CHRNE (NM_000080) Human Untagged Clone

CAT#: SC313709

CHRNE (untagged)-Human cholinergic receptor, nicotinic, epsilon (CHRNE)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHRNE
• Synonyms: ACHRE; CMS1D; CMS1E; CMS2A; CMS4A; CMS4B; CMS4C; FCCMS; SCCMS
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000080 edited
  GGATGGGAGCTACAGTTTTTGTCCAGCCCCTGGACCTGGTGAAGAACCGGATGCAGTTGA
  GCGGGGAAGGGGCCAAGACTCGAGAGTACAAAACCAGCTTCCATGCCCTCACCAGTATCC
  TGAAGGCAGAAGGCCTGAGGGGCATTTACACTGGGCTGTCGGCTGGCCTGCTGCGTCAGG
  CCACCTACACCACTACCCGCCTTGGCATCTATACCGTGCTGTTTGAGCGCCTGACTGGGG
  CTGATGGTACTCCCCCTGGCTTTCTGCTGAAGGCTGTGATTGGCATGACCGCAGGTGCCA
  CTGGTGCCTTTGTGGGAACACCAGCCGAAGTGGCTCTTATCCGCATGACTGCCGATGGCC
  GGCTTCCAGCTGACCAGCGCCGTGGCTACAAAAATGTGTTTAACGCCCTGATTCGAATCA
  CCCGGGAAGAGGGTGTCCTCACACTGTGGCGGGGCTGCATCCCTACCATGGCTCGGGCCG
  TCGTCGTCAATGCTGCCCAGCTCGCCTCCTACTCCCAATCCAAGCAGTTCTTACTGGACT
  CAGGCTACTTCTCTGACAACATCTTGTGCCACTTCTGTGCCAGCATGATCAGCGGTCTTG
  TCACCACTGCTGCCTCCATGCCTGTGGACATTGCCAAGACCCGAATCCAGAACATGCGGA
  TGATTGATGGGAAGCCGGAATACAAGAACGGGCTGGACGTGCTGTTCAAAGTTGTCCGCT
  ACGAGGGCTTCTTCAGCCTGTGGAAGGGCTTCACGCCGTACTATGCCCGCCTGGGCCCCC
  ACACCGTCCTCACCTTCATCTTCTTGGAGCAGATGAACAAGGCCTACAAGCGTCTCTTCC
  TCAGTGGCTGAAGCGGCCGGGGGCTCCCACTCGCCTGCTGCGCCTATAGCCACTGCGCCC
  TGGGGGCCTGGGCTCTGCTGCCCTGGACCCCTCTATTTATTTCCCTTCCACAGTGTGGTT
  TCTTCCTCTGCGGTCCTGACCACGACCCTGACTCAGAGCTGAGGGCCTCCTTCCTGCCAC
  TAGAGGCCCCACACGGCCATTAGGCCGTACCCACAGGAAGAGGCTGGAGCGGAAAGTGGG
  AAGCGAACCTCGAAGGAAGAGCAGCCAGGCCCGGGGCACTGTCACTTGCCGTCCCCTCTG
  CAGCTCCAGCCTTGACGCGGAAGCTCTGGAATAAGCAGCAGCACAGATTTGAGACTTAGT
  CCATGTACCAGCCACATGCAAAGAATGGCAAGGGCTCCGCTTGGGGTCCTGCTCCTCTTG
  GGGCTTCTCGGCAGGGGTGTGGGGAAGAACGAGGAACTGCGTCTTTATCACCATCTCTTC
  AACAACTATGACCCAGGAAGCCGGCCAGTGCGGGAGCCTGAGGATACTGTCACCATCAGC
  CTCAAGGTCACCCTGACGAATCTCATCTCACTGAATGAAAAAGAGGAGACTCTCACCACT
  AGCGTCTGGATTGGAATCGATTGGCAGGATTACCGACTCAACTACAGCAAGGACGACTTT
  GGGGGTATAGAAACCCTGCGAGTCCCTTCAGAACTCGTGTGGCTGCCAGAGATTGTGCTG
  GAAAACAATATTGATGGCCAGTTCGGAGTGGCCTACGACGCCAACGTGCTCGTCTACGAG
  GGCGGCTCCGTGACGTGGCTGCCTCCGGCCATCTACCGCAGCGTCTGCGCAGTGGAGGTC
  ACCTACTTCCCCTTCGATTGGCAGAACTGTTCGCTTATTTTCCGCTCTCAGACGTACAAT
  GCCGAAGAGGTGGAGTTCACTTTTGCCGTAGACAACGACGGCAAGACCATCAACAAGATC
  GACATCGACACAGAGGCCTATACTGAGAACGGCGAGTGGGCCATCGACTTCTGCCCGGGG
  GTGATCCGCCGCCACCACGGTGGCGCCACCGACGGCCCAGGGGAGACTGACGTCATCTAC
  TCGCTCATCATCCGCCGGAAGCCGCTCTTCTACGTCATTAACATCATCGTGCCCTGTGTG
  CTCATCTCGGGCCTGGTGCTGCTCGCCTACTTCCTGCCGGCGCAGGCCGGCGGCCAGAAA
  TGCACGGTCTCCATCAACGTCCTGCTCGCCCAGACCGTCTTCTTGTTCCTCATTGCCCAG
  AAAATCCCAGAGACTTCTCTGAGCGTGCCGCTCCTGGGCAGGTTCCTTATTTTCGTCATG
  GTGGTCGCCACGCTCATTGTCATGAATTGCGTCATCGTGCTCAACGTGTCCCAGCGGACG
  CCCACCACCCACGCCATGTCCCCGCGGCTGCGCCACGTTCTCCTGGAGCTGCTGCCGCGC
  CTCCTGGGCTCCCCGCCGCCGCCCGAGGCCCCCCGGGCCGCCTCGCCCCCAAGGCGGGCG
  TCGTCGGTGGGCTTATTGCTCCGCGCGGAGGAGCTGATACTGAAAAAGCCACGGAGCGAG
  CTCGTGTTTGAGGGGCAGAGGCACCGGCAGGGGACCTGGACGGCTGCCTTCTGCCAGAGC
  CTGGGCGCCGCCGCCCCCGAGGTCCGCTGCTGTGTGGATGCCGTGAACTTCGTGGCCGAG
  AGCACGAGAGATCAGGAGGCCACCGGCGAGGAAGTGTCCGACTGGGTGCGCATGGGGAAT
  GCCCTTGACAACATCTGCTTCTGGGCCGCTCTGGTGCTCTTCAGCGTGGGCTCCAGCCTC
  ATCTTCCTCGGGGCCTACTTCAACCGAGTGCCTGATCTCCCCTACGCGCCGTGTATCCAG
  CCTTAGCTCGCACCGACTTCAATTTCCCACCCATCTCCAGTAGGAAATTGATTTTGAAAA
  AGTAGGCTGCCGCCACCACGGCATTATGATCCCTTCCCCCTGCTGATCAATCTGCAGTTT
  GTGAACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000080
• Insert Size: 2800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone has been fully sequenced and found to be a perfect match to the protein associated with this reference, NM_000080.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000080.2, NP_000071.1
• RefSeq Size: 2463 bp
• RefSeq ORF: 1482 bp
• Locus ID: 1145
• Cytogenetics: 17p13.2
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Gene Summary: 'Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome. [provided by RefSeq, Sep 2009]'