CYP8B1 (NM_004391) Human Tagged ORF Clone

CAT#: RG220836

CYP8B1 (GFP-tagged) - Human cytochrome P450, family 8, subfamily B, polypeptide 1 (CYP8B1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CYP8B1
• Synonyms: CP8B; CYP12
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220836 representing NM_004391
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCTCTGGGGTCCAGTGCTGGGAGCTCTGCTGGTGGTCATTGCTGGATACCTGTGCCTGCCAGGGA
  TGCTCCGACAACGCAGGCCATGGGAGCCCCCTCTGGACAAGGGTACCGTGCCCTGGCTTGGCCATGCCAT
  GGCTTTCCGGAAGAATATGTTTGAATTTCTGAAGCGCATGAGGACCAAGCATGGGGATGTGTTCACAGTG
  CAGCTAGGGGGCCAGTACTTCACCTTCGTCATGGACCCCCTCTCCTTTGGCCCCATCCTCAAGGACACAC
  AGAGAAAACTAGACTTTGGGCAATATGCAAAAAAACTGGTGCTGAAGGTATTTGGATACCGTTCAGTGCA
  AGGGGACCATGAGATGATACACTCAGCCAGCACCAAGTATCTGAGGGGGGATGGCTTGAAGGATCTTAAT
  GAGACCATGCTGGACAGCCTGTCCTTTGTAATGCTGACGTCCAAAGGCTGGAGTCTGGATGCCAGTTGCT
  GGCATGAGGACAGCCTCTTTCGCTTCTGCTATTACATCTTGTTCACAGCTGGCTACCTGAGCTTGTTCGG
  CTACACGAAGGACAAGGAGCAGGACCTGCTACAGGCAGGAGAGTTATTCATGGAGTTCCGCAAGTTTGAC
  CTTCTTTTCCCAAGGTTTGTCTACTCCCTGCTGTGGCCCCGGGAGTGGCTAGAAGTGGGCCGACTCCAGC
  GTCTCTTTCACAAGATGCTCTCCGTGAGCCACAGCCAGGAGAAGGAGGGCATCAGCAACTGGCTGGGCAA
  CATGCTTCAGTTTCTGAGGGAGCAGGGGGTACCCTCAGCTATGCAGGACAAGTTCAACTTCATGATGCTC
  TGGGCCTCCCAGGGGAACACGGGGCCTACCTCTTTCTGGGCCCTCTTGTACCTCCTGAAGCACCCAGAAG
  CTATTCGGGCTGTGAGGGAGGAAGCTACCCAGGTCCTGGGTGAGGCCAGGCTGGAGACCAAGCAGTCCTT
  TGCCTTCAAACTCGGTGCCCTGCAACACACCCCAGTTCTAGACAGCGTGGTGGAGGAGACGCTGCGGCTG
  AGGGCTGCACCCACCCTCCTCAGGTTGGTTCATGAAGACTATACCCTGAAGATGTCCAGTGGGCAGGAGT
  ATCTGTTCCGCCATGGAGACATCCTGGCCCTCTTTCCCTACCTCTCAGTGCACATGGACCCTGACATCCA
  CCCTGAGCCCACCGTCTTCAAGTACGATCGCTTCCTCAACCCTAATGGCAGCCGGAAAGTGGACTTCTTC
  AAGACAGGCAAGAAGATCCACCACTACACCATGCCCTGGGGTTCGGGCGTTTCCATCTGCCCTGGGAGGT
  TCTTTGCACTCAGTGAGGTGAAGCTCTTTATCCTGCTTATGGTCACACACTTTGACTTAGAGTTGGTGGA
  CCCTGACACACCACTACCCCATGTTGACCCGCAGCGCTGGGGTTTTGGCACCATGCAGCCCAGCCACGAT
  GTGCGCTTCCGCTACCGCCTGCATCCTACAGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220836 representing NM_004391
  Red=Cloning site Green=Tags(s)
  
  MVLWGPVLGALLVVIAGYLCLPGMLRQRRPWEPPLDKGTVPWLGHAMAFRKNMFEFLKRMRTKHGDVFTV
  QLGGQYFTFVMDPLSFGPILKDTQRKLDFGQYAKKLVLKVFGYRSVQGDHEMIHSASTKYLRGDGLKDLN
  ETMLDSLSFVMLTSKGWSLDASCWHEDSLFRFCYYILFTAGYLSLFGYTKDKEQDLLQAGELFMEFRKFD
  LLFPRFVYSLLWPREWLEVGRLQRLFHKMLSVSHSQEKEGISNWLGNMLQFLREQGVPSAMQDKFNFMML
  WASQGNTGPTSFWALLYLLKHPEAIRAVREEATQVLGEARLETKQSFAFKLGALQHTPVLDSVVEETLRL
  RAAPTLLRLVHEDYTLKMSSGQEYLFRHGDILALFPYLSVHMDPDIHPEPTVFKYDRFLNPNGSRKVDFF
  KTGKKIHHYTMPWGSGVSICPGRFFALSEVKLFILLMVTHFDLELVDPDTPLPHVDPQRWGFGTMQPSHD
  VRFRYRLHPTE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004391
• ORF Size: 1503 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004391.1, NP_004382.1
• RefSeq Size: 3951 bp
• RefSeq ORF: 1506 bp
• Locus ID: 1582
• Cytogenetics: 3p22.1
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008]'