CYP8B1 (NM_004391) Human Untagged Clone

CAT#: SC108802

CYP8B1 (untagged)-Human cytochrome P450, family 8, subfamily B, polypeptide 1 (CYP8B1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYP8B1
• Synonyms: CP8B; CYP12
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_004391 edited
  CGAGGCAAGGTCCAGGTGCTCAGACCTTTACATCCTGGACTTTCCAGGCCTCCCAAAGCT
  CTCTGGCACCCAGGGAACAGTGTGCGTGTCGAGAGCTTAATCCGCAGGAGCATAGCCATG
  GTTCTCTGGGGTCCAGTGCTGGGAGCTCTGCTGGTGGTCATTGCTGGATACCTGTGCCTG
  CCAGGGATGCTCCGACAACGCAGGCCATGGGAGCCCCCTCTGGACAAGGGTACCGTGCCC
  TGGCTTGGCCATGCCATGGCTTTCCGGAAGAATATGTTTGAATTTCTGAAGCGCATGAGG
  ACCAAGCATGGGGATGTGTTCACAGTGCAGCTAGGGGGCCAGTACTTCACCTTCGTCATG
  GACCCCCTCTCCTTTGGCCCCATCCTCAAGGACACACAGAGAAAACTAGACTTTGGGCAA
  TATGCAAAAAAACTGGTGCTGAAGGTATTTGGATACCGTTCAGTGCAAGGGGACCATGAG
  ATGATACACTCAGCCAGCACCAAGTATCTGAGGGGGGATGGCTTGAAGGATCTTAATGAG
  ACCATGCTGGACAGCCTGTCCTTTGTAATGCTGACGTCCAAAGGCTGGAGTCTGGATGCC
  AGTTGCTGGCATGAGGACAGCCTCTTTCGCTTCTGCTATTACATCTTGTTCACAGCTGGC
  TACCTGAGCTTGTTCGGCTACACGAAGGACAAGGAGCAGGACCTGCTACAGGCAGGAGAG
  TTATTCATGGAGTTCCGCAAGTTTGACCTTCTTTTCCCAAGGTTTGTCTACTCCCTGCTG
  TGGCCCCGGGAGTGGCTAGAAGTGGGCCGACTCCAGCGTCTCTTTCACAAGATGCTCTCC
  GTGAGCCACAGCCAGGAGAAGGAGGGCATCAGCAACTGGCTGGGCAACATGCTTCAGTTT
  CTGAGGGAGCAGGGGGTACCCTCAGCTATGCAGGACAAGTTCAACTTCATGATGCTCTGG
  GCCTCCCAGGGGAACACGGGGCCTACCTCTTTCTGGGCCCTCTTGTACCTCCTGAAGCAC
  CCAGAAGCTATTCGGGCTGTGAGGGAGGAAGCTACCCAGGTCCTGGGTGAGGCCAGGCTG
  GAGACCAAGCAGTCCTTTGCCTTCAAACTCGGTGCCCTGCAACACACCCCAGTTCTAGAC
  AGCGTGGTGGAGGAGACGCTGCGGCTGAGGGCTGCACCCACCCTCCTCAGGTTGGTTCAT
  GAAGACTATACCCTGAAGATGTCCAGTGGGCAGGAGTATCTGTTCCGCCATGGAGACATC
  CTGGCCCTCTTTCCCTACCTCTCAGTGCACATGGACCCTGACATCCACCCTGAGCCCACC
  GTCTTCAAGTACGATCGCTTCCTCAACCCTAATGGCAGCCGGAAAGTGGACTTCTTCAAG
  ACAGGCAAGAAGATCCACCACTACACCATGCCCTGGGGTTCGGGCGTTTCCATCTGCCCT
  GGGAGGTTCTTTGCACTCAGTGAGGTGAAGCTCTTTATCCTGCTTATGGTCACACACTTT
  GACTTAGAGTTGGTGGACCCTGACACACCACTACCCCATGTTGACCCGCAGCGCTGGGGT
  TTTGGCACCATGCAGCCCAGCCACGATGTGCGCTTCCGCTACCGCCTGCATCCTACAGAG
  TGAGCTTGGCCAAGCCAGCTGCAAACCTGGCCAGAGGAGTTCTATTGCATCTCTCACCTG
  TTCTCACCCCTCTGCAGCCCCAAGACCCCACTGGCCACCCCTCCCTCTGGTCCTGTGGCA
  CCCCCTACCTCTGTTCTGCCTGTCCTCGCTCTCTCCCCGCCTAGTCATCTGACAGGCTTA
  TCATTCTCTTTAAAATACCATCTCT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004391 unedited
  GGTTCCCATTAGTATACGATTCCTATAGGGCGGCCGCGAATTCGCACGAGGCAAGGTCCA
  GGTGCTCAGACCTTTACATCCTGGACTTTCCAGGCCTCCCAAAGCTCTCTGGCACCCAGG
  GAACAGTGTGCGTGTCGAGAGCTTAATCCGCAGGAGCATAGCCATGGTTCTCTGGGGTCC
  AGTGCTGGGAGCTCTGCTGGTGGTCATTGCTGGATACCTGTGCCTGCCAGGGATGCTCCG
  ACAACGCAGGCCATGGGAGCCCCCTCTGGACAAGGGTACCGTGCCCTGGCTTGGCCATGC
  CATGGCTTTCCGGAAGAATATGTTTGAATTTCTGAAGCGCATGAGGACCAAGCATGGGGA
  TGTGTTCACAGTGCAGCTAGGGGGCCAGTACTTCACCTTCGTCATGGACCCCCTCTCCTT
  TGGCCCCATCCTCAAGGACACACAGAGAAAACTAGACTTTGGGCAATATGCAAAAAAACT
  GGTGCTGAAGGTATTTGGATACCGTTCAGTGCAAGGGGACCATGAGATGATACACTCAGC
  CAGCACCAAGTATCTGAGGGGGGATGGCTTGAAGGATCTTAATGAGACCATGCTGGACAG
  CCTGTCCTTTGTAATGCTGACGTCCAAAGGCTGGAGTCTGGATGCCAGTTGCTGGCATGA
  GGACAGCCTCTTTCGCTTCTGCTATTACATCTTGTTCACAGCTGGCTACCTGAGCTTGGT
  CGGCTACACCAAGGACAAGGAGCAGGACCTGCTTCCAGTCAGGATAGTTATTCCTTGGAG
  TTCCGCAAGTTTGACCTTCTTTTCCAAAGATTGGCTACCCCCTGCTGTGGCCCTCGGAGG
  GCTCTTAGTGGCCCGCCTCCCACGTCTTTTCACAGAAGCTCCTCCGGAGCCCCATCCCGA
  AAAGGGGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004391 unedited
  GGCATTGGGTGACATTTATCCTCCTTTTAGGGAATGGNGGTCAGAATGAATAAAAACCAT
  GCCCCATTTGGCTCCAGTCTAGGGAAGTGGGCAGCAGGCAGAAACCAAGACACAGAAAGC
  AACGACACATAACAGATATGCTATGATACAAATGGTGCTGCAGGAGCTGCACAGTTCGGA
  GGCCATTTCTGGCCAGAGGATGGGATGCGTGAAATCCAGGAAGGCTTCCAGGAAGGAGTA
  GCTGGCACTGGGATGGGCAGGAACAGCAATGTCTCTACTGATGGTTTCATTGGAGAAAGC
  TGGCAAAGTTGAGAAGTTTGGAGACTAACCGTGACAAAATTTAAACATCAGGTATGTGAA
  GGCAAGGGGGACCAACTGTGACAAAATGTAAACGTCAAGTGTGTGAAGTCAAAGGGGAAC
  CAGGAAAGTAAAAGAGTGACAATCAGCAGTGCTTTTGGCAAAAAGGCCTCTGGCAGCAGT
  GTAGAAGAGAAACGCAGAGATCACAGGTATAAGAATGACAATAGGAGAGGGGAGGAGGGT
  ACATATTTCAGAGGCATCACAGGGGGGTGTACTGATAGGACGGGGGCCTGCTTGTATGAA
  GGTGAAAGAGGAGGAGGGAGAGTGTCTCTGGGAGATGGGGTGAATGGTAGTGTTCTGAGC
  AGAGAGAGCAGGGAAATCANGAGGAGGAGTGGTTCAGGGAAGAAGCAGGGGTTCAGATGC
  CAAGTTCTGCATTGGCCAAGCTGAGTCAGAAGTGCTGGGAGACTCCCATGTGGGTCTGGN
  GATAGAAAGAAGCTCTGAGTTCAGCAATGATCATGGAAACCCAAGGGNAGGTTTGACAGC
  ACCCTGAGGTGGGGGGAGACATGGCACTNGAGAGGNATCTTGNATGATNCCTACATTAAA
  GACATNCTGACTGGGCACAGTGGCTCATGCCTGTAATCCCAGAAACTTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004391
• Insert Size: 4130 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004391.1, NP_004382.1
• RefSeq Size: 3951 bp
• RefSeq ORF: 1506 bp
• Locus ID: 1582
• Cytogenetics: 3p22.1
• Domains: p450
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Metabolic pathways, PPAR signaling pathway, Primary bile acid biosynthesis
• Gene Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one. The balance between these two steroids determines the relative amounts of cholic acid and chenodeoxycholic acid both of which are secreted in the bile and affect the solubility of cholesterol. This gene is unique among the cytochrome P450 genes in that it is intronless. [provided by RefSeq, Jul 2008]'