ATP2B2 (NM_001001331) Human Untagged Clone

CAT#: SC300165

ATP2B2 (untagged)-Human ATPase, Ca++ transporting, plasma membrane 2 (ATP2B2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP2B2
• Synonyms: PMCA2; PMCA2a; PMCA2i
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001001331, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGTGACATGACCAACAGCGACTTTTACTCCAAAAACCAAAGAAATGAGTCGAGCCATGGGGGCGAGT
  TCGGGTGCACAATGGAGGAGCTCCGCTCCCTCATGGAGCTGCGGGGCACTGAGGCTGTGGTCAAGATCAA
  GGAGACTTATGGGGACACCGAAGCCATCTGCCGGCGCCTCAAAACCTCACCTGTTGAAGGTTTGCCGGGC
  ACCGCTCCAGACCTGGAAAAGAGAAAGCAAATTTTTGGGCAAAACTTTATACCTCCAAAGAAGCCAAAAA
  CCTTCCTGCAGCTCGTGTGGGAGGCGCTGCAGGACGTGACGCTCATCATCCTGGAGATTGCCGCCATCAT
  CTCCCTGGGGCTGTCCTTCTACCACCCGCCCGGCGAGGGCAACGAAGGATGTGCGACGGCCCAGGGTGGG
  GCAGAGGATGAAGGAGAGGCAGAGGCAGGTTGGATCGAGGGGGCCGCCATTCTCCTCTCAGTTATCTGTG
  TGGTCCTGGTCACGGCCTTCAATGACTGGAGCAAAGAGAAACAGTTCCGGGGCCTGCAGAGCCGCATCGA
  GCAGGAACAGAAATTTACCGTGGTCCGGGCTGGCCAGGTGGTCCAGATCCCTGTGGCTGAGATCGTGGTT
  GGGGACATAGCCCAGGTCAAATATGGTGACCTCCTCCCTGCCGACGGCCTCTTCATCCAGGGCAATGACC
  TCAAGATTGATGAAAGCTCCCTAACTGGAGAGTCTGACCAGGTGCGCAAGTCCGTGGACAAGGACCCCAT
  GCTGCTGTCAGGAACCCACGTGATGGAGGGCTCAGGACGGATGTTGGTGACTGCTGTGGGTGTGAACTCT
  CAGACTGGCATCATCTTTACCCTCCTGGGGGCTGGTGGTGAAGAGGAAGAGAAGAAAGACAAAAAAGGTG
  TGAAGAAGGGGGATGGCCTTCAGCTACCAGCAGCAGACGGTGCGGCAGCTTCAAATGCTGCAGATAGTGC
  GAATGCCAGCCTAGTCAATGGTAAAATGCAGGATGGCAATGTGGACGCCAGCCAGAGCAAAGCCAAACAA
  CAGGACGGGGCAGCCGCCATGGAGATGCAGCCCCTCAAGAGTGCCGAGGGCGGCGACGCTGACGACAGGA
  AGAAGGCCAGCATGCACAAGAAGGAGAAGTCCGTGCTGCAGGGCAAGCTCACCAAGCTGGCTGTGCAGAT
  CGGGAAGGCGGGCTTGGTGATGTCAGCCATCACGGTGATCATCCTGGTGCTCTACTTCACTGTGGACACC
  TTCGTGGTCAACAAGAAGCCGTGGCTGCCTGAGTGCACGCCCGTCTACGTGCAGTACTTTGTCAAGTTCT
  TCATCATTGGCGTGACGGTGCTGGTGGTCGCCGTGCCCGAGGGGCTCCCTCTGGCCGTCACCATCTCGTT
  GGCCTATTCGGTGAAGAAAATGATGAAGGACAACAACCTGGTACGCCACCTGGATGCCTGTGAGACCATG
  GGCAATGCCACAGCCATCTGCTCAGACAAGACAGGCACGCTGACCACCAATCGCATGACAGTGGTACAGG
  CCTATGTCGGCGACGTCCACTATAAAGAGATCCCCGACCCCAGCTCCATCAACACCAAGACCATGGAGCT
  GCTGATCAATGCCATCGCCATCAACAGCGCCTACACCACCAAGATTCTGCCCCCAGAGAAGGAGGGCGCC
  CTGCCTCGGCAGGTGGGCAACAAGACGGAGTGCGGCCTGCTGGGCTTCGTGCTGGACCTGAAGCAGGACT
  ACGAGCCCGTGCGCAGCCAGATGCCAGAGGAGAAGTTGTACAAAGTGTACACCTTCAACTCCGTGCGCAA
  GTCCATGAGCACTGTCATCAAGCTGCCCGACGAGAGCTTCCGCATGTACAGCAAGGGGGCTTCTGAGATC
  GTGCTCAAGAAGTGCTGCAAAATCCTCAATGGGGCGGGAGAGCCTCGTGTCTTCCGGCCCCGCGACCGGG
  ACGAGATGGTAAAGAAGGTGATTGAGCCCATGGCTTGCGATGGGCTCCGCACTATCTGCGTGGCCTACCG
  CGACTTCCCCAGCAGCCCGGAGCCGGACTGGGACAATGAGAATGACATCCTCAACGAACTCACCTGCATC
  TGCGTGGTGGGCATCGAGGACCCGGTGCGGCCAGAGGTCCCAGAAGCCATCCGCAAGTGCCAGCGGGCAG
  GCATCACGGTCCGCATGGTCACTGGCGACAATATCAACACGGCTCGGGCCATCGCCATCAAGTGTGGCAT
  CATCCATCCTGGGGAGGACTTTCTGTGCCTCGAGGGCAAGGAGTTCAACAGGAGGATCCGCAACGAGAAG
  GGGGAGATTGAGCAGGAGCGAATTGACAAGATCTGGCCAAAGCTGCGGGTGCTGGCTCGCTCCTCCCCAA
  CGGACAAGCATACCCTGGTTAAAGGCATCATCGACAGCACACACACTGAGCAGCGGCAGGTGGTGGCCGT
  GACGGGGGACGGGACCAACGACGGGCCTGCACTCAAGAAGGCCGACGTGGGCTTCGCCATGGGCATCGCA
  GGCACTGACGTGGCCAAGGAGGCCTCAGACATCATCCTGACAGACGACAATTTCAGCAGCATCGTCAAGG
  CAGTGATGTGGGGCCGCAACGTCTATGACAGCATCTCCAAATTCTTGCAGTTCCAGCTCACCGTCAACGT
  GGTGGCCGTGATTGTGGCCTTCACAGGCGCCTGCATCACGCAGGACTCCCCTCTGAAGGCCGTGCAGATG
  CTCTGGGTGAACCTCATCATGGACACGTTTGCCTCGCTGGCACTGGCCACTGAGCCGCCCACGGAGACCC
  TGCTGCTGAGGAAGCCGTACGGCCGCAACAAGCCGCTCATCTCCAGGACCATGATGAAGAACATCCTGGG
  CCATGCTGTCTACCAGCTTGCCCTCATCTTCACCCTGCTCTTTGTTGGCGAGAAGATGTTCCAGATCGAC
  AGCGGGAGGAACGCGCCCCTGCATTCGCCACCCTCAGAACATTACACCATCATCTTCAACACCTTCGTCA
  TGATGCAGCTCTTCAACGAGATCAACGCCCGCAAGATCCACGGCGAGCGCAATGTCTTTGACGGCATCTT
  CCGGAACCCCATCTTCTGCACCATCGTGCTGGGCACCTTTGCCATCCAGATAGTGATCGTGCAGTTTGGA
  GGGAAGCCATTCAGCTGCTCTCCACTGCAGCTGGACCAGTGGATGTGGTGCATATTCATTGGGTTAGGAG
  AGCTCGTTTGGGGCCAGGTCATCGCCACCATCCCGACCAGCAGACTCAAGTTCCTCAAGGAGGCAGGCAG
  GCTCACACAGAAGGAGGAGATCCCGGAGGAGGAGCTCAACGAGGACGTGGAGGAGATCGACCACGCGGAG
  CGGGAGCTGCGGCGGGGCCAGATCCTGTGGTTCCGAGGCCTGAATCGGATCCAGACACAGATCCGCGTCG
  TGAAGGCGTTCCGTAGCTCTCTCTATGAAGGTTTAGAAAAGCCTGAATCTCGAACCTCCATCCATAACTT
  CATGGCTCATCCTGAATTCCGGATCGAAGATTCCCAGCCCCACATCCCCCTCATTGATGACACCGACCTG
  GAAGAAGATGCCGCGCTCAAGCAGAACTCGAGCCCGCCGTCATCCCTCAACAAGAACAACAGCGCCATCG
  ACAGTGGGATCAACCTGACGACCGACACAAGCAAATCAGCTACCTCTTCAAGTCCAGGGAGCCCCATCCA
  CAGCCTGGAGACGTCGCTTTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001001331
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001331.2, NP_001001331.1
• RefSeq Size: 8962 bp
• RefSeq ORF: 3732 bp
• Locus ID: 491
• Cytogenetics: 3p25.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the longest isoform (1), also known as WB. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.