DUSP18 (NM_152511) Human Tagged ORF Clone

CAT#: RG219496

DUSP18 (GFP-tagged) - Human dual specificity phosphatase 18 (DUSP18)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DUSP18
• Synonyms: DSP18; DUSP20; LMWDSP20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219496 representing NM_152511
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGCACCCTCGTGTGCCTTCCCAGTTCAGTTCCGGCAGCCCTCAGTCAGCGGCCTCTCGCAGATAA
  CCAAAAGCCTGTATATCAGCAATGGTGTGGCCGCCAACAACAAGCTCATGCTGTCTAGCAACCAGATCAC
  CATGGTCATCAATGTCTCAGTGGAGGTAGTGAACACCTTGTATGAGGATATCCAGTACATGCAGGTACCT
  GTGGCTGACTCCCCTAACTCACGTCTCTGTGACTTCTTTGACCCTATTGCTGACCATATCCACAGCGTGG
  AGATGAAGCAGGGCCGTACTTTGCTGCACTGTGCTGCTGGTGTGAGCCGCTCAGCTGCCCTGTGCCTCGC
  CTACCTCATGAAGTACCACGCCATGTCCCTGCTGGACGCCCACACGTGGACCAAGTCATGCCGGCCCATC
  ATCCGACCCAACAGCGGCTTTTGGGAGCAGCTCATCCACTATGAGTTCCAATTGTTTGGCAAGAACACTG
  TGCACATGGTCAGTTCCCCAGTGGGAATGATCCCTGACATCTATGAGAAGGAAGTCCGTTTGATGATTCC
  ACTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219496 representing NM_152511
  Red=Cloning site Green=Tags(s)
  
  MTAPSCAFPVQFRQPSVSGLSQITKSLYISNGVAANNKLMLSSNQITMVINVSVEVVNTLYEDIQYMQVP
  VADSPNSRLCDFFDPIADHIHSVEMKQGRTLLHCAAGVSRSAALCLAYLMKYHAMSLLDAHTWTKSCRPI
  IRPNSGFWEQLIHYEFQLFGKNTVHMVSSPVGMIPDIYEKEVRLMIPL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152511
• ORF Size: 564 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_152511.3, NP_689724.3
• RefSeq Size: 2453
• RefSeq ORF: 567
• Locus ID: 150290
• Domains: DSPc
• Protein Families: Druggable Genome, Phosphatase
• Gene Summary: Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. They have been implicated as major modulators of critical signaling pathways. DUSP18 contains the consensus DUSP C-terminal catalytic domain but lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase phosphatase) class of DUSPs (see MIM 600714) (summary by Patterson et al., 2009 [PubMed 19228121]). [supplied by OMIM, Dec 2009]