TEAD4 (NM_003213) Human Tagged ORF Clone

CAT#: RG219686

TEAD4 (GFP-tagged) - Human TEA domain family member 4 (TEAD4), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TEAD4
• Synonyms: EFTR-2; hRTEF-1B; RTEF1; TCF13L1; TEF-3; TEF3; TEFR-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219686 representing NM_003213
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  TTGGAGGGCACGGCCGGCACCATTACCTCCAACGAGTGGAGCTCTCCCACCTCCCCTGAGGGGAGCACCG
  CCTCTGGGGGCAGTCAGGCACTGGACAAGCCCATCGACAATGACGCAGAGGGCGTGTGGAGCCCGGATAT
  TGAGCAGAGTTTCCAGGAGGCCCTCGCCATCTACCCGCCCTGTGGCAGGCGCAAAATCATCCTGTCGGAC
  GAGGGCAAGATGTATGGTCGGAACGAGCTGATTGCCCGCTACATCAAGCTCCGGACAGGGAAGACCCGCA
  CCAGGAAGCAGGTCTCCAGCCACATCCAGGTGCTGGCTCGTCGCAAAGCTCGCGAGATCCAGGCCAAGCT
  AAAGGACCAGGCAGCTAAGGACAAGGCCCTGCAGAGCATGGCTGCCATGTCGTCTGCACAGATCATCTCC
  GCCACGGCCTTCCACAGTAGCATGGCCCTCGCCCGGGGCCCCGGCCGCCCAGCAGTCTCAGGGTTTTGGC
  AAGGAGCTTTGCCAGGCCAAGCCGGAACGTCCCATGATGTGAAGCCTTTCTCTCAGCAAACCTATGCTGT
  CCAGCCTCCGCTGCCTCTGCCAGGGTTTGAGTCTCCTGCAGGGCCCGCCCCATCGCCCTCTGCGCCCCCG
  GCACCCCCATGGCAGGGCCGCAGCGTGGCCAGCTCCAAGCTCTGGATGTTGGAGTTCTCTGCCTTCCTGG
  AGCAGCAGCAGGACCCGGACACGTACAACAAGCACCTGTTCGTGCACATTGGCCAGTCCAGCCCAAGCTA
  CAGCGACCCCTACCTCGAAGCCGTGGACATCCGCCAAATCTATGACAAATTCCCGGAGAAAAAGGGTGGA
  CTCAAGGATCTCTTCGAACGGGGACCCTCCAATGCCTTTTTTCTTGTGAAGTTCTGGGCAGACCTCAACA
  CCAACATCGAGGATGAAGGCAGCTCCTTCTATGGGGTCTCCAGCCAGTATGAGAGCCCCGAGAACATGAT
  CATCACCTGCTCCACGAAGGTCTGCTCTTTCGGCAAGCAGGTGGTGGAGAAAGTTGAGACAGAGTATGCT
  CGCTATGAGAATGGACACTACTCTTACCGCATCCACCGGTCCCCGCTCTGTGAGTACATGATCAACTTCA
  TCCACAAGCTCAAGCACCTCCCTGAGAAGTACATGATGAACAGCGTGCTGGAGAACTTCACCATCCTGCA
  GGTGGTCACCAACAGAGACACACAGGAGACCTTGCTGTGCATTGCCTATGTCTTTGAGGTGTCAGCCAGT
  GAGCACGGGGCTCAGCACCACATCTACAGGCTGGTGAAAGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219686 representing NM_003213
  Red=Cloning site Green=Tags(s)
  
  LEGTAGTITSNEWSSPTSPEGSTASGGSQALDKPIDNDAEGVWSPDIEQSFQEALAIYPPCGRRKIILSD
  EGKMYGRNELIARYIKLRTGKTRTRKQVSSHIQVLARRKAREIQAKLKDQAAKDKALQSMAAMSSAQIIS
  ATAFHSSMALARGPGRPAVSGFWQGALPGQAGTSHDVKPFSQQTYAVQPPLPLPGFESPAGPAPSPSAPP
  APPWQGRSVASSKLWMLEFSAFLEQQQDPDTYNKHLFVHIGQSSPSYSDPYLEAVDIRQIYDKFPEKKGG
  LKDLFERGPSNAFFLVKFWADLNTNIEDEGSSFYGVSSQYESPENMIITCSTKVCSFGKQVVEKVETEYA
  RYENGHYSYRIHRSPLCEYMINFIHKLKHLPEKYMMNSVLENFTILQVVTNRDTQETLLCIAYVFEVSAS
  EHGAQHHIYRLVKE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003213
• ORF Size: 1302 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003213.2, NP_003204.2
• RefSeq Size: 1780 bp
• RefSeq ORF: 1305 bp
• Locus ID: 7004
• Cytogenetics: 12p13.33
• Domains: TEA
• Protein Families: Transcription Factors
• Gene Summary: 'This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene. [provided by RefSeq, Jul 2008]'