GPR39 (NM_001508) Human Tagged ORF Clone

CAT#: RG219212

GPR39 (GFP-tagged) - Human G protein-coupled receptor 39 (GPR39)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: GPR39
• Synonyms: MGC149541
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219212 representing NM_001508
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTCACCCAGCCTCCCGGGCAGTGACTGCTCCCAAATCATTGATCACAGTCATGTCCCCGAGTTTG
  AGGTGGCCACCTGGATCAAAATCACCCTTATTCTGGTGTACCTGATCATCTTCGTGATGGGCCTTCTGGG
  GAACAGCGCCACCATTCGGGTCACCCAGGTGCTGCAGAAGAAAGGATACTTGCAGAAGGAGGTGACAGAC
  CACATGGTGAGTTTGGCTTGCTCGGACATCTTGGTGTTCCTCATCGGCATGCCCATGGAGTTCTACAGCA
  TCATCTGGAATCCCCTGACCACGTCCAGCTACACCCTGTCCTGCAAGCTGCACACTTTCCTCTTCGAGGC
  CTGCAGCTACGCTACGCTGCTGCACGTGCTGACACTCAGCTTTGAGCGCTACATCGCCATCTGTCACCCC
  TTCAGGTACAAGGCTGTGTCGGGACCTTGCCAGGTGAAGCTGCTGATTGGCTTCGTCTGGGTCACCTCCG
  CCCTGGTGGCACTGCCCTTGCTGTTTGCCATGGGTACTGAGTACCCCCTGGTGAACGTGCCCAGCCACCG
  GGGTCTCACTTGCAACCGCTCCAGCACCCGCCACCACGAGCAGCCCGAGACCTCCAATATGTCCATCTGT
  ACCAACCTCTCCAGCCGCTGGACCGTGTTCCAGTCCAGCATCTTCGGCGCCTTCGTGGTCTACCTCGTGG
  TCCTGCTCTCCGTAGCCTTCATGTGCTGGAACATGATGCAGGTGCTCATGAAAAGCCAGAAGGGCTCGCT
  GGCCGGGGGCACGCGGCCTCCGCAGCTGAGGAAGTCCGAGAGCGAAGAGAGCAGGACCGCCAGGAGGCAG
  ACCATCATCTTCCTGAGGCTGATTGTTGTGACATTGGCCGTATGCTGGATGCCCAACCAGATTCGGAGGA
  TCATGGCTGCGGCCAAACCCAAGCACGACTGGACGAGGTCCTACTTCCGGGCGTACATGATCCTCCTCCC
  CTTCTCGGAGACGTTTTTCTACCTCAGCTCGGTCATCAACCCGCTCCTGTACACGGTGTCCTCGCAGCAG
  TTTCGGCGGGTGTTCGTGCAGGTGCTGTGCTGCCGCCTGTCGCTGCAGCACGCCAACCACGAGAAGCGCC
  TGCGCGTACATGCGCACTCCACCACCGACAGCGCCCGCTTTGTGCAGCGCCCGTTGCTCTTCGCGTCCCG
  GCGCCAGTCCTCTGCAAGGAGAACTGAGAAGATTTTCTTAAGCACTTTTCAGAGCGAGGCCGAGCCCCAG
  TCTAAGTCCCAGTCATTGAGTCTCGAGTCACTAGAGCCCAACTCAGGCGCGAAACCAGCCAATTCTGCTG
  CAGAGAATGGTTTTCAGGAGCATGAAGTTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219212 representing NM_001508
  Red=Cloning site Green=Tags(s)
  
  MASPSLPGSDCSQIIDHSHVPEFEVATWIKITLILVYLIIFVMGLLGNSATIRVTQVLQKKGYLQKEVTD
  HMVSLACSDILVFLIGMPMEFYSIIWNPLTTSSYTLSCKLHTFLFEACSYATLLHVLTLSFERYIAICHP
  FRYKAVSGPCQVKLLIGFVWVTSALVALPLLFAMGTEYPLVNVPSHRGLTCNRSSTRHHEQPETSNMSIC
  TNLSSRWTVFQSSIFGAFVVYLVVLLSVAFMCWNMMQVLMKSQKGSLAGGTRPPQLRKSESEESRTARRQ
  TIIFLRLIVVTLAVCWMPNQIRRIMAAAKPKHDWTRSYFRAYMILLPFSETFFYLSSVINPLLYTVSSQQ
  FRRVFVQVLCCRLSLQHANHEKRLRVHAHSTTDSARFVQRPLLFASRRQSSARRTEKIFLSTFQSEAEPQ
  SKSQSLSLESLEPNSGAKPANSAAENGFQEHEV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001508
• ORF Size: 1361 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001508.1, NP_001499.1
• RefSeq Size: 1362 bp
• RefSeq ORF: 1362 bp
• Locus ID: 2863
• Cytogenetics: 2q21.2
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Gene Summary: 'This gene is a member of the ghrelin receptor family and encodes a rhodopsin-type G-protein-coupled receptor (GPCR). The encoded protein is involved in zinc-dependent signaling in epithelial tissue in intestines, prostate and salivary glands. The protein may also be involved in the pathophysiology of depression. [provided by RefSeq, Jun 2016]'