CHRNE (NM_000080) Human Tagged ORF Clone
CAT#: RG221423
- TrueORF®
CHRNE (GFP-tagged) - Human cholinergic receptor, nicotinic, epsilon (CHRNE)
"NM_000080" in other vectors (6)
Product Images
![](https://cdn.origene.com/img/defaults-img-expression-plasmids.jpg)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | CHRNE |
Synonyms | ACHRE; CMS1D; CMS1E; CMS2A; CMS4A; CMS4B; CMS4C; FCCMS; SCCMS |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG221423 representing NM_000080
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGGGCTCCGCTTGGGGTCCTGCTCCTCTTGGGGCTTCTCGGCAGGGGTGTGGGGAAGAACGAGG AACTGCGTCTTTATCACCATCTCTTCAACAACTATGACCCAGGAAGCCGGCCAGTGCGGGAGCCTGAGGA TACTGTCACCATCAGCCTCAAGGTCACCCTGACGAATCTCATCTCACTGAATGAAAAAGAGGAGACTCTC ACCACTAGCGTCTGGATTGGAATCGATTGGCAGGATTACCGACTCAACTACAGCAAGGACGACTTTGGGG GTATAGAAACCCTGCGAGTCCCTTCAGAACTCGTGTGGCTGCCAGAGATTGTGCTGGAAAACAATATTGA TGGCCAGTTCGGAGTGGCCTACGACGCCAACGTGCTCGTCTACGAGGGCGGCTCCGTGACGTGGCTGCCT CCGGCCATCTACCGCAGCGTCTGCGCAGTGGAGGTCACCTACTTCCCCTTCGATTGGCAGAACTGTTCGC TTATTTTCCGCTCTCAGACGTACAATGCCGAAGAGGTGGAGTTCACTTTTGCCGTAGACAACGACGGCAA GACCATCAACAAGATCGACATCGACACAGAGGCCTATACTGAGAACGGCGAGTGGGCCATCGACTTCTGC CCGGGGGTGATCCGCCGCCACCACGGTGGCGCCACCGACGGCCCAGGGGAGACTGACGTCATCTACTCGC TCATCATCCGCCGGAAGCCGCTCTTCTACGTCATTAACATCATCGTGCCCTGTGTGCTCATCTCGGGCCT GGTGCTGCTCGCCTACTTCCTGCCGGCGCAGGCCGGCGGCCAGAAATGCACGGTCTCCATCAACGTCCTG CTCGCCCAGACCGTCTTCTTGTTCCTCATTGCCCAGAAAATCCCAGAGACTTCTCTGAGCGTGCCGCTCC TGGGCAGGTTCCTTATTTTCGTCATGGTGGTCGCCACGCTCATTGTCATGAATTGCGTCATCGTGCTCAA CGTGTCCCAGCGGACGCCCACCACCCACGCCATGTCCCCGCGGCTGCGCCACGTTCTCCTGGAGCTGCTG CCGCGCCTCCTGGGCTCCCCGCCGCCGCCCGAGGCCCCCCGGGCCGCCTCGCCCCCAAGGCGGGCGTCGT CGGTGGGCTTATTGCTCCGCGCGGAGGAGCTGATACTGAAAAAGCCACGGAGCGAGCTCGTGTTTGAGGG GCAGAGGCACCGGCAGGGGACCTGGACGGCTGCCTTCTGCCAGAGCCTGGGCGCCGCCGCCCCCGAGGTC CGCTGCTGTGTGGATGCCGTGAACTTCGTGGCCGAGAGCACGAGAGATCAGGAGGCCACCGGCGAGGAAG TGTCCGACTGGGTGCGCATGGGGAATGCCCTTGACAACATCTGCTTCTGGGCCGCTCTGGTGCTCTTCAG CGTGGGCTCCAGCCTCATCTTCCTCGGGGCCTACTTCAACCGAGTGCCTGATCTCCCCTACGCGCCGTGT ATCCAGCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG221423 representing NM_000080
Red=Cloning site Green=Tags(s) MARAPLGVLLLLGLLGRGVGKNEELRLYHHLFNNYDPGSRPVREPEDTVTISLKVTLTNLISLNEKEETL TTSVWIGIDWQDYRLNYSKDDFGGIETLRVPSELVWLPEIVLENNIDGQFGVAYDANVLVYEGGSVTWLP PAIYRSVCAVEVTYFPFDWQNCSLIFRSQTYNAEEVEFTFAVDNDGKTINKIDIDTEAYTENGEWAIDFC PGVIRRHHGGATDGPGETDVIYSLIIRRKPLFYVINIIVPCVLISGLVLLAYFLPAQAGGQKCTVSINVL LAQTVFLFLIAQKIPETSLSVPLLGRFLIFVMVVATLIVMNCVIVLNVSQRTPTTHAMSPRLRHVLLELL PRLLGSPPPPEAPRAASPPRRASSVGLLLRAEELILKKPRSELVFEGQRHRQGTWTAAFCQSLGAAAPEV RCCVDAVNFVAESTRDQEATGEEVSDWVRMGNALDNICFWAALVLFSVGSSLIFLGAYFNRVPDLPYAPC IQP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000080 |
ORF Size | 1479 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000080.2, NP_000071.1 |
RefSeq Size | 2463 bp |
RefSeq ORF | 1482 bp |
Locus ID | 1145 |
Cytogenetics | 17p13.2 |
Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
Gene Summary | 'Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome. [provided by RefSeq, Sep 2009]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC313709 | CHRNE (untagged)-Human cholinergic receptor, nicotinic, epsilon (CHRNE) |
USD 830.00 |
|
RC221423 | CHRNE (Myc-DDK-tagged)-Human cholinergic receptor, nicotinic, epsilon (CHRNE) |
USD 420.00 |
|
RC221423L1 | Lenti ORF clone of Human cholinergic receptor, nicotinic, epsilon (CHRNE), Myc-DDK-tagged |
USD 768.00 |
|
RC221423L2 | Lenti ORF clone of Human cholinergic receptor, nicotinic, epsilon (CHRNE), mGFP tagged |
USD 620.00 |
|
RC221423L3 | Lenti ORF clone of Human cholinergic receptor, nicotinic, epsilon (CHRNE), Myc-DDK-tagged |
USD 620.00 |
|
RC221423L4 | Lenti ORF clone of Human cholinergic receptor, nicotinic, epsilon (CHRNE), mGFP tagged |
USD 620.00 |
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