ZNF274 (NM_016325) Human Tagged ORF Clone

CAT#: RG220631

ZNF274 (GFP-tagged) - Human zinc finger protein 274 (ZNF274), transcript variant ZNF274a

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF274
• Synonyms: HFB101; ZF2; ZKSCAN19; ZSCAN51
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220631 representing NM_016325
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCAGGCTTCCGACGGCCTGGTCCTGTGAACCAGTGACCTTTGAAGATGTAACACTGGGTTTTA
  CCCCGGAAGAGTGGGGACTGCTGGACCTCAAACAGAAGTCCCTGTACAGGGAAGTGATGCTGGAGAACTA
  CAGGAACCTGGTCTCAGTGGAGTATCCTGAGCTCCAGCTGGACCCTAAATTGGATCCTCTTCCTGCTGAG
  AGTCCCCTAATGAACATTGAGGTTGTTGAGGTCCTCACACTGAACCAGGAGGTGGCTGGTCCCCGGAATG
  CCCAGATCCAGGCCCTATATGCTGAAGATGGAAGCCTGAGTGCAGATGCCCCCAGTGAGCAGGTCCAACA
  GCAGGGCAAGCATCCAGGTGACCCTGAGGCCGCGCGCCAGAGGTTCCGGCAGTTCCGTTATAAGGACATG
  ACAGGTCCCCGGGAGGCCCTGGACCAGCTCCGAGAGCTGTGTCACCAGTGGCTACAGCCTAAGGCACGCT
  CCAAGGAGCAGATCCTGGAGCTGCTGGTGCTGGAGCAGTTCCTAGGTGCACTGCCTGTGAAGCTCCGGAC
  ATGGGTGGAATCGCAGCACCCAGAGAACTGCCAAGAGGTGGTGGCCCTGGTAGAGGGTGTGACCTGGATG
  TCTGAGGAGGAAGTACTTCCTGCAGGACAACCTGCCGAGGGCACCACCTGCTGCCTCGAGGTCACTGCCC
  AGCAGGAGGAGAAGCAGGAGGATGCAGCCATCTGCCCAGTGACAGTGCTCCCTGAGGAGCCAGTGACCTT
  CCAGGATGTGGCTGTGGACTTCAGCCGGGAGGAGTGGGGGCTGCTGGGCCCGACACAGAGGACCGAGTAC
  CGCGATGTGATGCTGGAGACCTTTGGGCACCTGGTCTCTGTGGGGTGGGAGACTACACTGGAAAATAAAG
  AGTTAGCTCCAAATTCTGACATTCCTGAGGAAGAACCAGCCCCCAGCCTGAAAGTACAAGAATCCTCAAG
  GGATTGTGCCTTGTCCTCTACATTAGAAGATACCTTGCAGGGTGGGGTCCAGGAAGTCCAAGACACAGTG
  TTGAAGCAGATGGAGTCTGCTCAGGAAAAAGACCTTCCTCAGAAGAAGCACTTTGACAACCGTGAGTCCC
  AGGCAAACAGTGGTGCTCTTGACACAAACCAAGTTTCGCTCCAGAAAATTGACAACCCTGAGTCCCAGGC
  AAACAGTGGCGCTCTTGACACAAACCAAGTTTTGCTCCACAAAATTCCTCCTAGAAAACGATTGCGCAAA
  CGTGACTCACAAGTTAAAAGTATGAAACATAATTCACGTGTAAAAATTCATCAGAAGAGCTGTGAAAGGC
  AAAAGGCCAAGGAAGGCAATGGTTGTAGGAAAACCTTCAGTCGGAGTACTAAACAGATTACGTTTATAAG
  AATTCACAAGGGGAGCCAAGTTTGCCGATGCAGTGAATGTGGTAAAATATTCCGGAACCCAAGATACTTT
  TCTGTGCATAAGAAAATCCATACCGGAGAGAGGCCCTATGTGTGTCAAGACTGTGGGAAAGGATTTGTTC
  AGAGCTCTTCCCTCACACAGCATCAGAGAGTTCATTCTGGAGAGAGACCATTTGAATGTCAGGAGTGTGG
  GAGGACCTTCAATGATCGCTCAGCCATCTCCCAGCACCTGAGGACTCACACTGGCGCTAAGCCCTACAAG
  TGTCAGGACTGTGGAAAAGCCTTCCGCCAGAGCTCCCACCTCATCAGACATCAGAGGACTCACACCGGGG
  AGCGCCCATATGCATGCAACAAATGTGGAAAGGCCTTCACCCAGAGCTCACACCTTATTGGGCACCAGAG
  AACCCACAATAGGACAAAGCGAAAGAAGAAACAGCCTACCTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220631 representing NM_016325
  Red=Cloning site Green=Tags(s)
  
  MASRLPTAWSCEPVTFEDVTLGFTPEEWGLLDLKQKSLYREVMLENYRNLVSVEYPELQLDPKLDPLPAE
  SPLMNIEVVEVLTLNQEVAGPRNAQIQALYAEDGSLSADAPSEQVQQQGKHPGDPEAARQRFRQFRYKDM
  TGPREALDQLRELCHQWLQPKARSKEQILELLVLEQFLGALPVKLRTWVESQHPENCQEVVALVEGVTWM
  SEEEVLPAGQPAEGTTCCLEVTAQQEEKQEDAAICPVTVLPEEPVTFQDVAVDFSREEWGLLGPTQRTEY
  RDVMLETFGHLVSVGWETTLENKELAPNSDIPEEEPAPSLKVQESSRDCALSSTLEDTLQGGVQEVQDTV
  LKQMESAQEKDLPQKKHFDNRESQANSGALDTNQVSLQKIDNPESQANSGALDTNQVLLHKIPPRKRLRK
  RDSQVKSMKHNSRVKIHQKSCERQKAKEGNGCRKTFSRSTKQITFIRIHKGSQVCRCSECGKIFRNPRYF
  SVHKKIHTGERPYVCQDCGKGFVQSSSLTQHQRVHSGERPFECQECGRTFNDRSAISQHLRTHTGAKPYK
  CQDCGKAFRQSSHLIRHQRTHTGERPYACNKCGKAFTQSSHLIGHQRTHNRTKRKKKQPTS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016325
• ORF Size: 1863 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016325.2, NP_057409.1
• RefSeq Size: 2743
• RefSeq ORF: 1866
• Locus ID: 10782
• Protein Families: Transcription Factors
• Protein Pathways: Neurotrophin signaling pathway
• Gene Summary: This gene encodes a zinc finger protein containing five C2H2-type zinc finger domains, one or two Kruppel-associated box A (KRAB A) domains, and a leucine-rich domain. The encoded protein has been suggested to be a transcriptional repressor. It localizes predominantly to the nucleolus. Alternatively spliced transcript variants encoding different isoforms exist. These variants utilize alternative polyadenylation signals. [provided by RefSeq, Jul 2008]