NRAMP1 (SLC11A1) (NM_000578) Human Tagged ORF Clone

CAT#: RG219733

SLC11A1 (GFP-tagged) - Human solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1 (SLC11A1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC11A1
• Synonyms: LSH; NRAMP; NRAMP1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219733 representing NM_000578
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAGGTGACAAGGGTCCCCAAAGGCTAAGCGGGTCCAGCTATGGTTCCATCTCCAGCCCGACCAGCC
  CGACCAGCCCAGGGCCACGGCAAGCACCTCCCAGAGAGACCTACCTGAGTGAGAAGATCCCCATCCCAGA
  CACAAAACCGGGCACCTTCAGCCTGCGGAAGCTATGGGCCTTCACGGGGCCTGGCTTCCTCATGAGCATT
  GCTTTCCTGGACCCAGGAAACATCGAGTCAGATCTTCAGGCTGGCGCCGTGGCGGGATTCAAACTTCTCT
  GGGTGCTGCTCTGGGCCACCGTGTTGGGCTTGCTCTGCCAGCGACTGGCTGCACGTCTGGGCGTGGTGAC
  AGGCAAGGACTTGGGCGAGGTCTGCCATCTCTACTACCCTAAGGTGCCCCGCACCGTCCTCTGGCTGACC
  ATCGAGCTAGCCATTGTGGGCTCCGACATGCAGGAAGTCATCGGCACGGCCATTGCATTCAATCTGCTCT
  CAGCTGGACGAATCCCACTCTGGGGTGGCGTCCTCATCACCATCGTGGACACCTTCTTCTTCCTCTTCCT
  CGATAACTACGGGCTGCGGAAGCTGGAAGCTTTTTTTGGACTCCTTATAACCATTATGGCCTTGACCTTT
  GGCTATGAGTATGTGGTGGCGCGTCCTGAGCAGGGAGCGCTTCTTCGGGGCCTGTTCCTGCCCTCGTGCC
  CGGGCTGCGGCCACCCCGAGCTGCTGCAGGCGGTGGGCATTGTTGGCGCCATCATCATGCCCCACAACAT
  CTACCTGCACTCGGCCCTGGTCAAGTCTCGAGAGATAGACCGGGCCCGCCGAGCGGACATCAGAGAAGCC
  AACATGTACTTCCTGATTGAGGCCACCATCGCCCTGTCCGTCTCCTTTATCATCAACCTCTTTGTCATGG
  CTGTCTTTGGGCAGGCCTTCTACCAGAAAACCAACCAGGCTGCGTTCAACATCTGTGCCAACAGCAGCCT
  CCACGACTACGCCAAGATCTTCCCCATGAACAACGCCACCGTGGCCGTGGACATTTACCAGGGGGGCGTG
  ATCCTGGGCTGCCTGTTCGGCCCCGCGGCCCTCTACATCTGGGCCATAGGTCTCCTGGCGGCTGGGCAGA
  GCTCCACCATGACGGGCACCTACGCGGGACAGTTCGTGATGGAGGGCTTCCTGAGGCTGCGGTGGTCACG
  CTTCGCCCGTGTCCTCCTCACCCGCTCCTGCGCCATCCTGCCCACCGTGCTCGTGGCTGTCTTCCGGGAC
  CTGAGGGACTTGTCGGGCCTCAATGATCTGCTCAACGTGCTGCAGAGCCTGCTGCTCCCGTTCGCCGTGC
  TGCCCATCCTCACGTTCACCAGCATGCCCACCCTCATGCAGGAGTTTGCCAATGGCCTGCTGAACAAGGT
  CGTCACCTCTTCCATCATGGTGCTAGTCTGCGCCATCAACCTCTACTTCGTGGTCAGCTATCTGCCCAGC
  CTGCCCCACCCTGCCTACTTCGGCCTTGCAGCCTTGCTGGCCGCAGCCTACCTGGGCCTCAGCACCTACC
  TGGTCTGGACCTGTTGCCTTGCCCACGGAGCCACCTTTCTGGCCCACAGCTCCCACCACCACTTCCTGTA
  TGGGCTCCTTGAAGAGGACCAGAAAGGGGAGACCTCTGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219733 representing NM_000578
  Red=Cloning site Green=Tags(s)
  
  MTGDKGPQRLSGSSYGSISSPTSPTSPGPRQAPPRETYLSEKIPIPDTKPGTFSLRKLWAFTGPGFLMSI
  AFLDPGNIESDLQAGAVAGFKLLWVLLWATVLGLLCQRLAARLGVVTGKDLGEVCHLYYPKVPRTVLWLT
  IELAIVGSDMQEVIGTAIAFNLLSAGRIPLWGGVLITIVDTFFFLFLDNYGLRKLEAFFGLLITIMALTF
  GYEYVVARPEQGALLRGLFLPSCPGCGHPELLQAVGIVGAIIMPHNIYLHSALVKSREIDRARRADIREA
  NMYFLIEATIALSVSFIINLFVMAVFGQAFYQKTNQAAFNICANSSLHDYAKIFPMNNATVAVDIYQGGV
  ILGCLFGPAALYIWAIGLLAAGQSSTMTGTYAGQFVMEGFLRLRWSRFARVLLTRSCAILPTVLVAVFRD
  LRDLSGLNDLLNVLQSLLLPFAVLPILTFTSMPTLMQEFANGLLNKVVTSSIMVLVCAINLYFVVSYLPS
  LPHPAYFGLAALLAAAYLGLSTYLVWTCCLAHGATFLAHSSHHHFLYGLLEEDQKGETSG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000578
• ORF Size: 1650 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_000578.2, NP_000569.2
• RefSeq Size: 2573
• RefSeq ORF: 1653
• Locus ID: 6556
• Domains: Nramp
• Protein Families: Transmembrane
• Protein Pathways: Lysosome
• Gene Summary: This gene is a member of the solute carrier family 11 (proton-coupled divalent metal ion transporters) family and encodes a multi-pass membrane protein. The protein functions as a divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Mutations in this gene have been associated with susceptibility to infectious diseases such as tuberculosis and leprosy, and inflammatory diseases such as rheumatoid arthritis and Crohn disease. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]