BCL9 (NM_004326) Human Tagged ORF Clone

CAT#: RG219304

BCL9 (GFP-tagged) - Human B-cell CLL/lymphoma 9 (BCL9)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: BCL9
• Synonyms: LGS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219304 representing NM_004326
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATTCCAGTAACCCTAAAGTGAGGAGCTCTCCATCAGGAAACACACAGAGTAGCCCTAAGTCAAAGC
  AGGAGGTGATGGTCCGTCCCCCTACAGTGATGTCCCCATCTGGAAACCCCCAGCTGGATTCCAAATTCTC
  CAATCAGGGTAAACAGGGGGGCTCAGCCAGCCAATCCCAGCCATCCCCCTGTGACTCCAAGAGTGGGGGC
  CATACCCCTAAAGCACTCCCTGGCCCAGGTGGGAGCATGGGGCTGAAGAATGGGGCTGGAAATGGTGCCA
  AGGGCAAGGGGAAAAGGGAGCGAAGTATTTCCGCCGACTCCTTTGATCAGAGAGATCCTGGGACTCCAAA
  CGATGACTCTGACATTAAAGAATGTAATTCTGCTGACCACATAAAGTCCCAGGATTCCCAGCACACACCA
  CACTCGATGACCCCATCAAATGCTACAGCCCCCAGGTCTTCTACCCCCTCCCATGGCCAAACTACTGCCA
  CAGAGCCCACACCTGCTCAGAAGACTCCAGCCAAAGTGGTGTACGTGTTTTCTACTGAGATGGCCAATAA
  AGCTGCAGAAGCTGTTTTGAAGGGCCAGGTTGAAACTATCGTCTCTTTCCACATCCAGAACATTTCTAAC
  AACAAGACAGAGAGAAGCACAGCGCCTCTGAACACACAGATATCTGCCCTTCGGAATGATCCGAAACCTC
  TCCCACAACAGCCCCCAGCTCCGGCCAACCAGGACCAGAATTCTTCCCAGAATACCAGACTGCAGCCAAC
  TCCACCCATTCCGGCACCAGCACCCAAGCCTGCCGCACCCCCACGTCCCCTGGACCGGGAGAGTCCTGGG
  GTAGAAAACAAACTGATTCCTTCTGTAGGAAGTCCTGCCAGCTCCACTCCACTGCCCCCAGATGGTACTG
  GGCCCAACTCAACTCCCAACAATAGGGCAGTGACCCCTGTCTCCCAGGGGAGCAATAGCTCTTCAGCAGA
  TCCCAAAGCCCCTCCGCCTCCACCAGTGTCCAGTGGCGAGCCCCCCACACTGGGAGAGAATCCCGATGGC
  CTATCTCAGGAGCAGCTGGAGCACCGGGAGCGCTCCTTACAAACTCTCAGAGATATCCAGCGCATGCTTT
  TTCCTGATGAGAAAGAATTCACAGGAGCACAAAGTGGGGGACCGCAGCAGAATCCTGGGGTATTAGATGG
  GCCTCAGAAAAAACCAGAAGGGCCAATACAGGCCATGATGGCCCAATCCCAAAGCCTAGGTAAGGGACCT
  GGGCCCCGGACAGACGTGGGAGCTCCATTTGGCCCTCAAGGACATAGAGATGTACCCTTTTCTCCAGATG
  AAATGGTTCCACCTTCTATGAACTCCCAGTCTGGGACCATAGGACCCGACCACCTTGACCATATGACTCC
  CGAGCAGATAGCGTGGCTGAAACTGCAGCAGGAGTTTTATGAAGAGAAGAGGAGGAAGCAGGAACAAGTG
  GTTGTCCAGCAGTGTTCCCTCCAGGACATGATGGTCCATCAGCACGGGCCTCGGGGAGTGGTCCGAGGAC
  CCCCCCCTCCATACCAGATGACCCCTAGTGAAGGCTGGGCACCTGGGGGTACAGAGCCATTTTCTGATGG
  TATCAACATGCCACATTCTCTGCCCCCGAGGGGCATGGCTCCCCACCCCAACATGCCAGGGAGCCAGATG
  CGCCTCCCTGGATTTGCAGGCATGATAAACTCTGAAATGGAAGGGCCGAATGTCCCCAACCCTGCATCTA
  GACCAGGTCTTTCTGGAGTCAGTTGGCCAGATGATGTGCCAAAAATCCCAGATGGTCGAAATTTTCCTCC
  TGGCCAGGGCATTTTCAGCGGTCCTGGCCGAGGGGAACGCTTCCCAAACCCCCAAGGATTGTCTGAAGAG
  ATGTTTCAGCAGCAGCTGGCAGAGAAACAGCTGGGTCTCCCCCCAGGGATGGCCATGGAAGGCATCAGGC
  CCAGCATGGAGATGAACAGGATGATTCCAGGCTCCCAGCGCCACATGGAGCCTGGGAATAACCCCATTTT
  CCCTCGAATACCAGTTGAGGGCCCTCTGAGTCCTTCTAGGGGTGACTTTCCAAAAGGAATTCCCCCACAG
  ATGGGCCCTGGTCGGGAACTTGAGTTTGGGATGGTTCCTAGTGGGATGAAGGGAGATGTCAATCTAAATG
  TCAACATGGGATCCAACTCTCAGATGATACCTCAGAAGATGAGAGAGGCTGGGGCGGGCCCTGAGGAGAT
  GCTGAAATTACGCCCAGGTGGCTCAGACATGCTGCCTGCTCAGCAGAAGATGGTGCCACTGCCATTTGGT
  GAGCACCCCCAGCAGGAGTATGGCATGGGCCCCAGACCATTCCTTCCCATGTCTCAGGGTCCAGGCAGCA
  ACAGTGGCTTGCGGAATCTCAGAGAACCAATTGGGCCCGACCAGAGGACTAACAGCCGGCTCAGTCATAT
  GCCACCACTACCTCTCAACCCTTCCAGTAACCCCACCAGCCTCAACACAGCTCCTCCAGTTCAGCGCGGC
  CTGGGGCGGAAGCCCTTGGATATATCTGTGGCAGGCAGCCAGGTGCATTCCCCAGGCATTAACCCTCTGA
  AGTCTCCCACGATGCACCAAGTCCAGTCACCAATGCTGGGCTCGCCCTCGGGGAACCTCAAGTCCCCCCA
  GACTCCATCGCAGCTGGCAGGCATGCTGGCGGGCCCAGCTGCTGCTGCTTCCATTAAGTCCCCCCCTGTT
  TTGGGGTCTGCTGCTGCTTCACCTGTCCACCTCAAGTCTCCATCACTTCCTGCCCCGTCACCTGGATGGA
  CCTCTTCTCCAAAACCTCCCCTTCAGAGTCCTGGGATCCCTCCAAACCATAAAGCACCCCTCACCATGGC
  CTCCCCAGCCATGCTGGGAAATGTAGAGTCAGGTGGCCCCCCACCTCCTACAGCCAGCCAGCCTGCCTCT
  GTGAATATCCCTGGAAGTCTTCCCTCTAGTACACCTTATACCATGCCTCCAGAGCCAACCCTTTCCCAGA
  ACCCACTCTCTATTATGATGTCTCGAATGTCCAAGTTTGCAATGCCCAGTTCCACCCCGTTATACCATGA
  TGCTATCAAGACTGTGGCCAGCTCAGATGACGACTCCCCTCCAGCTCGTTCTCCCAACTTGCCATCAATG
  AATAATATGCCAGGAATGGGCATTAATACACAGAATCCTCGAATTTCAGGTCCAAACCCCGTGGTTCCGA
  TGCCAACCCTCAGCCCAATGGGAATGACCCAGCCACTTTCTCACTCCAATCAGATGCCCTCTCCAAATGC
  CGTGGGACCCAACATACCTCCTCATGGGGTCCCAATGGGGCCTGGCTTGATGTCACACAATCCTATCATG
  GGGCATGGGTCCCAGGAGCCACCGATGGTACCTCAAGGACGGATGGGCTTCCCCCAGGGCTTCCCTCCAG
  TACAGTCTCCCCCACAGCAGGTTCCATTCCCTCACAATGGCCCCAGTGGGGGGCAGGGCAGCTTCCCAGG
  AGGGATGGGTTTCCCAGGAGAAGGCCCCCTTGGCCGCCCCAGCAACCTGCCCCAAAGTTCAGCAGATGCA
  GCACTTTGCAAGCCTGGAGGCCCCGGGGGTCCTGACTCCTTCACTGTCCTGGGGAACAGCATGCCTTCGG
  TGTTTACAGACCCAGATCTGCAGGAGGTCATCCGACCTGGAGCCACCGGAATACCTGAGTTTGATCTATC
  CCGCATTATTCCATCTGAGAAGCCCAGCCAGACGCTGCAATATTTCCCTCGAGGGGAAGTTCCAGGCCGT
  AAACAGCCCCAGGGTCCTGGACCTGGGTTTTCACACATGCAGGGGATGATGGGCGAACAAGCCCCCAGAA
  TGGGACTAGCATTACCTGGCATGGGAGGTCCAGGGCCAGTGGGAACTCCGGACATCCCTCTTGGTACAGC
  TCCATCCATGCCAGGCCACAACCCCATGAGACCACCAGCCTTTCTCCAACAAGGCATGATGGGACCTCAC
  CATCGGATGATGTCACCAGCACAATCTACAATGCCCGGCCAGCCCACCCTGATGAGCAATCCAGCTGCTG
  CCGTGGGCATGATTCCTGGCAAGGATCGGGGGCCTGCCGGGCTCTACACCCACCCTGGGCCTGTGGGCTC
  TCCAGGCATGATGATGTCCATGCAGGGCATGATGGGACCCCAACAGAACATCATGATCCCCCCACAGATG
  AGGCCCCGGGGCATGGCTGCTGACGTGGGCATGGGTGGATTTAGCCAAGGACCTGGCAACCCAGGAAACA
  TGATGTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219304 representing NM_004326
  Red=Cloning site Green=Tags(s)
  
  MHSSNPKVRSSPSGNTQSSPKSKQEVMVRPPTVMSPSGNPQLDSKFSNQGKQGGSASQSQPSPCDSKSGG
  HTPKALPGPGGSMGLKNGAGNGAKGKGKRERSISADSFDQRDPGTPNDDSDIKECNSADHIKSQDSQHTP
  HSMTPSNATAPRSSTPSHGQTTATEPTPAQKTPAKVVYVFSTEMANKAAEAVLKGQVETIVSFHIQNISN
  NKTERSTAPLNTQISALRNDPKPLPQQPPAPANQDQNSSQNTRLQPTPPIPAPAPKPAAPPRPLDRESPG
  VENKLIPSVGSPASSTPLPPDGTGPNSTPNNRAVTPVSQGSNSSSADPKAPPPPPVSSGEPPTLGENPDG
  LSQEQLEHRERSLQTLRDIQRMLFPDEKEFTGAQSGGPQQNPGVLDGPQKKPEGPIQAMMAQSQSLGKGP
  GPRTDVGAPFGPQGHRDVPFSPDEMVPPSMNSQSGTIGPDHLDHMTPEQIAWLKLQQEFYEEKRRKQEQV
  VVQQCSLQDMMVHQHGPRGVVRGPPPPYQMTPSEGWAPGGTEPFSDGINMPHSLPPRGMAPHPNMPGSQM
  RLPGFAGMINSEMEGPNVPNPASRPGLSGVSWPDDVPKIPDGRNFPPGQGIFSGPGRGERFPNPQGLSEE
  MFQQQLAEKQLGLPPGMAMEGIRPSMEMNRMIPGSQRHMEPGNNPIFPRIPVEGPLSPSRGDFPKGIPPQ
  MGPGRELEFGMVPSGMKGDVNLNVNMGSNSQMIPQKMREAGAGPEEMLKLRPGGSDMLPAQQKMVPLPFG
  EHPQQEYGMGPRPFLPMSQGPGSNSGLRNLREPIGPDQRTNSRLSHMPPLPLNPSSNPTSLNTAPPVQRG
  LGRKPLDISVAGSQVHSPGINPLKSPTMHQVQSPMLGSPSGNLKSPQTPSQLAGMLAGPAAAASIKSPPV
  LGSAAASPVHLKSPSLPAPSPGWTSSPKPPLQSPGIPPNHKAPLTMASPAMLGNVESGGPPPPTASQPAS
  VNIPGSLPSSTPYTMPPEPTLSQNPLSIMMSRMSKFAMPSSTPLYHDAIKTVASSDDDSPPARSPNLPSM
  NNMPGMGINTQNPRISGPNPVVPMPTLSPMGMTQPLSHSNQMPSPNAVGPNIPPHGVPMGPGLMSHNPIM
  GHGSQEPPMVPQGRMGFPQGFPPVQSPPQQVPFPHNGPSGGQGSFPGGMGFPGEGPLGRPSNLPQSSADA
  ALCKPGGPGGPDSFTVLGNSMPSVFTDPDLQEVIRPGATGIPEFDLSRIIPSEKPSQTLQYFPRGEVPGR
  KQPQGPGPGFSHMQGMMGEQAPRMGLALPGMGGPGPVGTPDIPLGTAPSMPGHNPMRPPAFLQQGMMGPH
  HRMMSPAQSTMPGQPTLMSNPAAAVGMIPGKDRGPAGLYTHPGPVGSPGMMMSMQGMMGPQQNIMIPPQM
  RPRGMAADVGMGGFSQGPGNPGNMMF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004326
• ORF Size: 4278 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004326.2, NP_004317.2
• RefSeq Size: 6278 bp
• RefSeq ORF: 4281 bp
• Locus ID: 607
• Cytogenetics: 1q21.2
• Protein Families: Druggable Genome
• Gene Summary: 'BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies. [provided by RefSeq, Jul 2008]'