Kv1.4 (KCNA4) (NM_002233) Human Tagged ORF Clone

CAT#: RG220205

KCNA4 (GFP-tagged) - Human potassium voltage-gated channel, shaker-related subfamily, member 4 (KCNA4)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KCNA4
• Synonyms: HBK4; HK1; HPCN2; HUKII; KCNA4L; KCNA8; KV1.4; MCIDDS; PCN2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220205 representing NM_002233
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTTGCAATGGTGAGTGCGGAGAGCTCAGGGTGCAACAGTCACATGCCTTATGGTTATGCTGCCC
  AGGCCCGGGCCCGGGAGCGGGAGAGGCTTGCTCACTCCAGGGCAGCTGCAGCAGCTGCTGTTGCAGCGGC
  CACAGCTGCTGTCGAAGGTAGCGGGGGTTCTGGTGGGGGCTCCCACCACCACCACCAGTCACGCGGGGCC
  TGTACCTCCCATGACCCTCAGAGCAGCCGGGGTAGTCGGAGGAGGAGGCGACAGCGGTCTGAGAAGAAGA
  AAGCCCACTACCGGCAGAGCAGCTTCCCTCATTGCTCTGACCTGATGCCCAGTGGCTCTGAGGAGAAGAT
  CCTGAGGGAGCTGAGTGAGGAGGAGGAAGATGAGGAGGAGGAGGAAGAGGAGGAAGAGGAGGGAAGGTTT
  TACTATAGTGAAGATGACCATGGTGATGAGTGTTCCTACACGGATCTGCTGCCTCAGGATGAGGGCGGTG
  GCGGCTACAGTTCAGTCCGCTACAGTGACTGTTGTGAACGTGTGGTGATAAATGTGTCAGGCCTACGCTT
  TGAGACCCAAATGAAAACTCTGGCCCAGTTTCCAGAGACTTTGTTGGGAGACCCTGAAAAGAGGACTCAG
  TACTTTGACCCTTTGCGCAATGAGTATTTTTTTGACAGGAACCGCCCCAGCTTTGATGCCATCTTGTATT
  ATTATCAATCAGGAGGCCGCCTGAAGAGGCCAGTCAATGTCCCCTTTGATATCTTCACTGAGGAGGTGAA
  GTTCTATCAGTTGGGGGAGGAGGCCCTGTTGAAGTTTCGGGAGGACGAGGGCTTTGTGAGAGAAGAGGAA
  GACAGGGCCCTCCCCGAGAATGAATTTAAAAAGCAGATTTGGCTCCTCTTTGAATATCCAGAGAGCTCCA
  GTCCTGCAAGGGGCATAGCCATTGTGTCCGTCCTGGTCATCTTAATCTCCATTGTCATCTTTTGCCTGGA
  AACCTTGCCTGAGTTTAGGGACGACAGGGATCTCGTCATGGCACTGAGTGCTGGCGGGCATGGTGGGTTG
  TTGAATGATACTTCAGCACCCCATCTGGAGAACTCAGGGCACACAATATTCAATGACCCCTTCTTCATCG
  TGGAAACAGTCTGTATTGTATGGTTTTCCTTTGAGTTTGTGGTTCGCTGCTTTGCTTGTCCCAGCCAAGC
  ACTCTTCTTCAAAAACATCATGAACATCATTGACATTGTCTCCATTTTGCCTTACTTCATCACACTGGGC
  ACTGACCTGGCCCAGCAACAGGGGGGTGGCAATGGTCAGCAGCAGCAGGCCATGTCCTTTGCCATCCTCA
  GAATCATTCGTCTGGTCCGAGTATTCCGGATCTTCAAACTCTCCAGGCACTCCAAAGGCCTGCAGATCCT
  GGGCCACACCCTCAGAGCCAGCATGCGGGAACTGGGCCTTCTGATCTTCTTCCTCTTCATTGGGGTCATC
  CTCTTTTCTAGTGCTGTGTATTTTGCAGAGGCGGATGAACCTACTACCCATTTCCAAAGCATCCCAGATG
  CATTTTGGTGGGCTGTGGTGACCATGACAACTGTGGGCTATGGGGACATGAAGCCCATCACTGTAGGGGG
  CAAGATTGTCGGGTCCCTGTGTGCCATTGCGGGTGTCTTAACCATTGCTTTGCCAGTGCCAGTGATTGTC
  TCTAACTTTAACTATTTCTACCACAGAGAGACTGAAAATGAGGAACAGACACAGCTAACGCAGAATGCAG
  TCAGTTGTCCATACCTCCCCTCTAATTTGCTCAAGAAATTTCGGAGCTCTACTTCTTCTTCCCTGGGGGA
  CAAGTCAGAGTATCTAGAGATGGAAGAAGGAGTTAAGGAATCTCTGTGTGCAAAGGAGGAGAAGTGTCAG
  GGAAAGGGGGATGACAGTGAGACAGATAAAAACAACTGTTCTAATGCAAAGGCTGTGGAGACTGATGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220205 representing NM_002233
  Red=Cloning site Green=Tags(s)
  
  MEVAMVSAESSGCNSHMPYGYAAQARARERERLAHSRAAAAAAVAAATAAVEGSGGSGGGSHHHHQSRGA
  CTSHDPQSSRGSRRRRRQRSEKKKAHYRQSSFPHCSDLMPSGSEEKILRELSEEEEDEEEEEEEEEEGRF
  YYSEDDHGDECSYTDLLPQDEGGGGYSSVRYSDCCERVVINVSGLRFETQMKTLAQFPETLLGDPEKRTQ
  YFDPLRNEYFFDRNRPSFDAILYYYQSGGRLKRPVNVPFDIFTEEVKFYQLGEEALLKFREDEGFVREEE
  DRALPENEFKKQIWLLFEYPESSSPARGIAIVSVLVILISIVIFCLETLPEFRDDRDLVMALSAGGHGGL
  LNDTSAPHLENSGHTIFNDPFFIVETVCIVWFSFEFVVRCFACPSQALFFKNIMNIIDIVSILPYFITLG
  TDLAQQQGGGNGQQQQAMSFAILRIIRLVRVFRIFKLSRHSKGLQILGHTLRASMRELGLLIFFLFIGVI
  LFSSAVYFAEADEPTTHFQSIPDAFWWAVVTMTTVGYGDMKPITVGGKIVGSLCAIAGVLTIALPVPVIV
  SNFNYFYHRETENEEQTQLTQNAVSCPYLPSNLLKKFRSSTSSSLGDKSEYLEMEEGVKESLCAKEEKCQ
  GKGDDSETDKNNCSNAKAVETDV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002233
• ORF Size: 1959 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002233.2, NP_002224.1
• RefSeq Size: 3613 bp
• RefSeq ORF: 1962 bp
• Locus ID: 3739
• Cytogenetics: 11p14.1
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the A-type potassium current class, the members of which may be important in the regulation of the fast repolarizing phase of action potentials in heart and thus may influence the duration of cardiac action potential.[provided by RefSeq, Mar 2011]'