CDC14B (NM_003671) Human Tagged ORF Clone

CAT#: RG221121

CDC14B (GFP-tagged) - Human CDC14 cell division cycle 14 homolog B (S. cerevisiae) (CDC14B), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CDC14B
• Synonyms: Cdc14B1; Cdc14B2; CDC14B3; hCDC14B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221121 representing NM_003671
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCGGAAAAGCGAGCGGCGGTCGAGCTGGGCCGCCGCGCCCCCCTGCTCGCGGCGCTGCTCGTCGA
  CCTCGCCGGGTGTGAAGAAGATCCGCAGCTCCACGCAGCAAGACCCGCGCCGCCGGGACCCCCAGGACGA
  CGTGTACCTGGACATCACCGATCGCCTTTGTTTTGCCATTCTCTACAGCAGACCAAAGAGTGCATCAAAT
  GTACATTATTTCAGCATAGATAATGAACTTGAATATGAGAACTTCTACGCAGATTTTGGACCACTCAATC
  TGGCAATGGTTTACAGATATTGTTGCAAGATCAATAAGAAATTAAAGTCCATTACAATGTTAAGGAAGAA
  AATTGTTCATTTTACTGGCTCTGATCAGAGAAAACAAGCAAATGCTGCCTTCCTTGTTGGATGCTACATG
  GTTATATATTTGGGGAGAACCCCAGAAGAAGCATATAGAATATTAATCTTTGGAGAGACATCCTATATTC
  CTTTCAGAGATGCTGCCTATGGAAGTTGCAATTTCTACATTACACTTCTTGACTGTTTTCATGCAGTAAA
  GAAGGCAATGCAGTATGGCTTCCTTAATTTCAACTCATTTAACCTTGATGAATATGAACACTATGAAAAA
  GCAGAAAATGGAGATTTAAATTGGATAATACCAGACCGATTTATTGCCTTCTGTGGACCTCATTCAAGAG
  CCAGACTTGAAAGTGGTTACCACCAACATTCTCCTGAGACTTATATTCAATATTTTAAGAATCACAATGT
  TACTACCATTATTCGTCTGAATAAAAGGATGTATGATGCCAAACGCTTTACGGATGCTGGCTTCGATCAC
  CATGATCTTTTCTTTGCGGATGGCAGCACCCCTACTGATGCCATTGTCAAAGAATTCCTAGATATCTGTG
  AAAATGCTGAGGGTGCCATTGCAGTACATTGCAAAGCTGGCCTTGGTCGCACGGGCACTCTGATAGCCTG
  CTACATCATGAAGCATTACAGGATGACAGCAGCCGAGACCATTGCGTGGGTCAGGATCTGCAGACCTGGC
  TCGGTGATTGGGCCTCAGCAGCAGTTTTTGGTGATGAAGCAAACCAACCTCTGGCTGGAAGGGGACTATT
  TTCGTCAGAAGTTAAAGGGGCAGGAGAATGGACAACACAGAGCAGCCTTCTCCAAACTTCTCTCTGGCGT
  TGATGACATTTCCATAAATGGGGTCGAGAATCAAGATCAGCAAGAACCCGAACCGTACAGTGATGATGAC
  GAAATCAATGGAGTGACACAAGGTGATAGACTTCGGGCCTTGAAAAGCAGAAGACAATCCAAAACAAACG
  CTATTCCTCTCACTCTCTCCATTTCAAGGACTAAAACAGTCTTGCGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221121 representing NM_003671
  Red=Cloning site Green=Tags(s)
  
  MKRKSERRSSWAAAPPCSRRCSSTSPGVKKIRSSTQQDPRRRDPQDDVYLDITDRLCFAILYSRPKSASN
  VHYFSIDNELEYENFYADFGPLNLAMVYRYCCKINKKLKSITMLRKKIVHFTGSDQRKQANAAFLVGCYM
  VIYLGRTPEEAYRILIFGETSYIPFRDAAYGSCNFYITLLDCFHAVKKAMQYGFLNFNSFNLDEYEHYEK
  AENGDLNWIIPDRFIAFCGPHSRARLESGYHQHSPETYIQYFKNHNVTTIIRLNKRMYDAKRFTDAGFDH
  HDLFFADGSTPTDAIVKEFLDICENAEGAIAVHCKAGLGRTGTLIACYIMKHYRMTAAETIAWVRICRPG
  SVIGPQQQFLVMKQTNLWLEGDYFRQKLKGQENGQHRAAFSKLLSGVDDISINGVENQDQQEPEPYSDDD
  EINGVTQGDRLRALKSRRQSKTNAIPLTLSISRTKTVLR
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003671
• ORF Size: 1377 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003671.3, NP_003662.1
• RefSeq Size: 5472
• RefSeq ORF: 1380
• Locus ID: 8555
• Domains: Y_phosphatase, DSPc, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Cell cycle
• Gene Summary: The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]