KIAA1970 (EARS2) (NM_001083614) Human Tagged ORF Clone

CAT#: RG221871

EARS2 (GFP-tagged) - Human glutamyl-tRNA synthetase 2, mitochondrial (putative) (EARS2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EARS2
• Synonyms: COXPD12; gluRS; MSE1; mtGlnRS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221871 representing NM_001083614
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCTCCTGAGGAGACTGCTGCAGCGCGAGAGGCCTTCGGCGGCCTCTGGCCGCCCCGTAGGAC
  GGCGCGAGGCCAACCTGGGCACTGATGCCGGGGTTGCGGTGCGAGTGCGGTTCGCTCCCAGCCCCACAGG
  CTTCTTGCACCTGGGTGGCCTCCGCACTGCCTTGTACAACTACATCTTTGCTAAGAAGTACCAGGGGAGC
  TTCATCCTGAGGCTAGAGGACACAGATCAGACTCGCGTTGTGCCTGGGGCAGCGGAGAATATTGAGGACA
  TGCTGGAGTGGGCAGGCATCCCGCCTGATGAGAGCCCCCGCCGGGGCGGTCCTGCTGGGCCCTACCAGCA
  ATCTCAGCGGTTGGAGCTGTATGCCCAGGCCACAGAAGCGCTGCTGAAGACCGGAGCTGCTTACCCCTGT
  TTCTGCTCACCCCAGCGGCTGGAGCTCCTGAAGAAGGAGGCCTTGCGGAACCACCAGACGCCCCGGTATG
  ACAATCGGTGCAGGAACATGAGCCAGGAGCAGGTGGCCCAGAAGCTGGCCAAGGACCCCAAGCCTGCGAT
  CCGCTTCCGCCTGGAGCAGGTGGTGCCAGCCTTCCAGGACCTGGTCTATGGCTGGAATAGGCATGAAGTG
  GCCAGCGTGGAGGGAGACCCAGTCATCATGAAGAGCGACGGCTTCCCCACATACCACCTGGCCTGCGTGG
  TGGACGACCACCACATGGGCATCAGCCACGTGCTGCGAGGCTCTGAGTGGCTCGTCTCCACTGCCAAGCA
  CCTGCTCCTCTACCAGGCCCTGGGCTGGCAGCCACCCCACTTCGCCCACCTGCCCCTGCTCCTCAACAGG
  GATGGCAGCAAGCTCTCCAAGAGGCAAGGGGACGTTTTCCTGGAGCACTTTGCTGCTGATGGCTTCCTGC
  CCGATTCCTTGTTGGACATCATCACCAACTGTGGCTCAGGTTTTGCAGAGAACCAAATGGGCAGGACCCT
  GCCGGAGCTGATCACACAGTTCAACCTGACACAGGTCACCTGTCACTCAGCCCTGCTGGACCTGGAGAAG
  CTCCCAGAATTCAACAGACTGCACCTCCAGCGGCTGGTGAGCAATGAGAGCCAGAGGCGCCAGCTGGTGG
  GGAAGCTGCAGGTCCTTGTGGAGGAGGCCTTTGGTTGCCAGCTGCAAAACAGGGATGTCCTCAACCCAGT
  CTACGTGGAGAGGATCCTCCTGCTGAGACAGGGTCACATTTGCCGCCTGCAGGACTTGGTGTCCCCAGTA
  TACTCTTACCTGTGGACTCGCCCTGCAGTAGGTCGAGCACAGCTGGACGCCATCTCGGAGAAGGTGGATG
  TGATTGCCAAGCGTGTGCTGGGGCTTCTAGAAAGATCTAGTATGAGCTTAACTCAGGATATGCTGAATGG
  AGAACTGAAGAAGCTATCAGAAGGTCTGGAAGGCACCAAGTACAGTAATGTGATGAAACTCCTTCGGATG
  GCCCTCAGTGGACAGCAGCAAGGACCTCCTGTAGCTGAGATGATGTTGGCCTTGGGACCAAAGGAAGTAC
  GGGAACGGATCCAGAAGGTGGTTTCCAGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221871 representing NM_001083614
  Red=Cloning site Green=Tags(s)
  
  MAALLRRLLQRERPSAASGRPVGRREANLGTDAGVAVRVRFAPSPTGFLHLGGLRTALYNYIFAKKYQGS
  FILRLEDTDQTRVVPGAAENIEDMLEWAGIPPDESPRRGGPAGPYQQSQRLELYAQATEALLKTGAAYPC
  FCSPQRLELLKKEALRNHQTPRYDNRCRNMSQEQVAQKLAKDPKPAIRFRLEQVVPAFQDLVYGWNRHEV
  ASVEGDPVIMKSDGFPTYHLACVVDDHHMGISHVLRGSEWLVSTAKHLLLYQALGWQPPHFAHLPLLLNR
  DGSKLSKRQGDVFLEHFAADGFLPDSLLDIITNCGSGFAENQMGRTLPELITQFNLTQVTCHSALLDLEK
  LPEFNRLHLQRLVSNESQRRQLVGKLQVLVEEAFGCQLQNRDVLNPVYVERILLLRQGHICRLQDLVSPV
  YSYLWTRPAVGRAQLDAISEKVDVIAKRVLGLLERSSMSLTQDMLNGELKKLSEGLEGTKYSNVMKLLRM
  ALSGQQQGPPVAEMMLALGPKEVRERIQKVVSS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001083614
• ORF Size: 1569 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001083614.1, NP_001077083.1
• RefSeq Size: 3979
• RefSeq ORF: 1572
• Locus ID: 124454
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Metabolic pathways, Porphyrin and chlorophyll metabolism
• Gene Summary: This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of glutamate to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 12 (COXPD12). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]