Contactin 3 (CNTN3) (NM_020872) Human Tagged ORF Clone

CAT#: RG221979

CNTN3 (GFP-tagged) - Human contactin 3 (plasmacytoma associated) (CNTN3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CNTN3
• Synonyms: BIG-1; PANG; PCS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221979 representing NM_020872
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGTTTCCATGGAAACAGTTGATCCTGCTTTCATTCATTGGCTGCTTAGGAGGTGAGCTTCTCTTAC
  AAGGCCCTGTATTTATCAAAGAACCCAGCAACAGCATTTTCCCTGTTGGTTCAGAAGATAAAAAAATAAC
  TTTGCATTGTGAAGCAAGAGGCAATCCATCACCTCATTACAGATGGCAGCTGAATGGAAGTGATATTGAT
  ATGAGTATGGAACATCGTTATAAGTTGAATGGAGGAAATCTTGTGGTTATTAATCCCAACAGAAATTGGG
  ATACAGGAACTTACCAATGTTTTGCAACAAATTCACTTGGAACAATTGTCAGCAGAGAAGCCAAACTTCA
  GTTTGCCTATCTTGAAAATTTTAAAACCAAAATGAGGAGTACAGTGTCTGTGCGTGAAGGCCAGGGAGTT
  GTGCTGCTCTGCGGCCCCCCACCACACTCTGGAGAACTGTCATATGCTTGGATCTTCAATGAATACCCAT
  CGTTTGTTGAAGAAGATAGTCGGAGATTTGTCTCCCAGGAGACAGGGCACCTCTACATATCTAAGGTGGA
  GCCGTCTGATGTGGGAAATTACACATGTGTGGTGACAAGTATGGTGACAAATGCCCGAGTGCTGGGCTCT
  CCAACTCCTTTGGTGCTACGTTCTGATGGTGTGATGGGTGAATATGAACCTAAAATAGAAGTTCAGTTTC
  CAGAAACTCTTCCAGCAGCTAAAGGTTCGACTGTGAAATTGGAATGTTTTGCCCTTGGAAATCCCATACC
  TCAGATTAATTGGAGAAGAAGTGATGGGCTGCCATTTTCCAGCAAAATTAAATTAAGGAAGTTCAGTGGT
  GTGCTTGAAATCCCCAACTTCCAACAGGAAGATGCAGGTTCCTATGAATGCATTGCTGAGAATTCACGAG
  GAAAAAATGTTGCCAGAGGGCGTCTCACTTACTATGCAAAGCCCCATTGGGTTCAACTCATAAAGGATGT
  GGAAATAGCCGTGGAGGACAGTCTTTATTGGGAATGCAGGGCAAGCGGCAAGCCCAAGCCTTCCTACCGA
  TGGCTGAAAAATGGAGCAGCCCTGGTGCTAGAGGAGAGAACACAGATAGAAAATGGTGCCCTTACAATAT
  CAAACCTAAGTGTGACTGATTCTGGCATGTTCCAGTGCATAGCAGAAAACAAACATGGCCTTGTTTATTC
  CAGTGCTGAGCTCAAAGTTGTTGCTTCTGCTCCAGATTTTTCAAAGAATCCAATGAAGAAGTTGGTTCAG
  GTGCAGGTGGGCAGCCTGGTCAGCTTGGATTGTAAACCCAGAGCCTCCCCAAGGGCACTCTCTTCCTGGA
  AGAAGGGGGATGTGAGCGTGCAGGAGCATGAAAGAATTTCTTTGTTAAACGATGGAGGACTCAAAATAGC
  CAATGTGACTAAAGCTGATGCTGGAACTTACACCTGCATGGCAGAAAACCAGTTTGGGAAAGCAAATGGC
  ACAACACATTTGGTTGTTACGGAACCAACAAGAATAACTTTGGCACCATCTAACATGGATGTTTCTGTTG
  GTGAAAGCGTCATATTGCCCTGCCAGGTACAACATGACCCGCTGTTAGACATCATCTTTACCTGGTATTT
  CAATGGGGCCCTTGCAGATTTTAAGAAAGATGGATCTCACTTTGAGAAAGTTGGTGGGAGTTCATCTGGT
  GATTTAATGATCAGAAACATTCAGCTGAAACACAGTGGGAAATATGTTTGTATGGTGCAAACGGGGGTGG
  ACAGTGTTTCATCTGCTGCTGACCTCATAGTAAGAGGTTCACCTGGACCACCAGAAAATGTGAAGGTAGA
  TGAAATTACAGACACAACAGCCCAACTCTCTTGGAAAGAAGGTAAAGACAACCATAGCCCAGTTATATCC
  TATTCTATCCAGGCTCGGACACCTTTCTCCGTGGGTTGGCAAACCGTCACAACAGTGCCTGAGGTCATCG
  ATGGGAAGACGCACACAGCCACTGTAGTTGAGTTAAACCCATGGGTGGAATATGAATTTCGGGTTGTAGC
  CAGTAACAAAATTGGAGGTGGAGAACCAAGTTTACCCTCAGAAAAAGTAAGAACTGAAGAGGCAGTTCCA
  GAAGTGCCTCCTTCTGAAGTCAATGGAGGAGGCGGAAGCCGGTCTGAACTTGTGATAACCTGGGATCCAG
  TCCCTGAAGAACTACAGAATGGTGAAGGTTTTGGGTATGTTGTTGCTTTCCGCCCTCTTGGGGTTACCAC
  CTGGATCCAGACAGTGGTGACATCCCCTGACACCCCAAGATATGTCTTTAGGAATGAAAGCATCGTGCCA
  TATTCACCATATGAAGTTAAAGTGGGTGTTTATAATAACAAAGGTGAAGGACCATTTAGCCCAGTGACAA
  CAGTGTTCTCTGCAGAAGAAGAGCCTACAGTGGCCCCATCTCAAGTCTCTGCAAATAGCCTATCTTCCTC
  AGAAATTGAGGTTTCATGGAACACCATTCCTTGGAAGTTGAGCAATGGACATTTACTGGGCTATGAGGTG
  CGGTACTGGAATGGGGGTGGAAAGGAGGAATCATCCAGTAAGATGAAAGTGGCAGGAAATGAGACATCAG
  CCAGACTACGGGGCCTGAAGAGCAACCTGGCCTATTACACGGCTGTCCGGGCTTACAACAGTGCCGGCGC
  TGGGCCTTTTAGCGCCACAGTTAATGTAACCACCAAGAAAACGCCTCCCAGTCAGCCACCAGGAAATGTT
  GTTTGGAATGCCACAGACACTAAAGTGTTACTTAATTGGGAGCAAGTTAAAGCCATGGAGAATGAGTCAG
  AAGTAACAGGATATAAAGTTTTCTATAGGACTAGCAGTCAAAATAACGTACAAGTACTGAACACAAATAA
  AACTTCAGCTGAACTTGTGCTGCCCATTAAAGAGGACTACATTATTGAAGTCAAGGCCACAACAGATGGA
  GGGGATGGGACCAGTAGTGAACAGATCAGGATTCCACGAATAACCAGTATGGATGCAAGAGGATCCACTT
  CAGCCATCTCGAATGTCCACCCTATGTCAAGTTATATGCCTATAGTACTGTTCTTAATTGTATATGTCCT
  GTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221979 representing NM_020872
  Red=Cloning site Green=Tags(s)
  
  MMFPWKQLILLSFIGCLGGELLLQGPVFIKEPSNSIFPVGSEDKKITLHCEARGNPSPHYRWQLNGSDID
  MSMEHRYKLNGGNLVVINPNRNWDTGTYQCFATNSLGTIVSREAKLQFAYLENFKTKMRSTVSVREGQGV
  VLLCGPPPHSGELSYAWIFNEYPSFVEEDSRRFVSQETGHLYISKVEPSDVGNYTCVVTSMVTNARVLGS
  PTPLVLRSDGVMGEYEPKIEVQFPETLPAAKGSTVKLECFALGNPIPQINWRRSDGLPFSSKIKLRKFSG
  VLEIPNFQQEDAGSYECIAENSRGKNVARGRLTYYAKPHWVQLIKDVEIAVEDSLYWECRASGKPKPSYR
  WLKNGAALVLEERTQIENGALTISNLSVTDSGMFQCIAENKHGLVYSSAELKVVASAPDFSKNPMKKLVQ
  VQVGSLVSLDCKPRASPRALSSWKKGDVSVQEHERISLLNDGGLKIANVTKADAGTYTCMAENQFGKANG
  TTHLVVTEPTRITLAPSNMDVSVGESVILPCQVQHDPLLDIIFTWYFNGALADFKKDGSHFEKVGGSSSG
  DLMIRNIQLKHSGKYVCMVQTGVDSVSSAADLIVRGSPGPPENVKVDEITDTTAQLSWKEGKDNHSPVIS
  YSIQARTPFSVGWQTVTTVPEVIDGKTHTATVVELNPWVEYEFRVVASNKIGGGEPSLPSEKVRTEEAVP
  EVPPSEVNGGGGSRSELVITWDPVPEELQNGEGFGYVVAFRPLGVTTWIQTVVTSPDTPRYVFRNESIVP
  YSPYEVKVGVYNNKGEGPFSPVTTVFSAEEEPTVAPSQVSANSLSSSEIEVSWNTIPWKLSNGHLLGYEV
  RYWNGGGKEESSSKMKVAGNETSARLRGLKSNLAYYTAVRAYNSAGAGPFSATVNVTTKKTPPSQPPGNV
  VWNATDTKVLLNWEQVKAMENESEVTGYKVFYRTSSQNNVQVLNTNKTSAELVLPIKEDYIIEVKATTDG
  GDGTSSEQIRIPRITSMDARGSTSAISNVHPMSSYMPIVLFLIVYVLW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020872
• ORF Size: 3084 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020872.1, NP_065923.1
• RefSeq Size: 4997 bp
• RefSeq ORF: 3087 bp
• Locus ID: 5067
• Cytogenetics: 3p12.3
• Protein Families: Transmembrane
• Gene Summary: ''