KIAA1970 (EARS2) (NM_001083614) Human Untagged Clone

CAT#: SC316061

EARS2 (untagged)-Human glutamyl-tRNA synthetase 2, mitochondrial (putative) (EARS2), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EARS2
• Synonyms: COXPD12; gluRS; MSE1; mtGlnRS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001083614, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGCTCCTGAGGAGACTGCTGCAGCGCGAGAGGCCTTCGGCGGCCTCTGGCCGCCCCGTAGGAC
  GGCGCGAGGCCAACCTGGGCACTGATGCCGGGGTTGCGGTGCGAGTGCGGTTCGCTCCCAGCCCCACAGG
  CTTCTTGCACCTGGGTGGCCTCCGCACTGCCTTGTACAACTACATCTTTGCTAAGAAGTACCAGGGGAGC
  TTCATCCTGAGGCTAGAGGACACAGATCAGACTCGCGTTGTGCCTGGGGCAGCGGAGAATATTGAGGACA
  TGCTGGAGTGGGCAGGCATCCCGCCTGATGAGAGCCCCCGCCGGGGCGGTCCTGCTGGGCCCTACCAGCA
  ATCTCAGCGGTTGGAGCTGTATGCCCAGGCCACAGAAGCGCTGCTGAAGACCGGAGCTGCTTACCCCTGT
  TTCTGCTCACCCCAGCGGCTGGAGCTCCTGAAGAAGGAGGCCTTGCGGAACCACCAGACGCCCCGGTATG
  ACAATCGGTGCAGGAACATGAGCCAGGAGCAGGTGGCCCAGAAGCTGGCCAAGGACCCCAAGCCTGCGAT
  CCGCTTCCGCCTGGAGCAGGTGGTGCCAGCCTTCCAGGACCTGGTCTATGGCTGGAATAGGCATGAAGTG
  GCCAGCGTGGAGGGAGACCCAGTCATCATGAAGAGCGACGGCTTCCCCACATACCACCTGGCCTGCGTGG
  TGGACGACCACCACATGGGCATCAGCCACGTGCTGCGAGGCTCTGAGTGGCTCGTCTCCACTGCCAAGCA
  CCTGCTCCTCTACCAGGCCCTGGGCTGGCAGCCACCCCACTTCGCCCACCTGCCCCTGCTCCTCAACAGG
  GATGGCAGCAAGCTCTCCAAGAGGCAAGGGGACGTTTTCCTGGAGCACTTTGCTGCTGATGGCTTCCTGC
  CCGATTCCTTGTTGGACATCATCACCAACTGTGGCTCAGGTTTTGCAGAGAACCAAATGGGCAGGACCCT
  GCCGGAGCTGATCACACAGTTCAACCTGACACAGGTCACCTGTCACTCAGCCCTGCTGGACCTGGAGAAG
  CTCCCAGAATTCAACAGACTGCACCTCCAGCGGCTGGTGAGCAATGAGAGCCAGAGGCGCCAGCTGGTGG
  GGAAGCTGCAGGTCCTTGTGGAGGAGGCCTTTGGTTGCCAGCTGCAAAACAGGGATGTCCTCAACCCAGT
  CTACGTGGAGAGGATCCTCCTGCTGAGACAGGGTCACATTTGCCGCCTGCAGGACTTGGTGTCCCCAGTA
  TACTCTTACCTGTGGACTCGCCCTGCAGTAGGTCGAGCACAGCTGGACGCCATCTCGGAGAAGGTGGATG
  TGATTGCCAAGCGTGTGCTGGGGCTTCTAGAAAGATCTAGTATGAGCTTAACTCAGGATATGCTGAATGG
  AGAACTGAAGAAGCTATCAGAAGGTCTGGAAGGCACCAAGTACAGTAATGTGATGAAACTCCTTCGGATG
  GCCCTCAGTGGACAGCAGCAAGGACCTCCTGTAGCTGAGATGATGTTGGCCTTGGGACCAAAGGAAGTAC
  GGGAACGGATCCAGAAGGTGGTTTCCAGCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001083614
• ORF Size: 1572 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001083614.1, NP_001077083.1
• RefSeq Size: 3979
• RefSeq ORF: 1572
• Locus ID: 124454
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Metabolic pathways, Porphyrin and chlorophyll metabolism
• Gene Summary: This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. This protein is encoded by the nuclear genome but is likely to be imported to the mitochondrion where it is thought to catalyze the ligation of glutamate to tRNA molecules. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency 12 (COXPD12). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.