVEWKIPKNRTRTVNLTDFSNKWD
  STNPFPKDFYGTLILKPVYEIQGVMKFRKDIIRILPSLDVEVKDITDSYDANWFLQLLSTEDLFEMTSKE
  FPIVTEVIEAPEGNHLPQSILQPGKTIVIHKKYQASRILASEIRSNFPKRHFLIPTSYKGKFKRRPREFP
  TAYDLEIAKSEKEPLHVVATKAFHSPHDKLSSVSVGDQFLVHQSETTEVLCEGIKKVVNVLACEKILKKS
  YEAALLPLYMEGGFVEVIHDKKQYPISELCKQFRLPFNVKVSVRDLSIEEDVLAATPGLQLEEDITDSYL
  LISDFANPTECWEIPVGRLNMTVQLVSNFSRDAEPFLVRTLVEEITEEQYYMMRRYESSASHPPPRPPKH
  PSVEETKLTLLTLAEERTVDLPKSPKRHHVDITKKLHPNQAGLDSKVLIGSQNDLVDEEKERSNRGATAI
  AETFKNEKHQK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001010923
• ORF Size: 1923 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001010923.1, NP_001010923.1
• RefSeq Size: 4039
• RefSeq ORF: 1926
• Locus ID: 387357
• Gene Summary: This gene encodes a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]