ZAK (NM_016653) Human Tagged ORF Clone

CAT#: RG221970

ZAK (GFP-tagged) - Human sterile alpha motif and leucine zipper containing kinase AZK (ZAK), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZAK
• Synonyms: AZK; CNM6; MLK7; mlklak; MLT; MLTK; MLTKalpha; MLTKbeta; MRK; pk; SFMMP; ZAK
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221970 representing NM_016653
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGTCTCTCGGTGCCTCCTTTGTGCAAATTAAATTTGATGACTTGCAGTTTTTTGAAAACTGCGGTG
  GAGGAAGTTTTGGGAGTGTTTATCGAGCCAAATGGATATCACAGGACAAGGAGGTGGCTGTAAAGAAGCT
  CCTCAAAATAGAGAAAGAGGCAGAAATACTCAGTGTCCTCAGTCACAGAAACATCATCCAGTTTTATGGA
  GTAATTCTTGAACCTCCCAACTATGGCATTGTCACAGAATATGCTTCTCTGGGATCACTCTATGATTACA
  TTAACAGTAACAGAAGTGAGGAGATGGATATGGATCACATTATGACCTGGGCCACTGATGTAGCCAAAGG
  AATGCATTATTTACATATGGAGGCTCCTGTCAAGGTGATTCACAGAGACCTCAAGTCAAGAAACGTTGTT
  ATAGCTGCTGATGGAGTATTGAAGATCTGTGACTTTGGTGCCTCTCGGTTCCATAACCATACAACACACA
  TGTCCTTGGTTGGAACTTTCCCATGGATGGCTCCAGAAGTTATCCAGAGTCTCCCTGTGTCAGAAACTTG
  TGACACATATTCCTATGGTGTGGTTCTCTGGGAGATGCTAACAAGGGAGGTCCCCTTTAAAGGTTTGGAA
  GGATTACAAGTAGCTTGGCTTGTAGTGGAAAAAAACGAGAGATTAACCATTCCAAGCAGTTGCCCCAGAA
  GTTTTGCTGAACTGTTACATCAGTGTTGGGAAGCTGATGCCAAGAAACGGCCATCATTCAAGCAAATCAT
  TTCAATCCTGGAGTCCATGTCAAATGACACGAGCCTTCCTGACAAGTGTAACTCATTCCTACACAACAAG
  GCGGAGTGGAGGTGCGAAATTGAGGCAACTCTTGAGAGGCTAAAGAAACTAGAGCGTGATCTCAGCTTTA
  AGGAGCAGGAGCTTAAAGAACGAGAAAGACGTTTAAAGATGTGGGAGCAAAAGCTGACAGAGCAGTCCAA
  CACCCCGCTGCTGCCTTCCTTTGAGATTGGTGCATGGACGGAAGACGATGTGTATTGTTGGGTTCAGCAG
  CTCGTCAGAAAAGGTGACTCTTCAGCAGAGATGAGTGTATATGCAAGCTTGTTTAAAGAAAACAACATTA
  CAGGGAAGCGGCTGCTGCTGCTGGAGGAAGAAGACCTGAAAGACATGGGCATTGTCTCCAAGGGGCATAT
  CATTCACTTCAAGTCAGCCATTGAGAAATTAACCCATGATTACATAAATTTGTTTCACTTCCCACCACTA
  ATTAAGGACTCAGGAGGTGAACCTGAAGAAAATGAGGAAAAAATAGTGAACCTGGAACTGGTTTTTGGTT
  TTCACTTGAAACCAGGAACTGGCCCACAGGATTGTAAGTGGAAAATGTATATGGAGATGGATGGGGATGA
  AATTGCAATAACCTACATAAAAGATGTGACATTCAACACTAACCTACCTGATGCGGAGATTTTAAAGATG
  ACAAAGCCACCATTTGTAATGGAGAAGTGGATTGTAGGAATAGCAAAAAGTCAGACTGTGGAGTGCACTG
  TCACATATGAGAGTGATGTTAGAACTCCAAAAAGCACTAAACATGTCCATTCGATTCAGTGGAGTAGAAC
  AAAACCTCAGGATGAAGTGAAAGCAGTCCAACTTGCCATTCAGACATTATTCACCAATTCAGATGGCAAC
  CCTGGAAGCAGGTCCGACTCAAGTGCTGATTGCCAGTGGTTAGATACTCTGAGGATGCGGCAGATTGCAT
  CCAACACTTCTTTACAGCGTTCCCAGAGCAATCCTATTCTGGGGTCACCGTTCTTCTCACACTTTGATGG
  CCAGGATTCCTACGCTGCTGCTGTGAGACGGCCCCAGGTGCCCATTAAGTATCAACAGATTACACCTGTG
  AACCAGTCCAGAAGCTCGTCTCCTACTCAGTATGGACTGACCAAAAACTTCTCTTCCCTACATCTCAACT
  CTAGGGACAGTGGCTTTTCCAGTGGCAATACTGACACCTCTTCAGAGAGGGGTCGATACTCAGACAGAAG
  CAGGAACAAATATGGACGTGGTAGTATATCACTCAATTCTTCTCCTAGAGGAAGATACAGTGGAAAGAGT
  CAGCATTCCACTCCTTCAAGAGGAAGATACCCTGGAAAGTTCTACAGGGTTTCTCAGTCAGCACTCAATC
  CTCACCAGTCGCCTGACTTCAAGAGAAGCCCCAGGGACCTCCACCAACCCAACACCATACCAGGGATGCC
  TTTGCACCCTGAGACTGACTCAAGAGCCAGTGAAGAGGACAGCAAAGTCAGCGAAGGGGGCTGGACAAAA
  GTGGAATACCGGAAAAAGCCCCACAGGCCATCTCCCGCCAAAACCAATAAAGAGAGAGCCAGAGGGGACC
  ACCGTGGATGGAGAAACTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221970 representing NM_016653
  Red=Cloning site Green=Tags(s)
  
  MSSLGASFVQIKFDDLQFFENCGGGSFGSVYRAKWISQDKEVAVKKLLKIEKEAEILSVLSHRNIIQFYG
  VILEPPNYGIVTEYASLGSLYDYINSNRSEEMDMDHIMTWATDVAKGMHYLHMEAPVKVIHRDLKSRNVV
  IAADGVLKICDFGASRFHNHTTHMSLVGTFPWMAPEVIQSLPVSETCDTYSYGVVLWEMLTREVPFKGLE
  GLQVAWLVVEKNERLTIPSSCPRSFAELLHQCWEADAKKRPSFKQIISILESMSNDTSLPDKCNSFLHNK
  AEWRCEIEATLERLKKLERDLSFKEQELKERERRLKMWEQKLTEQSNTPLLPSFEIGAWTEDDVYCWVQQ
  LVRKGDSSAEMSVYASLFKENNITGKRLLLLEEEDLKDMGIVSKGHIIHFKSAIEKLTHDYINLFHFPPL
  IKDSGGEPEENEEKIVNLELVFGFHLKPGTGPQDCKWKMYMEMDGDEIAITYIKDVTFNTNLPDAEILKM
  TKPPFVMEKWIVGIAKSQTVECTVTYESDVRTPKSTKHVHSIQWSRTKPQDEVKAVQLAIQTLFTNSDGN
  PGSRSDSSADCQWLDTLRMRQIASNTSLQRSQSNPILGSPFFSHFDGQDSYAAAVRRPQVPIKYQQITPV
  NQSRSSSPTQYGLTKNFSSLHLNSRDSGFSSGNTDTSSERGRYSDRSRNKYGRGSISLNSSPRGRYSGKS
  QHSTPSRGRYPGKFYRVSQSALNPHQSPDFKRSPRDLHQPNTIPGMPLHPETDSRASEEDSKVSEGGWTK
  VEYRKKPHRPSPAKTNKERARGDHRGWRNF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016653
• ORF Size: 2400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016653.1, NP_057737.1
• RefSeq Size: 3767
• RefSeq ORF: 2403
• Locus ID: 51776
• Domains: pkinase, TyrKc, SAM, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway, Tight junction
• Gene Summary: This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]