CYP8B1 (NM_004391) Human Untagged Clone

CAT#: SC108802

CYP8B1 (untagged)-Human cytochrome P450, family 8, subfamily B, polypeptide 1 (CYP8B1)


  "NM_004391" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "CYP8B1"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol CYP8B1
Synonyms CP8B; CYP12
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_004391 edited
CGAGGCAAGGTCCAGGTGCTCAGACCTTTACATCCTGGACTTTCCAGGCCTCCCAAAGCT
CTCTGGCACCCAGGGAACAGTGTGCGTGTCGAGAGCTTAATCCGCAGGAGCATAGCCATG
GTTCTCTGGGGTCCAGTGCTGGGAGCTCTGCTGGTGGTCATTGCTGGATACCTGTGCCTG
CCAGGGATGCTCCGACAACGCAGGCCATGGGAGCCCCCTCTGGACAAGGGTACCGTGCCC
TGGCTTGGCCATGCCATGGCTTTCCGGAAGAATATGTTTGAATTTCTGAAGCGCATGAGG
ACCAAGCATGGGGATGTGTTCACAGTGCAGCTAGGGGGCCAGTACTTCACCTTCGTCATG
GACCCCCTCTCCTTTGGCCCCATCCTCAAGGACACACAGAGAAAACTAGACTTTGGGCAA
TATGCAAAAAAACTGGTGCTGAAGGTATTTGGATACCGTTCAGTGCAAGGGGACCATGAG
ATGATACACTCAGCCAGCACCAAGTATCTGAGGGGGGATGGCTTGAAGGATCTTAATGAG
ACCATGCTGGACAGCCTGTCCTTTGTAATGCTGACGTCCAAAGGCTGGAGTCTGGATGCC
AGTTGCTGGCATGAGGACAGCCTCTTTCGCTTCTGCTATTACATCTTGTTCACAGCTGGC
TACCTGAGCTTGTTCGGCTACACGAAGGACAAGGAGCAGGACCTGCTACAGGCAGGAGAG
TTATTCATGGAGTTCCGCAAGTTTGACCTTCTTTTCCCAAGGTTTGTCTACTCCCTGCTG
TGGCCCCGGGAGTGGCTAGAAGTGGGCCGACTCCAGCGTCTCTTTCACAAGATGCTCTCC
GTGAGCCACAGCCAGGAGAAGGAGGGCATCAGCAACTGGCTGGGCAACATGCTTCAGTTT
CTGAGGGAGCAGGGGGTACCCTCAGCTATGCAGGACAAGTTCAACTTCATGATGCTCTGG
GCCTCCCAGGGGAACACGGGGCCTACCTCTTTCTGGGCCCTCTTGTACCTCCTGAAGCAC
CCAGAAGCTATTCGGGCTGTGAGGGAGGAAGCTACCCAGGTCCTGGGTGAGGCCAGGCTG
GAGACCAAGCAGTCCTTTGCCTTCAAACTCGGTGCCCTGCAACACACCCCAGTTCTAGAC
AGCGTGGTGGAGGAGACGCTGCGGCTGAGGGCTGCACCCACCCTCCTCAGGTTGGTTCAT
GAAGACTATACCCTGAAGATGTCCAGTGGGCAGGAGTATCTGTTCCGCCATGGAGACATC
CTGGCCCTCTTTCCCTACCTCTCAGTGCACATGGACCCTGACATCCACCCTGAGCCCACC
GTCTTCAAGTACGATCGCTTCCTCAACCCTAATGGCAGCCGGAAAGTGGACTTCTTCAAG
ACAGGCAAGAAGATCCACCACTACACCATGCCCTGGGGTTCGGGCGTTTCCATCTGCCCT
GGGAGGTTCTTTGCACTCAGTGAGGTGAAGCTCTTTATCCTGCTTATGGTCACACACTTT
GACTTAGAGTTGGTGGACCCTGACACACCACTACCCCATGTTGACCCGCAGCGCTGGGGT
TTTGGCACCATGCAGCCCAGCCACGATGTGCGCTTCCGCTACCGCCTGCATCCTACAGAG
TGAGCTTGGCCAAGCCAGCTGCAAACCTGGCCAGAGGAGTTCTATTGCATCTCTCACCTG
TTCTCACCCCTCTGCAGCCCCAAGACCCCACTGGCCACCCCTCCCTCTGGTCCTGTGGCA
CCCCCTACCTCTGTTCTGCCTGTCCTCGCTCTCTCCCCGCCTAGTCATCTGACAGGCTTA
TCATTCTCTTTAAAATACCATCTCT
>OriGene 5' read for NM_004391 unedited
GGTTCCCATTAGTATACGATTCCTATAGGGCGGCCGCGAATTCGCACGAGGCAAGGTCCA
GGTGCTCAGACCTTTACATCCTGGACTTTCCAGGCCTCCCAAAGCTCTCTGGCACCCAGG
GAACAGTGTGCGTGTCGAGAGCTTAATCCGCAGGAGCATAGCCATGGTTCTCTGGGGTCC
AGTGCTGGGAGCTCTGCTGGTGGTCATTGCTGGATACCTGTGCCTGCCAGGGATGCTCCG
ACAACGCAGGCCATGGGAGCCCCCTCTGGACAAGGGTACCGTGCCCTGGCTTGGCCATGC
CATGGCTTTCCGGAAGAATATGTTTGAATTTCTGAAGCGCATGAGGACCAAGCATGGGGA
TGTGTTCACAGTGCAGCTAGGGGGCCAGTACTTCACCTTCGTCATGGACCCCCTCTCCTT
TGGCCCCATCCTCAAGGACACACAGAGAAAACTAGACTTTGGGCAATATGCAAAAAAACT
GGTGCTGAAGGTATTTGGATACCGTTCAGTGCAAGGGGACCATGAGATGATACACTCAGC
CAGCACCAAGTATCTGAGGGGGGATGGCTTGAAGGATCTTAATGAGACCATGCTGGACAG
CCTGTCCTTTGTAATGCTGACGTCCAAAGGCTGGAGTCTGGATGCCAGTTGCTGGCATGA
GGACAGCCTCTTTCGCTTCTGCTATTACATCTTGTTCACAGCTGGCTACCTGAGCTTGGT
CGGCTACACCAAGGACAAGGAGCAGGACCTGCTTCCAGTCAGGATAGTTATTCCTTGGAG
TTCCGCAAGTTTGACCTTCTTTTCCAAAGATTGGCTACCCCCTGCTGTGGCCCTCGGAGG
GCTCTTAGTGGCCCGCCTCCCACGTCTTTTCACAGAAGCTCCTCCGGAGCCCCATCCCGA
AAAGGGGCC
>OriGene 3' read for NM_004391 unedited
GGCATTGGGTGACATTTATCCTCCTTTTAGGGAATGGNGGTCAGAATGAATAAAAACCAT
GCCCCATTTGGCTCCAGTCTAGGGAAGTGGGCAGCAGGCAGAAACCAAGACACAGAAAGC
AACGACACATAACAGATATGCTATGATACAAATGGTGCTGCAGGAGCTGCACAGTTCGGA
GGCCATTTCTGGCCAGAGGATGGGATGCGTGAAATCCAGGAAGGCTTCCAGGAAGGAGTA
GCTGGCACTGGGATGGGCAGGAACAGCAATGTCTCTACTGATGGTTTCATTGGAGAAAGC
TGGCAAAGTTGAGAAGTTTGGAGACTAACCGTGACAAAATTTAAACATCAGGTATGTGAA
GGCAAGGGGGACCAACTGTGACAAAATGTAAACGTCAAGTGTGTGAAGTCAAAGGGGAAC
CAGGAAAGTAAAAGAGTGACAATCAGCAGTGCTTTTGGCAAAAAGGCCTCTGGCAGCAGT
GTAGAAGAGAAACGCAGAGATCACAGGTATAAGAATGACAATAGGAGAGGGGAGGAGGGT
ACATATTTCAGAGGCATCACAGGGGGGTGTACTGATAGGACGGGGGCCTGCTTGTATGAA
GGTGAAAGAGGAGGAGGGAGAGTGTCTCTGGGAGATGGGGTGAATGGTAGTGTTCTGAGC
AGAGAGAGCAGGGAAATCANGAGGAGGAGTGGTTCAGGGAAGAAGCAGGGGTTCAGATGC
CAAGTTCTGCATTGGCCAAGCTGAGTCAGAAGTGCTGGGAGACTCCCATGTGGGTCTGGN
GATAGAAAGAAGCTCTGAGTTCAGCAATGATCATGGAAACCCAAGGGNAGGTTTGACAGC
ACCCTGAGGTGGGGGGAGACATGGCACTNGAGAGGNATCTTGNATGATNCCTACATTAAA
GACATNCTGACTGGGCACAGTGGCTCATGCCTGTAATCCCAGAAACTTGG
Restriction Sites NotI-NotI     
ACCN NM_004391
Insert Size 4130 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004391.1, NP_004382.1
RefSeq Size 3951 bp
RefSeq ORF 1506 bp
Locus ID 1582
Cytogenetics 3p22.1
Domains p450
Protein Families Druggable Genome, P450, Transmembrane
Protein Pathways Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis
Gene Summary 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008]'

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.