UBE2W (NM_001001481) Human Tagged ORF Clone

CAT#: RG221130

UBE2W (GFP-tagged) - Human ubiquitin-conjugating enzyme E2W (putative) (UBE2W), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UBE2W
• Synonyms: UBC-16; UBC16
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221130 representing NM_001001481
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCAATGCAGACCACAGGAAGGAGGGTGGAAGTATGGTTTCCAAAACGACTACAGAAAGAACTGT
  TGGCTTTGCAAAATGACCCACCTCCTGGAATGACCTTAAATGAGAAGAGTGTTCAAAATTCAATTACACA
  GTGGATTGTAGACATGGAAGGTGCACCAGGTACCTTATATGAAGGGGAAAAATTTCAACTTCTATTTAAA
  TTTAGTAGTCGATATCCTTTTGACTCTCCTCAGGTCATGTTTACTGGTGAAAATATTCCTGTTCATCCTC
  ATGTTTATAGCAATGGTCATATCTGTTTATCCATTCTAACAGAAGACTGGTCCCCAGCGCTCTCAGTCCA
  ATCAGTTTGTCTTAGCATTATTAGCATGCTTTCCAGCTGCAAGGAAAAGAGACGACCACCGGATAATTCT
  TTTTATGTGCGAACATGTAACAAGAATCCAAAGAAAACAAAATGGTGGTATCATGATGATACTTGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221130 representing NM_001001481
  Red=Cloning site Green=Tags(s)
  
  MASMQTTGRRVEVWFPKRLQKELLALQNDPPPGMTLNEKSVQNSITQWIVDMEGAPGTLYEGEKFQLLFK
  FSSRYPFDSPQVMFTGENIPVHPHVYSNGHICLSILTEDWSPALSVQSVCLSIISMLSSCKEKRRPPDNS
  FYVRTCNKNPKKTKWWYHDDTC
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001481
• ORF Size: 486 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001001481.1, NP_001001481.1
• RefSeq Size: 4057
• RefSeq ORF: 489
• Locus ID: 55284
• Protein Families: Transcription Factors
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]