Angiomotin (AMOT) (NM_133265) Human Tagged ORF Clone

CAT#: RG220347

AMOT (GFP-tagged) - Human angiomotin (AMOT), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AMOT
• Synonyms: KIAA1071
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220347 representing NM_133265
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTCGGGCTCAGCCATCCTCTGCTTCTTATCAGCCAGTGCCAGCAGACCCTTTTGCCATTGTTTCCA
  GAGCCCAGCAGATGGTTGAGATCCTCTCAGACGAGAACCGGAACTTGAGGCAAGAGTTGGAAGGATGCTA
  TGAGAAGGTGGCAAGACTGCAGAAGGTGGAGACAGAAATCCAGCGCGTCTCGGAGGCATATGAGAACCTC
  GTGAAGTCATCCTCCAAAAGAGAGGCCCTAGAGAAAGCCATGAGAAACAAGCTAGAGGGCGAGATTCGGA
  GGATGCATGATTTCAACAGGGATCTGAGAGAGCGTCTAGAGACTGCCAACAAGCAGCTTGCAGAGAAGGA
  ATATGAGGGGTCAGAGGACACCAGAAAAACCATCTCGCAGCTCTTTGCAAAAAATAAAGAAAGCCAGCGT
  GAGAAGGAGAAGCTGGAAGCGGAGCTGGCCACTGCCCGTTCTACCAATGAGGACCAAAGACGACACATCG
  AAATCCGAGATCAGGCCCTGAGTAATGCCCAGGCCAAGGTGGTAAAGCTGGAAGAAGAGCTGAAAAAGAA
  GCAAGTGTACGTTGACAAGGTGGAGAAGATGCAGCAGGCCCTTGTACAGCTCCAGGCAGCATGTGAAAAA
  CGTGAGCAGCTAGAGCACCGTCTCCGGACACGACTGGAGAGGGAACTGGAATCCCTGAGAATCCAGCAGC
  GTCAGGGCAACTGTCAGCCCACCAACGTTTCAGAATACAATGCTGCTGCACTGATGGAGCTCCTTCGGGA
  GAAAGAGGAGAGGATTCTGGCTCTGGAAGCTGATATGACAAAGTGGGAGCAGAAATATTTGGAGGAGAAT
  GTGATGAGACATTTTGCTCTGGATGCTGCTGCAACTGTGGCTGCTCAGAGGGACACAACAGTCATCAGTC
  ACTCTCCTAACACCAGCTATGACACAGCTCTAGAAGCTCGCATCCAGAAAGAGGAGGAAGAAATCTTGAT
  GGCCAATAAGCGTTGCCTTGACATGGAGGGCAGGATTAAGACCCTCCATGCCCAGATTATTGAGAAGGAT
  GCCATGATCAAAGTACTCCAGCAGCGTTCCCGGAAGGAGCCGAGCAAGACAGAGCAGCTGTCGTGCATGC
  GGCCAGCGAAGTCTCTGATGTCCATTTCCAATGCTGGATCAGGCTTGCTCTCCCACTCATCCACCCTGAC
  TGGCTCCCCCATCATGGAAGAAAAGCGAGACGACAAGAGCTGGAAGGGGAGCCTAGGCATTCTCCTGGGT
  GGAGACTACCGTGCTGAATATGTCCCTTCCACACCCTCGCCTGTGCCACCCTCGACTCCCCTGCTCTCGG
  CTCACTCCAAGACAGGCAGCCGAGACTGCAGTACCCAAACTGAACGTGGGACGGAATCGAACAAAACTGC
  AGCTGTTGCTCCCATCTCTGTTCCTGCTCCAGTTGCTGCTGCCGCCACTGCTGCCGCCATCACTGCCACT
  GCTGCCACCATCACCACCACCATGGTAGCTGCTGCTCCAGTTGCTGTTGCTGCTGCTGCTGCTCCAGCTG
  CTGCTGCTGCCCCGTCTCCAGCCACTGCCGCTGCTACTGCTGCTGCTGTTTCTCCAGCTGCTGCTGGTCA
  GATTCCAGCTGCTGCCTCTGTTGCCTCAGCTGCTGCCGTTGCTCCTTCTGCTGCTGCTGCTGCTGCTGTT
  CAGGTTGCTCCAGCTGCTCCGGCTCCAGTTCCAGCTCCGGCTCTGGTTCCGGTTCCAGCTCCAGCAGCGG
  CTCAGGCTTCTGCTCCTGCTCAGACTCAGGCACCAACTTCAGCTCCGGCTGTGGCTCCAACTCCAGCTCC
  AACTCCAACTCCAGCTGTGGCTCAGGCTGAGGTTCCTCCTGCAAGTCCAGCTACCGGTCCTGGACCACAT
  CGTTTGTCTATACCAAGTTTGACCTGCAATCCAGACAAAACAGATGGGCCTGTGTTCCACTCCAATACTC
  TGGAAAGAAAAACTCCCATTCAGATCCTGGGACAAGAGCCTGATGCAGAGATGGTGGAATATCTCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220347 representing NM_133265
  Red=Cloning site Green=Tags(s)
  
  MPRAQPSSASYQPVPADPFAIVSRAQQMVEILSDENRNLRQELEGCYEKVARLQKVETEIQRVSEAYENL
  VKSSSKREALEKAMRNKLEGEIRRMHDFNRDLRERLETANKQLAEKEYEGSEDTRKTISQLFAKNKESQR
  EKEKLEAELATARSTNEDQRRHIEIRDQALSNAQAKVVKLEEELKKKQVYVDKVEKMQQALVQLQAACEK
  REQLEHRLRTRLERELESLRIQQRQGNCQPTNVSEYNAAALMELLREKEERILALEADMTKWEQKYLEEN
  VMRHFALDAAATVAAQRDTTVISHSPNTSYDTALEARIQKEEEEILMANKRCLDMEGRIKTLHAQIIEKD
  AMIKVLQQRSRKEPSKTEQLSCMRPAKSLMSISNAGSGLLSHSSTLTGSPIMEEKRDDKSWKGSLGILLG
  GDYRAEYVPSTPSPVPPSTPLLSAHSKTGSRDCSTQTERGTESNKTAAVAPISVPAPVAAAATAAAITAT
  AATITTTMVAAAPVAVAAAAAPAAAAAPSPATAAATAAAVSPAAAGQIPAAASVASAAAVAPSAAAAAAV
  QVAPAAPAPVPAPALVPVPAPAAAQASAPAQTQAPTSAPAVAPTPAPTPTPAVAQAEVPPASPATGPGPH
  RLSIPSLTCNPDKTDGPVFHSNTLERKTPIQILGQEPDAEMVEYLI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133265
• ORF Size: 2028 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133265.2, NP_573572.1
• RefSeq Size: 6514
• RefSeq ORF: 2028
• Locus ID: 154796
• Gene Summary: This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]