LMO7 (NM_005358) Human Tagged ORF Clone

CAT#: RG220301

LMO7 (GFP-tagged) - Human LIM domain 7 (LMO7), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LMO7
• Synonyms: FBX20; FBXO20; LMO7b; LOMP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220301 representing NM_005358
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAAAATTAGGATATGCCATATTTTCACGTTTTACAGTTGGATGTCCTATGATGTTCTCTTCCAGA
  GAACAGAGCTCGGAGCTCTGGAAATTTGGAGGCAACTGATATGTGCTCATGTCTGCATCTGTGTGGGTTG
  GCTGTATCTCAGGGACAGAGTCTGCAGCAAAAAAGATATAATTTTGAGGACTGAACAAAATTCAGGAAGG
  ACTATTCTCATTAAGGCAGTAACAGAGAAGAATTTTGAAACAAAAGATTTTCGAGCCTCTCTAGAAAATG
  GTGTTCTGCTGTGTGATTTGATTAATAAGCTTAAACCTGGCGTCATTAAGAAGATCAATAGACTGTCTAC
  ACCAATAGCAGGATTGGATAATATAAACGTTTTCTTGAAAGCTTGTGAACAGATTGGATTGAAAGAAGCC
  CAGCTTTTCCATCCTGGAGATCTACAGGATTTATCAAATCGAGTCACTGTCAAGCAAGAAGAGACTGACA
  GGAGAGTGAAAAATGTTTTGATAACATTGTACTGGCTGGGAAGAAAAGCACAAAGCAACCCGTACTATAA
  TGGTCCCCATCTTAATTTGAAAGCGTTTGAGAATCTTTTAGGACAAGCACTGACGAAGGCACTCGAAGAC
  TCCAGCTTCCTGAAAAGAAGTGGCAGGGACAGTGGCTACGGTGACATCTGGTGTCCTGAACGTGGAGAAT
  TTCTTGCTCCTCCAAGGCACCATAAGAGAGAAGATTCCTTTGAAAGCTTGGACTCTTTGGGCTCGAGGTC
  ATTGACAAGCTGCTCCTCTGATATCACGTTGAGAGGGGGGCGTGAAGGTTTTGAAAGTGACACAGATTCG
  GAATTTACATTTAAGATGCAGGATTATAATAAAGATGATATGTCGTATCGAAGGATTTCGGCTGTTGAGC
  CAAAGACTGCGTTACCCTTCAATCGTTTTTTACCCAACAAAAGTAGACAGCCATCCTATGTACCAGCACC
  TCTGAGAAAGAAAAAGCCAGACAAACATGAGGATAACAGAAGAAGTTGGGCAAGCCCGGTTTATACAGAA
  GCAGATGGAACATTTTCAAGACTCTTTCAAAAGATTTATGGTGAGAATGGGAGTAAGTCCATGAGTGATG
  TCAGCGCAGAAGATGTTCAAAACTTGCGTCAGCTGCGTTACGAGGAGATGCAGAAAATAAAATCACAATT
  AAAAGAACAAGATCAGAAATGGCAGGATGACCTTGCAAAATGGAAAGATCGTCGAAAAAGTTACACTTCA
  GATCTGCAGAAGAAAAAAGAAGAGAGAGAAGAAATTGAAAAGCAGGCACTTGAGAAGTCTAAGAGAAGCT
  CTAAGACGTTTAAGGAAATGCTGCAGGACAGGGAATCCCAAAATCAAAAGTCTACAGTTCCGTCAAGAAG
  GAGAATGTATTCTTTTGATGATGTGCTGGAGGAAGGAAAGCGACCCCCTACAATGACTGTGTCAGAAGCA
  AGTTACCAGAGTGAGAGAGTAGAAGAGAAGGGAGCAACTTATCCTTCAGAAATTCCCAAAGAAGATTCTA
  CCACTTTTGCAAAAAGAGAGGACCGTGTAACAACTGAAATTCAGCTTCCTTCTCAAAGTCCTGTGGAAGA
  ACAAAGCCCAGCCTCTTTGTCTTCTCTGCGTTCACGGAGCACACAAATGGAATCAACTCGTGTTTCAGCT
  TCTCTCCCCAGAAGTTACCGGAAAACTGATACAGTCAGGTTAACATCTGTGGTCACACCAAGACCCTTTG
  GCTCTCAGACAAGGGGAATCTCATCACTCCCCAGATCTTACACGATGGATGATGCTTGGAAGTATAATGG
  AGATGTTGAAGACATTAAGAGAACTCCAAACAATGTGGTCAGCACCCCTGCACCAAGCCCGGACGCAAGC
  CAACTGGCTTCAAGCTTATCTAGCCAGAAAGAGGTAGCAGCAACAGAAGAAGATGTGACAAGGCTGCCCT
  CTCCTACATCCCCCTTCTCATCTCTTTCCCAAGACCAGGCTGCCACTTCTAAAGCCACATTGTCTTCCAC
  ATCTGGTCTTGATTTAATGTCTGAATCTGGAGAAGGGGAAATCTCCCCACAAAGAGAAGTCTCAAGATCC
  CAGGATCAGTTCAGTGATATGAGAATCAGCATAAACCAGACGCCTGGGAAGAGTCTTGACTTTGGGTTTA
  CAATAAAATGGGATATTCCTGGGATCTTCGTAGCATCAGTTGAAGCAGGTAGCCCAGCAGAATTTTCTCA
  GCTACAAGTAGATGATGAAATTATTGCTATTAACAACACCAAGTTTTCATATAACGATTCAAAAGAGTGG
  GAGGAAGCCATGGCTAAGGCTCAAGAAACTGGACACCTAGTGATGGATGTGAGGCGCTATGGAAAGGCTG
  GTTCACCTGAAACAAAGTGGATTGATGCAACTTCTGGAATTTACAACTCAGAAAAATCTTCAAATCTATC
  TGTAACAACTGATTTCTCCGAAAGCCTTCAGAGTTCTAATATTGAATCCAAAGAAATCAATGGAATTCAT
  GATGAAAGCAATGCTTTTGAATCAAAAGCATCTGAATCCATTTCTTTGAAAAACTTAAAAAGGCGATCAC
  AATTTTTTGAACAAGGAAGCTCTGATTCGGTGGTTCCTGATCTTCCAGTTCCAACCATCAGTGCCCCGAG
  TCGCTGGGTGTGGGATCAAGAGGAGGAGCGGAAGCGGCAGGAGAGGTGGCAGAAGGAGCAGGACCGCCTA
  CTGCAGGAAAAATATCAACGTGAGCAGGAGAAACTGAGGGAAGAGTGGCAAAGGGCCAAACAGGAGGCAG
  AGAGAGAGAATTCCAAGTACTTGGATGAGGAACTGATGGTCCTAAGCTCAAACAGCATGTCTCTGACCAC
  ACGGGAGCCCTCTCTTGCCACCTGGGAAGCTACCTGGAGTGAAGGGTCCAAGTCTTCAGACAGAGAAGGA
  ACCCGAGCAGGAGAAGAGGAGAGGAGACAGCCACAAGAGGAAGTTGTTCATGAGGACCAAGGAAAGAAGC
  CGCAGGATCAGCTTGTTATTGAGAGAGAGAGGAAATGGGAGCAACAGCTTCAGGAAGAGCAAGAGCAAAA
  GCGGCTTCAGGCTGAGGCTGAGGAGCAGAAGCGTCCTGCGGAGGAGCAGAAGCGCCAGGCAGAGATAGAG
  CGGGAAACATCAGTCAGAATATACCAGTACAGGAGGCCTGTTGATTCCTATGATATACCAAAGACAGAAG
  AAGCATCTTCAGGTTTTCTTCCTGGTGACAGGAATAAATCCAGATCTACTACTGAACTGGATGATTACTC
  CACAAATAAAAATGGAAACAATAAATATTTAGACCAAATTGGGAACATGACCTCTTCACAGAGGAGATCC
  AAGAAAGAACAAGTACCATCAGGAGCAGAATTGGAGAGGCAACAAATCCTTCAGGAAATGAGGAAGAGAA
  CACCCCTTCACAATGACAACAGCTGGATCCGACAGCGCAGTGCCAGTGTCAACAAAGAGCCTGTTAGTCT
  TCCTGGGATCATGAGAAGAGGCGAATCTTTAGATAACCTGGACTCCCCCCGATCCAATTCTTGGAGACAG
  CCTCCTTGGCTCAATCAGCCCACAGGATTCTATGCTTCTTCCTCTGTGCAAGACTTTAGTCGCCCACCAC
  CTCAGCTGGTGTCCACATCAAACCGTGCCTACATGCGGAACCCCTCCTCCAGCGTGCCCCCACCTTCAGC
  TGGCTCCGTGAAGACCTCCACCACAGGTGTGGCCACCACACAGTCCCCCACCCCGAGAAGCCATTCCCCT
  TCAGCTTCACAGTCAGGCTCTCAGCTGCGTAACAGGTCAGTCAGTGGGAAGCGCATATGCTCCTACTGCA
  ATAACATTCTGGGCAAAGGAGCCGCCATGATCATCGAGTCCCTGGGTCTTTGTTATCATTTGCATTGTTT
  TAAGTGTGTTGCCTGTGAGTGTGACCTCGGAGGCTCTTCCTCAGGAGCTGAAGTCAGGATCAGAAACCAC
  CAACTGTACTGCAACGACTGCTATCTCAGATTCAAATCTGGACGGCCAACCGCCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220301 representing NM_005358
  Red=Cloning site Green=Tags(s)
  
  MKKIRICHIFTFYSWMSYDVLFQRTELGALEIWRQLICAHVCICVGWLYLRDRVCSKKDIILRTEQNSGR
  TILIKAVTEKNFETKDFRASLENGVLLCDLINKLKPGVIKKINRLSTPIAGLDNINVFLKACEQIGLKEA
  QLFHPGDLQDLSNRVTVKQEETDRRVKNVLITLYWLGRKAQSNPYYNGPHLNLKAFENLLGQALTKALED
  SSFLKRSGRDSGYGDIWCPERGEFLAPPRHHKREDSFESLDSLGSRSLTSCSSDITLRGGREGFESDTDS
  EFTFKMQDYNKDDMSYRRISAVEPKTALPFNRFLPNKSRQPSYVPAPLRKKKPDKHEDNRRSWASPVYTE
  ADGTFSRLFQKIYGENGSKSMSDVSAEDVQNLRQLRYEEMQKIKSQLKEQDQKWQDDLAKWKDRRKSYTS
  DLQKKKEEREEIEKQALEKSKRSSKTFKEMLQDRESQNQKSTVPSRRRMYSFDDVLEEGKRPPTMTVSEA
  SYQSERVEEKGATYPSEIPKEDSTTFAKREDRVTTEIQLPSQSPVEEQSPASLSSLRSRSTQMESTRVSA
  SLPRSYRKTDTVRLTSVVTPRPFGSQTRGISSLPRSYTMDDAWKYNGDVEDIKRTPNNVVSTPAPSPDAS
  QLASSLSSQKEVAATEEDVTRLPSPTSPFSSLSQDQAATSKATLSSTSGLDLMSESGEGEISPQREVSRS
  QDQFSDMRISINQTPGKSLDFGFTIKWDIPGIFVASVEAGSPAEFSQLQVDDEIIAINNTKFSYNDSKEW
  EEAMAKAQETGHLVMDVRRYGKAGSPETKWIDATSGIYNSEKSSNLSVTTDFSESLQSSNIESKEINGIH
  DESNAFESKASESISLKNLKRRSQFFEQGSSDSVVPDLPVPTISAPSRWVWDQEEERKRQERWQKEQDRL
  LQEKYQREQEKLREEWQRAKQEAERENSKYLDEELMVLSSNSMSLTTREPSLATWEATWSEGSKSSDREG
  TRAGEEERRQPQEEVVHEDQGKKPQDQLVIERERKWEQQLQEEQEQKRLQAEAEEQKRPAEEQKRQAEIE
  RETSVRIYQYRRPVDSYDIPKTEEASSGFLPGDRNKSRSTTELDDYSTNKNGNNKYLDQIGNMTSSQRRS
  KKEQVPSGAELERQQILQEMRKRTPLHNDNSWIRQRSASVNKEPVSLPGIMRRGESLDNLDSPRSNSWRQ
  PPWLNQPTGFYASSSVQDFSRPPPQLVSTSNRAYMRNPSSSVPPPSAGSVKTSTTGVATTQSPTPRSHSP
  SASQSGSQLRNRSVSGKRICSYCNNILGKGAAMIIESLGLCYHLHCFKCVACECDLGGSSSGAEVRIRNH
  QLYCNDCYLRFKSGRPTAM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005358
• ORF Size: 4047 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005358.4, NP_005349.3
• RefSeq Size: 7235 bp
• RefSeq ORF: 4050 bp
• Locus ID: 4008
• Cytogenetics: 13q22.2
• Domains: PDZ, LIM
• Protein Pathways: Adherens junction
• Gene Summary: 'This gene encodes a protein containing a calponin homology (CH) domain, a PDZ domain, and a LIM domain, and may be involved in protein-protein interactions. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene, however, the full-length nature of some variants is not known. [provided by RefSeq, Jan 2009]'