PAG3 (ASAP2) (NM_003887) Human Untagged Clone

CAT#: SC117694

ASAP2 (untagged)-Human ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 (ASAP2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASAP2
• Synonyms: AMAP2; CENTB3; DDEF2; PAG3; PAP; Pap-alpha; SHAG1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003887 edited
  ATGCCGGACCAGATCTCCGTGTCGGAATTCGTGGCCGAGACCCATGAGGACTACAAGGCG
  CCCACGGCCTCCAGCTTCACCACCCGCACGGCGCAGTGCCGGAACACTGTGGCGGCCATC
  GAGGAGGCTTTGGACGTGGACCGGATGGTTCTTTACAAAATGAAGAAATCCGTGAAAGCA
  ATCAACAGCTCTGGGCTGGCTCACGTGGAAAATGAAGAGCAGTACACCCAGGCTCTGGAG
  AAGTTTGGCGGCAACTGTGTATGCAGAGATGACCCAGATTTAGGAAGTGCGTTCCTGAAG
  TTCTCAGTGTTTACAAAGGAGTTGACAGCACTTTTCAAAAACCTGATTCAGAATATGAAC
  AACATAATCTCCTTCCCTTTGGACAGTTTGCTGAAGGGGGACCTGAAAGGAGTGAAAGGG
  GATCTGAAAAAGCCTTTTGATAAAGCTTGGAAGGACTATGAAACAAAAATAACCAAGATA
  GAAAAGGAGAAAAAGGAACACGCCAAGCTCCATGGGATGATTCGGACTGAAATAAGCGGA
  GCGGAAATTGCCGAAGAGATGGAAAAGGAGAGGCGCTTCTTCCAGCTACAGATGTGCGAG
  TATCTGCTGAAGGTCAACGAAATCAAGATTAAAAAGGGAGTAGATTTACTTCAGAATCTG
  ATCAAATACTTTCATGCCCAATGCAATTTTTTTCAGGATGGACTCAAAGCCGTGGAAAGC
  CTCAAACCTTCCATTGAAACGCTGTCTACGGATCTTCACACGATCAAACAGGCCCAGGAT
  GAAGAAAGAAGGCAGTTGATACAGCTTCGAGATATTTTGAAATCCGCATTGCAGGTTGAA
  CAGAAAGAGTCTAGGAGAGACTCCCAAATTCGTCAGAGCACAGCTTATAGCTTACATCAG
  CCTCAGGGAAACAAGGAACATGGGACCGAGCGGAACGGCAGCCTCTACAAGAAGAGTGAC
  GGGATCCGAAAAGTGTGGCAGAAAAGGAAATGTTCAGTTAAAAATGGTTTTCTGACCATA
  TCCCATGGTACCGCTAACCGGCCTCCTGCAAAGCTCAACCTGCTAACCTGCCAGGTGAAG
  ACCAACCCTGAGGAGAAGAAGTGCTTTGACCTTATTTCACATGACAGAACTTACCACTTT
  CAAGCTGAAGATGAACAGGAATGTCAAATATGGATGTCTGTGCTGCAAAATAGCAAAGAA
  GAAGCTTTAAACAATGCATTTAAGGGGGATGACAATACTGGAGAAAATAACATCGTCCAA
  GAACTGACAAAGGAGATCATCTCAGAAGTGCAGAGGATGACGGGCAATGACGTCTGCTGT
  GACTGTGGGGCGCCAGATCCTACATGGCTTTCCACCAACCTGGGCATCCTGACCTGCATC
  GAGTGTTCCGGAATCCACCGAGAGCTGGGGGTTCATTATTCCAGGATGCAGTCCCTGACC
  TTAGATGTACTGGGAACATCTGAGCTGCTGCTCGCCAAGAATATTGGGAATGCAGGCTTT
  AATGAGATCATGGAATGTTGCCTACCAGCTGAGGACTCAGTCAAACCCAACCCAGGCAGC
  GACATGAATGCAAGAAAGGACTACATCACAGCCAAGTACATCGAGAGGAGATACGCAAGG
  AAGAAGCACGCGGATAACGCGGCGAAGCTTCACAGTCTTTGCGAGGCCGTCAAAACGAGA
  GATATTTTTGGATTGCTCCAAGCTTATGCTGATGGTGTGGATCTTACGGAAAAAATCCCA
  CTGGCCAACGGACATGAGCCGGATGAAACGGCCCTCCACCTTGCAGTCAGATCCGTGGAT
  CGAACCTCTCTTCACATTGTAGACTTTTTAGTTCAGAACAGTGGGAACCTGGATAAACAG
  ACAGGGAAAGGCAGCACAGCCCTGCACTACTGCTGCCTGACCGACAATGCCGAGTGCCTC
  AAGTTGCTCCTGCGGGGGAAGGCCTCCATCGAGATAGCAAACGAGTCAGGAGAGACTCCG
  CTGGACATTGCCAAGCGCCTCAAGCACGAGCACTGTGAGGAGCTGCTGACCCAAGCCTTA
  TCTGGAAGATTTAATTCTCACGTTCACGTTGAATATGAATGGCGACTACTCCACGAAGAC
  CTGGATGAAAGTGATGACGACATGGATGAGAAATTGCAGCCCAGTCCCAACCGGCGGGAA
  GACCGGCCCATCAGCTTCTACCAGCTGGGCTCCAACCAGCTTCAGTCTAACGCTGTATCT
  TTGGCCAGAGATGCTGCAAACCTTGCCAAGGAGAAGCAGAGGGCTTTCATGCCCAGCATC
  TTGCAGAATGAGACTTACGGAGCCCTCCTGAGTGGCAGCCCACCTCCCGCCCAGCCTGCA
  GCCCCCAGCACCACCAGCGCCCCCCCGCTTCCTCCACGGAATGTTGGCAAAGTTCAGACA
  GCCTCCTCTGCTAACACCCTGTGGAAGACAAACTCTGTAAGTGTGGACGGTGGAAGCCGG
  CAGCGATCTTCGTCAGATCCGCCAGCTGTCCATCCACCGCTGCCCCCTCTTCGCGTGACA
  TCTACCAATCCCCTGACCCCCACGCCGCCCCCACCCGTTGCCAAGACGCCCAGCGTAATG
  GAAGCCTTGAGCCAGCCGAGCAAGCCTGCCCCGCCTGGGATCTCACAGATCAGGCCCCCA
  CCTCTGCCCCCACAGCCGCCCAGCCGCCTCCCGCAGAAGAAGCCTGCGCCGGGGGCTGAC
  AAGTCCACCCCACTGACCAACAAAGGCCAACCGAGAGGACCTGTGGATCTCTCTGCAACG
  GAAGCTCTGGGTCCTCTGTCCAATGCTATGGTCCTGCAGCCCCCTGCACCCATGCCTAGG
  AAGTCGCAGGCAACCAAGTTGAAGCCTAAGCGGGTGAAAGCGCTCTATAACTGTGTGGCT
  GACAACCCCGATGAGCTCACCTTCTCCGAGGGGGATGTGATCATCGTGGACGGGGAGGAG
  GACCAGGAGTGGTGGATTGGCCACATTGATGGAGATCCTGGTCGCAAAGGCGCATTCCCG
  GTGTCATTTGTGCACTTTATCGCTGACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003887 unedited
  GGGCGGCCGCGTAATTCGCACGAGGGCCCTCGGCGCGCAGGCGGGCGGACCGGCCGAGCT
  GCGCGGGGCTGCGCGCCGCCCCTGCTCCGCCGCCAGGCCCCGCGCGGCTCCCGCGCCCGG
  CGCTCCCCTTTGTCCGCGGGCCGGAGCGGCGGCGGCAGCGGCGGTGTCCGAGCGGCGGTC
  GGAGCCTGCTGCGGCAGTTGAGGCGGCGGCGCCCCTGCGGCTGTGCGCCAGCGCCCTCGC
  GCCGAGGCGATGCCGGACCAGATCTCCGTGTCGGAATTCGTGGCCGAGACCCATGAAGAC
  TACAAGGCGCCCACGGCCTCCAGCTTCACCACCCGCACGGCGCAGTGCCGGAACACTGTG
  GCGGCCATCGAGGAGGCTTTGGACGTGGACCGGATGGTTCTTTACAAAATGAAGAAATCC
  GTGAAAGCAATCAACAGCTCTGGGCTGGCTCACGTGGAAAATGAAGAGCAGTACACCCAG
  GCTCTGGAGAAGTTTGGCGGCAACTGTGTATGCAGAGATGACCCAGATTTAGGAAGTGCG
  TTCCTGAAGTTCTCAGTGTTTACAAAGGGAGTGACAGCACTTTTCACAAACCTGATTCAG
  AATATGAACAACATAATCTCCTTCCCTTTGGACAGTTTGCTGAATGGGGACCTGAATAGA
  GTGAAAGGGGATCTGAAAAAGCCTTTTGNATAAGCCTGGGAGGACTATGAAACANAAATT
  ACCCAGATCAGAAACGGAGAAAAGGGACACGCCAAGCTCCATGGGATGATTCGGACTGAA
  ATAAGCCGAGCGGAAATTGCCGATAGATGGAAAAGGAGAGCGCTTTCTTCAGCTACAGAT
  GTGCGAGTATCTGCTGAAGGTCACGAAATCCAGATTAAGAAGGAAGTAGATTTACTTCAA
  AATCCGATCAAATACTTTCATGCCCATGCAATTTTTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003887 unedited
  CTTTGGCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTCCCATTAAAAAGAATTTTATTTTTTTCCAGTCAAATGACTAGTTAACAAAAAAA
  GTAACCTTATTAAACATGCTCTAATTATAAATCACTGCTTTAAGGACAATGAAAATAATC
  AATTTCGGTTATACAATATATACAGTTGGGCTGCACCCAAAGTAATCAGGGGAATGAACT
  GAATATCATACATCTAAAAATAGCATCCTAAGCTGCATATTATGTTATCCACCCCTTAAC
  AAATCACACAGTTACTTTAAGTCTGGGACCATGTTCTGACCCATCATCCCAAATCTGATG
  GAAAATGGCATGCAAAATGCCAAAATCCTGCAGCTGCAGTTCATGAAACATAAACTTTAA
  ATATAAATAAATATCTACAAGGTTTTTCTTTCTCTTAGTTGCTTTTTTAATTGGCAGGGA
  GCAAATACCTAAAAAGGGATTTTACCAGGGCCGTTAATATAATTTCTCCTCTGGAAAAAG
  TACTATTAGTTACTGCACAATAGCACCAAATTGTAAACTGGAAAAATATTTCCTAGGTAT
  TTATGTCCCAGGGACCCTGACAAATTATTTGGGGACTGGAGTTCTAATGTTGATATGAAT
  CAACTTTATTCCTTATATAGCTGGCACCAGGTGGTCAAAAGGCTGCCTATAAAAACAGAT
  GCAGGAGGATGGTTAAAATAGACATAAGGGCCCTGTGCAAAACACATCTGGTTACTTAAA
  TCTATGAAAAAATAATGGGAGGGTTCAAACGAAATAACATTAGTGAAACCAGATGTGGTG
  CTTAAAAAAAACAAAACCAAACCTCCGATTTACCTTTTTCCCGGACCTTTGAGGTAAAAG
  CGATGCTTACACATATAAAATTTCCGTTTGTTTGTTTCCTTTACAAAAAAAAGACAGCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003887
• ORF Size: 3021 bp
• Insert Size: 5270
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_003887.1, NP_003878.1
• RefSeq Size: 5711
• RefSeq ORF: 3021
• Locus ID: 8853
• Domains: ArfGap, SH3, PH, ANK
• Protein Pathways: Endocytosis, Fc gamma R-mediated phagocytosis
• Gene Summary: This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).