PTPRD (NM_130391) Human Tagged ORF Clone

CAT#: RG221383

PTPRD (GFP-tagged) - Human protein tyrosine phosphatase, receptor type, D (PTPRD), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PTPRD
• Synonyms: HPTP; HPTPD; HPTPDELTA; PTPD; RPTPDELTA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221383 representing NM_130391
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCACGTAGCCAGGCTGCTGCTGCTGCTCCTCACTTTCTTCCTCCGCACGGATGCTGAGACACCTC
  CAAGGTTTACACGAACACCCGTTGATCAGACAGGGGTCTCTGGCGGAGTTGCCTCTTTCATCTGCCAAGC
  TACGGGAGACCCAAGACCTAAAATTGTCTGGAACAAAAAAGGAAAGAAAGTCAGCAATCAGAGATTTGAG
  GTAATAGAGTTTGACGATGGGTCTGGATCAGTTCTCAGAATACAACCCTTACGGACTCCGAGGGATGAGG
  CCATTTATGAATGTGTGGCCTCAAATAATGTGGGAGAAATAAGTGTATCCACCAGACTCACAGTTTTGCG
  GGAAGATCAAATTCCCAGGGGCTTCCCTACCATTGACATGGGCCCACAGTTGAAGGTGGTTGAGCGTACT
  CGCACGGCCACCATGCTTTGTGCAGCCAGTGGTAATCCGGATCCAGAAATCACTTGGTTTAAAGATTTCT
  TACCTGTGGACACAAGCAACAACAATGGTCGTATTAAGCAGTTACGATCAGAATCTATTGGTGGTACACC
  AATAAGAGGAGCCCTTCAGATTGAGCAGAGTGAAGAGTCTGACCAAGGAAAATATGAGTGTGTTGCCACC
  AACAGCGCGGGCACTCGCTATTCCGCTCCTGCCAATTTATATGTCAGAGAGCTGCGAGAAGTTCGCCGTG
  TCCCACCAAGATTCTCTATCCCACCCACTAATCATGAAATCATGCCAGGCGGAAGCGTTAATATCACCTG
  TGTGGCCGTGGGGTCACCAATGCCTTATGTAAAGTGGATGTTGGGGGCAGAAGATCTGACACCTGAAGAT
  GATATGCCAATAGGAAGAAATGTGCTAGAACTGAATGATGTAAGACAGTCAGCAAATTACACCTGTGTTG
  CTATGTCAACACTGGGTGTCATTGAAGCAATAGCACAGATCACTGTCAAAGCCTTACCCAAACCTCCAGG
  AACTCCTGTAGTGACCGAGAGCACAGCTACAAGCATCACACTGACGTGGGACTCTGGGAACCCTGAGCCT
  GTTTCTTATTACATAATTCAGCATAAACCTAAAAACTCTGAGGAACTTTACAAAGAAATTGATGGGGTGG
  CGACCACACGCTACAGTGTCGCTGGACTAAGTCCCTACTCGGATTATGAATTCAGGGTTGTTGCTGTCAA
  TAACATTGGGCGGGGGCCTCCCAGCGAACCTGTGCTAACACAAACCTCAGAGCAAGCACCATCCAGTGCC
  CCGAGGGATGTCCAGGCACGAATGTTGAGTTCGACCACCATTTTGGTACAGTGGAAGGAACCTGAAGAGC
  CAAATGGACAGATCCAAGGATATAGAGTTTATTATACAATGGATCCCACTCAACATGTCAACAACTGGAT
  GAAACACAATGTAGCTGACAGCCAAATCACTACTATTGGCAACTTAGTGCCCCAGAAAACATATTCTGTC
  AAAGTCCTGGCTTTTACCTCAATTGGAGATGGTCCCCTTTCAAGTGACATACAAGTCATCACTCAGACAG
  GAGTACCAGGGCAGCCACTAAACTTCAAAGCAGAACCTGAGTCTGAAACAAGTATTTTGCTCTCTTGGAC
  ACCTCCACGTTCAGATACCATTGCCAACTATGAACTGGTCTACAAAGATGGGGAGCATGGAGAGGAGCAA
  CGAATTACCATTGAGCCAGGGACATCATATAGGCTGCAAGGACTGAAACCAAACAGCTTATACTATTTCC
  GTCTGGCTGCACGCTCCCCTCAAGGCCTGGGTGCTTCTACTGCAGAAATATCAGCTAGAACCATGCAGTC
  AATGTTTGCAAAAAATTTTCATGTCAAAGCAGTAATGAAGACTTCCGTGTTGCTGTCTTGGGAGATTCCA
  GAGAATTATAACTCCGCCATGCCTTTCAAAATTCTTTATGATGATGGGAAAATGGTAGAAGAAGTGGATG
  GCCGAGCCACACAGAAGTTAATTGTCAACCTGAAGCCTGAGAAATCATATTCATTTGTGCTGACAAATCG
  TGGAAACAGTGCTGGTGGGCTGCAGCACAGGGTCACGGCAAAGACTGCACCAGATGTATTACGTACCAAG
  CCTGCCTTCATTGGGAAGACCAACTTGGATGGCATGATTACTGTGCAACTGCCTGAAGTACCTGCAAATG
  AGAATATAAAAGGTTACTACATAATAATTGTGCCTTTGAAGAAATCTCGCGGGAAATTTATCAAGCCATG
  GGAGAGTCCAGATGAAATGGAATTAGATGAGCTGCTTAAGGAGATATCTAGGAAGCGCAGAAGCATCCGT
  TATGGGAGAGAAGTTGAATTAAAGCCATATATTGCCGCTCACTTTGATGTCCTTCCCACTGAGTTCACCC
  TGGGGGATGACAAGCATTATGGTGGATTTACAAACAAGCAACTCCAAAGTGGTCAAGAATATGTCTTCTT
  TGTGTTAGCAGTAATGGAACATGCAGAGTCTAAGATGTATGCAACCAGCCCTTACTCCGACCCCGTGGTG
  TCAATGGATCTGGATCCGCAGCCAATCACGGATGAAGAAGAAGGCTTGATCTGGGTTGTAGGTCCTGTCC
  TTGCAGTGGTCTTTATCATCTGCATTGTCATTGCTATTCTTCTTTATAAAAGTAAACCCGACAGGAAGAG
  GGCAGAGTCCGACTCTAGAAAAAGCAGCATACCGAACAATAAGGAGATCCCTTCACACCACCCAACAGAC
  CCTGTAGAACTGAGGCGCCTTAACTTTCAAACACCGGGTATGGCTAGCCATCCTCCAATACCCATCTTGG
  AACTTGCAGACCACATTGAAAGATTGAAAGCAAATGACAACTTGAAGTTTTCCCAGGAATATGAGTCAAT
  TGACCCTGGCCAGCAGTTCACTTGGGAACATTCAAACTTGGAAGTAAACAAACCAAAGAATAGATACGCG
  AATGTAATCGCATATGATCATTCCCGGGTTCTCCTATCAGCTATAGAAGGGATCCCAGGAAGTGACTATG
  TGAATGCCAACTACATAGATGGGTATAGGAAGCAAAATGCCTATATTGCAACACAGGGATCTCTCCCCGA
  AACATTTGGGGACTTTTGGAGAATGATATGGGAACAACGGAGTGCCACAGTTGTCATGATGACAAAACTA
  GAAGAAAGATCAAGGGTGAAGTGTGACCAGTATTGGCCTAGCAGAGGCACAGAAACCCACGGACTCGTTC
  AAGTAACGCTGCTTGATACTGTGGAGCTGGCCACATATTGTGTTCGAACATTTGCACTTTACAAGAATGG
  TTCAAGTGAGAAGAGAGAAGTGAGACAATTCCAGTTCACCGCCTGGCCTGATCATGGTGTTCCAGAACAC
  CCTACACCTTTTCTAGCTTTCTTACGTAGAGTCAAAACCTGTAACCCTCCCGATGCTGGTCCGATGGTTG
  TGCACTGCAGTGCGGGAGTTGGCCGGACTGGTTGCTTCATCGTCATAGATGCCATGTTAGAAAGAATAAA
  GCATGAAAAAACTGTAGATATTTATGGCCATGTAACTTTAATGAGAGCCCAGAGGAACTATATGGTTCAA
  ACAGAAGACCAATACATCTTTATCCATGATGCACTGTTAGAAGCAGTGACTTGTGGAAATACCGAAGTGC
  CAGCTAGAAACTTGTATGCCTACATTCAGAAGCTGACACAAATAGAAACGGGAGAGAATGTCACAGGAAT
  GGAGCTCGAATTTAAGCGTCTAGCCAGCTCAAAAGCTCACACCTCAAGGTTTATCAGTGCCAATCTTCCA
  TGTAATAAATTCAAAAATCGCCTTGTTAATATTATGCCATATGAATCCACAAGGGTATGCCTGCAGCCTA
  TCCGTGGAGTAGAAGGATCTGATTACATCAATGCCAGTTTTATTGATGGATACAGACAACAGAAAGCCTA
  CATCGCTACCCAGGGGCCCTTGGCAGAGACCACTGAAGACTTCTGGCGGATGCTCTGGGAACACAATTCC
  ACCATAGTTGTGATGCTCACCAAGCTGCGTGAAATGGGCAGAGAGAAATGTCACCAATACTGGCCAGCAG
  AACGGTCTGCAAGATACCAGTACTTTGTTGTAGATCCCATGGCTGAGTACAACATGCCACAGTATATCCT
  AAGGGAATTCAAGGTCACAGATGCCAGGGACGGCCAGTCCCGAACAGTAAGGCAGTTCCAGTTCACTGAC
  TGGCCAGAGCAAGGAGTGCCAAAGTCCGGAGAAGGATTTATTGACTTCATCGGCCAAGTCCATAAAACAA
  AAGAACAGTTTGGCCAAGATGGACCCATTTCAGTCCATTGCAGCGCGGGCGTTGGAAGAACTGGAGTCTT
  CATAACGCTAAGCATTGTTTTGGAAAGAATGAGATATGAAGGAGTTGTAGATATCTTCCAGACTGTCAAA
  ATGTTAAGAACACAACGACCAGCTATGGTACAGACAGAGGATCAATATCAGTTTTCCTATCGTGCCGCAC
  TAGAGTACCTGGGCAGCTTTGACCACTATGCAACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221383 representing NM_130391
  Red=Cloning site Green=Tags(s)
  
  MVHVARLLLLLLTFFLRTDAETPPRFTRTPVDQTGVSGGVASFICQATGDPRPKIVWNKKGKKVSNQRFE
  VIEFDDGSGSVLRIQPLRTPRDEAIYECVASNNVGEISVSTRLTVLREDQIPRGFPTIDMGPQLKVVERT
  RTATMLCAASGNPDPEITWFKDFLPVDTSNNNGRIKQLRSESIGGTPIRGALQIEQSEESDQGKYECVAT
  NSAGTRYSAPANLYVRELREVRRVPPRFSIPPTNHEIMPGGSVNITCVAVGSPMPYVKWMLGAEDLTPED
  DMPIGRNVLELNDVRQSANYTCVAMSTLGVIEAIAQITVKALPKPPGTPVVTESTATSITLTWDSGNPEP
  VSYYIIQHKPKNSEELYKEIDGVATTRYSVAGLSPYSDYEFRVVAVNNIGRGPPSEPVLTQTSEQAPSSA
  PRDVQARMLSSTTILVQWKEPEEPNGQIQGYRVYYTMDPTQHVNNWMKHNVADSQITTIGNLVPQKTYSV
  KVLAFTSIGDGPLSSDIQVITQTGVPGQPLNFKAEPESETSILLSWTPPRSDTIANYELVYKDGEHGEEQ
  RITIEPGTSYRLQGLKPNSLYYFRLAARSPQGLGASTAEISARTMQSMFAKNFHVKAVMKTSVLLSWEIP
  ENYNSAMPFKILYDDGKMVEEVDGRATQKLIVNLKPEKSYSFVLTNRGNSAGGLQHRVTAKTAPDVLRTK
  PAFIGKTNLDGMITVQLPEVPANENIKGYYIIIVPLKKSRGKFIKPWESPDEMELDELLKEISRKRRSIR
  YGREVELKPYIAAHFDVLPTEFTLGDDKHYGGFTNKQLQSGQEYVFFVLAVMEHAESKMYATSPYSDPVV
  SMDLDPQPITDEEEGLIWVVGPVLAVVFIICIVIAILLYKSKPDRKRAESDSRKSSIPNNKEIPSHHPTD
  PVELRRLNFQTPGMASHPPIPILELADHIERLKANDNLKFSQEYESIDPGQQFTWEHSNLEVNKPKNRYA
  NVIAYDHSRVLLSAIEGIPGSDYVNANYIDGYRKQNAYIATQGSLPETFGDFWRMIWEQRSATVVMMTKL
  EERSRVKCDQYWPSRGTETHGLVQVTLLDTVELATYCVRTFALYKNGSSEKREVRQFQFTAWPDHGVPEH
  PTPFLAFLRRVKTCNPPDAGPMVVHCSAGVGRTGCFIVIDAMLERIKHEKTVDIYGHVTLMRAQRNYMVQ
  TEDQYIFIHDALLEAVTCGNTEVPARNLYAYIQKLTQIETGENVTGMELEFKRLASSKAHTSRFISANLP
  CNKFKNRLVNIMPYESTRVCLQPIRGVEGSDYINASFIDGYRQQKAYIATQGPLAETTEDFWRMLWEHNS
  TIVVMLTKLREMGREKCHQYWPAERSARYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTVRQFQFTD
  WPEQGVPKSGEGFIDFIGQVHKTKEQFGQDGPISVHCSAGVGRTGVFITLSIVLERMRYEGVVDIFQTVK
  MLRTQRPAMVQTEDQYQFSYRAALEYLGSFDHYAT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130391
• ORF Size: 4515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130391.3, NP_569075.2
• RefSeq Size: 8266 bp
• RefSeq ORF: 4518 bp
• Locus ID: 5789
• Cytogenetics: 9p24.1-p23
• Domains: Y_phosphatase, ig, PTPc_motif, IGc2, IG, FN3
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular region, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this protein is composed of three Ig-like and eight fibronectin type III-like domains. Studies of the similar genes in chicken and fly suggest the role of this PTP is in promoting neurite growth, and regulating neurons axon guidance. Multiple alternatively spliced transcript variants of this gene have been reported. A related pseudogene has been identified on chromosome 5. [provided by RefSeq, Jan 2010]'