PAG3 (ASAP2) (NM_003887) Human Tagged ORF Clone

CAT#: RG221783

ASAP2 (GFP-tagged) - Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ASAP2
• Synonyms: AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha; SHAG1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221783 representing NM_003887
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGGACCAGATCTCCGTGTCGGAATTCGTGGCCGAGACCCATGAGGACTACAAGGCGCCCACGGCCT
  CCAGCTTCACCACCCGCACGGCGCAGTGCCGGAACACTGTGGCGGCCATCGAGGAGGCTTTGGACGTGGA
  CCGGATGGTTCTTTACAAAATGAAGAAATCCGTGAAAGCAATCAACAGCTCTGGGCTGGCTCACGTGGAA
  AATGAAGAGCAGTACACCCAGGCTCTGGAGAAGTTTGGCGGCAACTGTGTATGCAGAGATGACCCAGATT
  TAGGAAGTGCGTTCCTGAAGTTCTCAGTGTTTACAAAGGAGTTGACAGCACTTTTCAAAAACCTGATTCA
  GAATATGAACAACATAATCTCCTTCCCTTTGGACAGTTTGCTGAAGGGGGACCTGAAAGGAGTGAAAGGG
  GATCTGAAAAAGCCTTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAGATAGAAAAGGAGA
  AAAAGGAACACGCCAAGCTCCATGGGATGATTCGGACTGAAATAAGCGGAGCGGAAATTGCCGAAGAGAT
  GGAAAAGGAGAGGCGCTTCTTCCAGCTACAGATGTGCGAGTATCTGCTGAAGGTCAACGAAATCAAGATT
  AAAAAGGGAGTAGATTTACTTCAGAATCTGATCAAATACTTTCATGCCCAATGCAATTTTTTTCAGGATG
  GACTCAAAGCCGTGGAAAGCCTCAAACCTTCCATTGAAACGCTGTCTACGGATCTTCACACGATCAAACA
  GGCCCAGGATGAAGAAAGAAGGCAGTTGATACAGCTTCGAGATATTTTGAAATCCGCATTGCAGGTTGAA
  CAGAAAGAGTCTAGGAGAGACTCCCAAATTCGTCAGAGCACAGCTTATAGCTTACATCAGCCTCAGGGAA
  ACAAGGAACATGGGACCGAGCGGAACGGCAGCCTCTACAAGAAGAGTGACGGGATCCGAAAAGTGTGGCA
  GAAAAGGAAATGTTCAGTTAAAAATGGTTTTCTGACCATATCCCATGGTACCGCTAACCGGCCTCCTGCA
  AAGCTCAACCTGCTAACCTGCCAGGTGAAGACCAACCCTGAGGAGAAGAAGTGCTTTGACCTTATTTCAC
  ATGACAGAACTTACCACTTTCAAGCTGAAGATGAACAGGAATGTCAAATATGGATGTCTGTGCTGCAAAA
  TAGCAAAGAAGAAGCTTTAAACAATGCATTTAAGGGGGATGACAATACTGGAGAAAATAACATCGTCCAA
  GAACTGACAAAGGAGATCATCTCAGAAGTGCAGAGGATGACGGGCAATGACGTCTGCTGTGACTGTGGGG
  CGCCAGATCCTACATGGCTTTCCACCAACCTGGGCATCCTGACCTGCATCGAGTGTTCCGGAATCCACCG
  AGAGCTGGGGGTTCATTATTCCAGGATGCAGTCCCTGACCTTAGATGTACTGGGAACATCTGAGCTGCTG
  CTCGCCAAGAATATTGGGAATGCAGGCTTTAATGAGATCATGGAATGTTGCCTACCAGCTGAGGACTCAG
  TCAAACCCAACCCAGGCAGCGACATGAATGCAAGAAAGGACTACATCACAGCCAAGTACATCGAGAGGAG
  ATACGCAAGGAAGAAGCACGCGGATAACGCGGCGAAGCTTCACAGTCTTTGCGAGGCCGTCAAAACGAGA
  GATATTTTTGGATTGCTCCAAGCTTATGCTGATGGTGTGGATCTTACGGAAAAAATCCCACTGGCCAACG
  GACATGAGCCGGATGAAACGGCCCTCCACCTTGCAGTCAGATCCGTGGATCGAACCTCTCTTCACATTGT
  AGACTTTTTAGTTCAGAACAGTGGGAACCTGGATAAACAGACAGGGAAAGGCAGCACAGCCCTGCACTAC
  TGCTGCCTGACCGACAATGCCGAGTGCCTCAAGTTGCTCCTGCGGGGGAAGGCCTCCATCGAGATAGCAA
  ACGAGTCAGGAGAGACTCCGCTGGACATTGCCAAGCGCCTCAAGCACGAGCACTGTGAGGAGCTGCTGAC
  CCAAGCCTTATCTGGAAGATTTAATTCTCACGTTCACGTTGAATATGAATGGCGACTACTCCACGAAGAC
  CTGGATGAAAGTGATGACGACATGGATGAGAAATTGCAGCCCAGTCCCAACCGGCGGGAAGACCGGCCCA
  TCAGCTTCTACCAGCTGGGCTCCAACCAGCTTCAGTCTAACGCTGTATCTTTGGCCAGAGATGCTGCAAA
  CCTTGCCAAGGAGAAGCAGAGGGCTTTCATGCCCAGCATCTTGCAGAATGAGACTTACGGAGCCCTCCTG
  AGTGGCAGCCCACCTCCCGCCCAGCCTGCAGCCCCCAGCACCACCAGCGCCCCCCCGCTTCCTCCACGGA
  ATGTTGGCAAAGTTCAGACAGCCTCCTCTGCTAACACCCTGTGGAAGACAAACTCTGTAAGTGTGGACGG
  TGGAAGCCGGCAGCGATCTTCGTCAGATCCGCCAGCTGTCCATCCACCGCTGCCCCCTCTTCGCGTGACA
  TCTACCAATCCCCTGACCCCCACGCCGCCCCCACCCGTTGCCAAGACGCCCAGCGTAATGGAAGCCTTGA
  GCCAGCCGAGCAAGCCTGCCCCGCCTGGGATCTCACAGATCAGGCCCCCACCTCTGCCCCCACAGCCGCC
  CAGCCGCCTCCCGCAGAAGAAGCCTGCGCCGGGGGCTGACAAGTCCACCCCACTGACCAACAAAGGCCAA
  CCGAGAGGACCTGTGGATCTCTCTGCAACGGAAGCTCTGGGTCCTCTGTCCAATGCTATGGTCCTGCAGC
  CCCCTGCACCCATGCCTAGGAAGTCGCAGGCAACCAAGTTGAAGCCTAAGCGGGTGAAAGCGCTCTATAA
  CTGTGTGGCTGACAACCCCGATGAGCTCACCTTCTCCGAGGGGGATGTGATCATCGTGGACGGGGAGGAG
  GACCAGGAGTGGTGGATTGGCCACATTGATGGAGATCCTGGTCGCAAAGGCGCATTCCCGGTGTCATTTG
  TGCACTTTATCGCTGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221783 representing NM_003887
  Red=Cloning site Green=Tags(s)
  
  MPDQISVSEFVAETHEDYKAPTASSFTTRTAQCRNTVAAIEEALDVDRMVLYKMKKSVKAINSSGLAHVE
  NEEQYTQALEKFGGNCVCRDDPDLGSAFLKFSVFTKELTALFKNLIQNMNNIISFPLDSLLKGDLKGVKG
  DLKKPFDKAWKDYETKITKIEKEKKEHAKLHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKI
  KKGVDLLQNLIKYFHAQCNFFQDGLKAVESLKPSIETLSTDLHTIKQAQDEERRQLIQLRDILKSALQVE
  QKESRRDSQIRQSTAYSLHQPQGNKEHGTERNGSLYKKSDGIRKVWQKRKCSVKNGFLTISHGTANRPPA
  KLNLLTCQVKTNPEEKKCFDLISHDRTYHFQAEDEQECQIWMSVLQNSKEEALNNAFKGDDNTGENNIVQ
  ELTKEIISEVQRMTGNDVCCDCGAPDPTWLSTNLGILTCIECSGIHRELGVHYSRMQSLTLDVLGTSELL
  LAKNIGNAGFNEIMECCLPAEDSVKPNPGSDMNARKDYITAKYIERRYARKKHADNAAKLHSLCEAVKTR
  DIFGLLQAYADGVDLTEKIPLANGHEPDETALHLAVRSVDRTSLHIVDFLVQNSGNLDKQTGKGSTALHY
  CCLTDNAECLKLLLRGKASIEIANESGETPLDIAKRLKHEHCEELLTQALSGRFNSHVHVEYEWRLLHED
  LDESDDDMDEKLQPSPNRREDRPISFYQLGSNQLQSNAVSLARDAANLAKEKQRAFMPSILQNETYGALL
  SGSPPPAQPAAPSTTSAPPLPPRNVGKVQTASSANTLWKTNSVSVDGGSRQRSSSDPPAVHPPLPPLRVT
  STNPLTPTPPPPVAKTPSVMEALSQPSKPAPPGISQIRPPPLPPQPPSRLPQKKPAPGADKSTPLTNKGQ
  PRGPVDLSATEALGPLSNAMVLQPPAPMPRKSQATKLKPKRVKALYNCVADNPDELTFSEGDVIIVDGEE
  DQEWWIGHIDGDPGRKGAFPVSFVHFIAD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003887
• ORF Size: 3027 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003887.1, NP_003878.1
• RefSeq Size: 5711
• RefSeq ORF: 3021
• Locus ID: 8853
• Domains: ArfGap, SH3, PH, ANK
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• Gene Summary: This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]