VPS37C (NM_017966) Human Tagged ORF Clone

CAT#: RG219699

VPS37C (GFP-tagged) - Human vacuolar protein sorting 37 homolog C (S. cerevisiae) (VPS37C)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: VPS37C
• Synonyms: FLJ20847
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219699 representing NM_017966
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACGCTGAAGGATAAGACCCTGCAGGAGCTGGAGGAGTTGCAGAATGACTCGGAGGCGATTGACC
  AGCTGGCCCTGGAGTCCCCTGAGGTCCAGGACCTACAGCTGGAACGGGAGATGGCACTGGCCACCAACCG
  GAGCCTGGCAGAGCGGAACTTGGAGTTCCAGGGTCCCCTGGAGATCAGCCGCTCAAACCTCTCGGATAGA
  TACCAGGAGCTCCGGAAGCTCGTGGAGCGGTGCCAGGAGCAGAAGGCAAAGCTGGAGAAATTTTCTTCAG
  CACTGCAGCCAGGGACCTTGTTAGACCTTCTGCAGGTGGAAGGCATGAAGATCGAAGAAGAGTCCGAGGC
  CATGGCTGAGAAGTTCCTGGAGGGCGAGGTGCCCCTGGAAACGTTCCTGGAGAATTTTTCCTCCATGAGG
  ATGCTGTCCCACCTGCGCCGGGTTCGCGTGGAAAAGCTCCAGGAAGTGGTGAGGAAGCCCAGGGCTTCCC
  AGGAGCTGGCCGGCGATGCCCCTCCACCCCGTCCACCACCCCCGGTGCGCCCAGTCCCCCAGGGAACACC
  CCCTGTGGTTGAAGAGCAGCCGCAGCCACCATCAGCCATGCCTCCCTACCCTTTGCCCTACAGCCCATCC
  CCCAGCCTGCCTGTGGGCCCCACTGCCCATGGAGCCCTGCCACCGGCCCCTTTCCCAGTAGTGTCCCAGC
  CCTCCTTCTACAGCGGGCCTCTGGGCCCCACTTACCCGGCAGCCCAGCTTGGACCCAGGGGTGCTGCGGG
  TTACTCCTGGTCCCCACAGAGGAGCATGCCACCCCGGCCGGGCTATCCTGGGACCCCAATGGGTGCCTCT
  GGGCCTGGGTACCCCTTGCGGGGAGGCAGGGCCCCCAGTCCTGGTTATCCTCAACAGTCCCCATACCCCG
  CAACAGGAGGAAAACCTCCCTACCCAATACAGCCTCAGCTCCCCAGCTTTCCAGGCCAGCCCCAGCCCTC
  AGTGCCCCTGCAGCCCCCTTATCCCCCCGGGCCCGCCCCTCCCTATGGGTTCCCACCACCGCCGGGGCCT
  GCCTGGCCTGGGTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219699 representing NM_017966
  Red=Cloning site Green=Tags(s)
  
  METLKDKTLQELEELQNDSEAIDQLALESPEVQDLQLEREMALATNRSLAERNLEFQGPLEISRSNLSDR
  YQELRKLVERCQEQKAKLEKFSSALQPGTLLDLLQVEGMKIEEESEAMAEKFLEGEVPLETFLENFSSMR
  MLSHLRRVRVEKLQEVVRKPRASQELAGDAPPPRPPPPVRPVPQGTPPVVEEQPQPPSAMPPYPLPYSPS
  PSLPVGPTAHGALPPAPFPVVSQPSFYSGPLGPTYPAAQLGPRGAAGYSWSPQRSMPPRPGYPGTPMGAS
  GPGYPLRGGRAPSPGYPQQSPYPATGGKPPYPIQPQLPSFPGQPQPSVPLQPPYPPGPAPPYGFPPPPGP
  AWPGY
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017966
• ORF Size: 1065 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017966.4, NP_060436.4
• RefSeq Size: 2711
• RefSeq ORF: 1068
• Locus ID: 55048
• Protein Pathways: Endocytosis
• Gene Summary: VPS37C is a subunit of ESCRT-I (endosomal sorting complex required for transport I), a complex in the class E vacuolar protein sorting (VPS) pathway required for sorting ubiquitinated transmembrane proteins into internal vesicles of multivesicular bodies (Eastman et al., 2005 [PubMed 15509564]). [supplied by OMIM, Mar 2008]