ATP2B2 (NM_001001331) Human Tagged ORF Clone

CAT#: RG221710

ATP2B2 (GFP-tagged) - Human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ATP2B2
• Synonyms: PMCA2; PMCA2a; PMCA2i
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221710 representing NM_001001331
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGACATGACCAACAGCGACTTTTACTCCAAAAACCAAAGAAATGAGTCGAGCCATGGGGGCGAGT
  TCGGGTGCACAATGGAGGAGCTCCGCTCCCTCATGGAGCTGCGGGGCACTGAGGCTGTGGTCAAGATCAA
  GGAGACTTATGGGGACACCGAAGCCATCTGCCGGCGCCTCAAAACCTCACCTGTTGAAGGTTTGCCGGGC
  ACCGCTCCAGACCTGGAAAAGAGAAAGCAAATTTTTGGGCAAAACTTTATACCTCCAAAGAAGCCAAAAA
  CCTTCCTGCAGCTCGTGTGGGAGGCGCTGCAGGACGTGACGCTCATCATCCTGGAGATTGCCGCCATCAT
  CTCCCTGGGGCTGTCCTTCTACCACCCGCCCGGCGAGGGCAACGAAGGATGTGCGACGGCCCAGGGTGGG
  GCAGAGGATGAAGGAGAGGCAGAGGCAGGTTGGATCGAGGGGGCCGCCATTCTCCTCTCAGTTATCTGTG
  TGGTCCTGGTCACGGCCTTCAATGACTGGAGCAAAGAGAAACAGTTCCGGGGCCTGCAGAGCCGCATCGA
  GCAGGAACAGAAATTTACCGTGGTCCGGGCTGGCCAGGTGGTCCAGATCCCTGTGGCTGAGATCGTGGTT
  GGGGACATAGCCCAGGTCAAATATGGTGACCTCCTCCCTGCCGACGGCCTCTTCATCCAGGGCAATGACC
  TCAAGATTGATGAAAGCTCCCTAACTGGAGAGTCTGACCAGGTGCGCAAGTCCGTGGACAAGGACCCCAT
  GCTGCTGTCAGGAACCCACGTGATGGAGGGCTCAGGACGGATGTTGGTGACTGCTGTGGGTGTGAACTCT
  CAGACTGGCATCATCTTTACCCTCCTGGGGGCTGGTGGTGAAGAGGAAGAGAAGAAAGACAAAAAAGGTG
  TGAAGAAGGGGGATGGCCTTCAGCTACCAGCAGCAGACGGTGCGGCAGCTTCAAATGCTGCAGATAGTGC
  GAATGCCAGCCTAGTCAATGGTAAAATGCAGGATGGCAATGTGGACGCCAGCCAGAGCAAAGCCAAACAA
  CAGGACGGGGCAGCCGCCATGGAGATGCAGCCCCTCAAGAGTGCCGAGGGCGGCGACGCTGACGACAGGA
  AGAAGGCCAGCATGCACAAGAAGGAGAAGTCCGTGCTGCAGGGCAAGCTCACCAAGCTGGCTGTGCAGAT
  CGGGAAGGCGGGCTTGGTGATGTCAGCCATCACGGTGATCATCCTGGTGCTCTACTTCACTGTGGACACC
  TTCGTGGTCAACAAGAAGCCGTGGCTGCCTGAGTGCACGCCCGTCTACGTGCAGTACTTTGTCAAGTTCT
  TCATCATTGGCGTGACGGTGCTGGTGGTCGCCGTGCCCGAGGGGCTCCCTCTGGCCGTCACCATCTCGTT
  GGCCTATTCGGTGAAGAAAATGATGAAGGACAACAACCTGGTACGCCACCTGGATGCCTGTGAGACCATG
  GGCAATGCCACAGCCATCTGCTCAGACAAGACAGGCACGCTGACCACCAATCGCATGACAGTGGTACAGG
  CCTATGTCGGCGACGTCCACTATAAAGAGATCCCCGACCCCAGCTCCATCAACACCAAGACCATGGAGCT
  GCTGATCAATGCCATCGCCATCAACAGCGCCTACACCACCAAGATTCTGCCCCCAGAGAAGGAGGGCGCC
  CTGCCTCGGCAGGTGGGCAACAAGACGGAGTGCGGCCTGCTGGGCTTCGTGCTGGACCTGAAGCAGGACT
  ACGAGCCCGTGCGCAGCCAGATGCCAGAGGAGAAGTTGTACAAAGTGTACACCTTCAACTCCGTGCGCAA
  GTCCATGAGCACTGTCATCAAGCTGCCCGACGAGAGCTTCCGCATGTACAGCAAGGGGGCTTCTGAGATC
  GTGCTCAAGAAGTGCTGCAAAATCCTCAATGGGGCGGGAGAGCCTCGTGTCTTCCGGCCCCGCGACCGGG
  ACGAGATGGTAAAGAAGGTGATTGAGCCCATGGCTTGCGATGGGCTCCGCACTATCTGCGTGGCCTACCG
  CGACTTCCCCAGCAGCCCGGAGCCGGACTGGGACAATGAGAATGACATCCTCAACGAACTCACCTGCATC
  TGCGTGGTGGGCATCGAGGACCCGGTGCGGCCAGAGGTCCCAGAAGCCATCCGCAAGTGCCAGCGGGCAG
  GCATCACGGTCCGCATGGTCACTGGCGACAATATCAACACGGCTCGGGCCATCGCCATCAAGTGTGGCAT
  CATCCATCCTGGGGAGGACTTTCTGTGCCTCGAGGGCAAGGAGTTCAACAGGAGGATCCGCAACGAGAAG
  GGGGAGATTGAGCAGGAGCGAATTGACAAGATCTGGCCAAAGCTGCGGGTGCTGGCTCGCTCCTCCCCAA
  CGGACAAGCATACCCTGGTTAAAGGCATCATCGACAGCACACACACTGAGCAGCGGCAGGTGGTGGCCGT
  GACGGGGGACGGGACCAACGACGGGCCTGCACTCAAGAAGGCCGACGTGGGCTTCGCCATGGGCATCGCA
  GGCACTGACGTGGCCAAGGAGGCCTCAGACATCATCCTGACAGACGACAATTTCAGCAGCATCGTCAAGG
  CAGTGATGTGGGGCCGCAACGTCTATGACAGCATCTCCAAATTCTTGCAGTTCCAGCTCACCGTCAACGT
  GGTGGCCGTGATTGTGGCCTTCACAGGCGCCTGCATCACGCAGGACTCCCCTCTGAAGGCCGTGCAGATG
  CTCTGGGTGAACCTCATCATGGACACGTTTGCCTCGCTGGCACTGGCCACTGAGCCGCCCACGGAGACCC
  TGCTGCTGAGGAAGCCGTACGGCCGCAACAAGCCGCTCATCTCCAGGACCATGATGAAGAACATCCTGGG
  CCATGCTGTCTACCAGCTTGCCCTCATCTTCACCCTGCTCTTTGTTGGCGAGAAGATGTTCCAGATCGAC
  AGCGGGAGGAACGCGCCCCTGCATTCGCCACCCTCAGAACATTACACCATCATCTTCAACACCTTCGTCA
  TGATGCAGCTCTTCAACGAGATCAACGCCCGCAAGATCCACGGCGAGCGCAATGTCTTTGACGGCATCTT
  CCGGAACCCCATCTTCTGCACCATCGTGCTGGGCACCTTTGCCATCCAGATAGTGATCGTGCAGTTTGGA
  GGGAAGCCATTCAGCTGCTCTCCACTGCAGCTGGACCAGTGGATGTGGTGCATATTCATTGGGTTAGGAG
  AGCTCGTTTGGGGCCAGGTCATCGCCACCATCCCGACCAGCAGACTCAAGTTCCTCAAGGAGGCAGGCAG
  GCTCACACAGAAGGAGGAGATCCCGGAGGAGGAGCTCAACGAGGACGTGGAGGAGATCGACCACGCGGAG
  CGGGAGCTGCGGCGGGGCCAGATCCTGTGGTTCCGAGGCCTGAATCGGATCCAGACACAGATCCGCGTCG
  TGAAGGCGTTCCGTAGCTCTCTCTATGAAGGTTTAGAAAAGCCTGAATCTCGAACCTCCATCCATAACTT
  CATGGCTCATCCTGAATTCCGGATCGAAGATTCCCAGCCCCACATCCCCCTCATTGATGACACCGACCTG
  GAAGAAGATGCCGCGCTCAAGCAGAACTCGAGCCCGCCGTCATCCCTCAACAAGAACAACAGCGCCATCG
  ACAGTGGGATCAACCTGACGACCGACACAAGCAAATCAGCTACCTCTTCAAGTCCAGGGAGCCCCATCCA
  CAGCCTGGAGACGTCGCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221710 representing NM_001001331
  Red=Cloning site Green=Tags(s)
  
  MGDMTNSDFYSKNQRNESSHGGEFGCTMEELRSLMELRGTEAVVKIKETYGDTEAICRRLKTSPVEGLPG
  TAPDLEKRKQIFGQNFIPPKKPKTFLQLVWEALQDVTLIILEIAAIISLGLSFYHPPGEGNEGCATAQGG
  AEDEGEAEAGWIEGAAILLSVICVVLVTAFNDWSKEKQFRGLQSRIEQEQKFTVVRAGQVVQIPVAEIVV
  GDIAQVKYGDLLPADGLFIQGNDLKIDESSLTGESDQVRKSVDKDPMLLSGTHVMEGSGRMLVTAVGVNS
  QTGIIFTLLGAGGEEEEKKDKKGVKKGDGLQLPAADGAAASNAADSANASLVNGKMQDGNVDASQSKAKQ
  QDGAAAMEMQPLKSAEGGDADDRKKASMHKKEKSVLQGKLTKLAVQIGKAGLVMSAITVIILVLYFTVDT
  FVVNKKPWLPECTPVYVQYFVKFFIIGVTVLVVAVPEGLPLAVTISLAYSVKKMMKDNNLVRHLDACETM
  GNATAICSDKTGTLTTNRMTVVQAYVGDVHYKEIPDPSSINTKTMELLINAIAINSAYTTKILPPEKEGA
  LPRQVGNKTECGLLGFVLDLKQDYEPVRSQMPEEKLYKVYTFNSVRKSMSTVIKLPDESFRMYSKGASEI
  VLKKCCKILNGAGEPRVFRPRDRDEMVKKVIEPMACDGLRTICVAYRDFPSSPEPDWDNENDILNELTCI
  CVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKCGIIHPGEDFLCLEGKEFNRRIRNEK
  GEIEQERIDKIWPKLRVLARSSPTDKHTLVKGIIDSTHTEQRQVVAVTGDGTNDGPALKKADVGFAMGIA
  GTDVAKEASDIILTDDNFSSIVKAVMWGRNVYDSISKFLQFQLTVNVVAVIVAFTGACITQDSPLKAVQM
  LWVNLIMDTFASLALATEPPTETLLLRKPYGRNKPLISRTMMKNILGHAVYQLALIFTLLFVGEKMFQID
  SGRNAPLHSPPSEHYTIIFNTFVMMQLFNEINARKIHGERNVFDGIFRNPIFCTIVLGTFAIQIVIVQFG
  GKPFSCSPLQLDQWMWCIFIGLGELVWGQVIATIPTSRLKFLKEAGRLTQKEEIPEEELNEDVEEIDHAE
  RELRRGQILWFRGLNRIQTQIRVVKAFRSSLYEGLEKPESRTSIHNFMAHPEFRIEDSQPHIPLIDDTDL
  EEDAALKQNSSPPSSLNKNNSAIDSGINLTTDTSKSATSSSPGSPIHSLETSL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001331
• ORF Size: 3729 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001331.1, NP_001001331.1
• RefSeq Size: 6821 bp
• RefSeq ORF: 3732 bp
• Locus ID: 491
• Cytogenetics: 3p25.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'