KCNC3 (NM_004977) Human Tagged ORF Clone

CAT#: RG222238

KCNC3 (GFP-tagged) - Human potassium voltage-gated channel, Shaw-related subfamily, member 3 (KCNC3)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KCNC3
• Synonyms: KSHIIID; KV3.3; SCA13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222238 representing NM_004977
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAGCTCAGTCTGCGTCTCGTCCTTCCGCGGGCGCCAGGGGGCCAGCAAGCAGCAGCCGGCGCCAC
  CGCCGCAGCCGCCCGAGTCCCCGCCGCCGCCACCGCTGCCGCCGCAGCAGCAGCAGCCTGCGCAGCCCGG
  CCCCGCCGCGTCCCCGGCGGGCCCCCCGGCACCCCGCGGGCCCGGGGACCGGCGCGCCGAGCCATGCCCC
  GGGCTGCCGGCGGCGGCCATGGGGCGGCACGGCGGCGGCGGTGGCGACAGCGGCAAGATCGTGATCAACG
  TGGGCGGCGTGCGCCATGAGACGTACCGCTCGACGCTGCGCACCCTGCCGGGGACGCGGCTGGCCGGCCT
  GACGGAGCCCGAGGCGGCGGCACGCTTCGACTACGACCCGGGCGCCGACGAGTTCTTCTTTGACCGGCAC
  CCGGGAGTCTTCGCGTACGTGCTCAACTACTACCGCACCGGCAAGCTGCACTGCCCAGCCGACGTGTGCG
  GGCCCCTGTTTGAGGAGGAGCTCGGCTTCTGGGGCATCGACGAGACCGACGTGGAGGCCTGCTGCTGGAT
  GACCTACCGGCAGCATCGCGACGCTGAGGAGGCGCTCGACTCCTTCGAGGCGCCCGACCCCGCGGGCGCC
  GCCAACGCCGCCAACGCCGCAGGCGCCCACGACGGAGGCCTGGACGACGAGGCGGGCGCGGGCGGCGGCG
  GCCTGGACGGAGCGGGCGGCGAGCTCAAGCGCCTCTGCTTCCAGGACGCGGGCGGCGGCGCCGGGGGGCC
  GCCAGGGGGCGCGGGCGGCGCGGGCGGCACATGGTGGCGCCGCTGGCAGCCCCGCGTGTGGGCGCTCTTC
  GAGGACCCCTACTCGTCGCGGGCTGCCAGGTATGTGGCCTTCGCCTCCCTCTTCTTCATCCTCATCTCCA
  TCACCACCTTCTGCCTGGAAACCCATGAGGGCTTCATCCATATTAGCAACAAGACGGTGACCCAGGCCTC
  CCCGATCCCCGGGGCACCTCCGGAGAACATCACCAACGTGGAGGTGGAGACGGAGCCCTTCCTGACCTAC
  GTGGAGGGGGTGTGCGTGGTCTGGTTCACCTTCGAGTTCCTCATGCGCATCACCTTCTGCCCAGACAAGG
  TGGAGTTTCTTAAAAGCAGCCTCAACATCATCGACTGTGTGGCCATCCTGCCCTTCTATCTCGAGGTGGG
  CCTCTCGGGCCTCAGCTCCAAGGCCGCCAAAGACGTGCTGGGCTTCCTGCGGGTGGTCCGCTTCGTCCGC
  ATCCTGCGCATCTTCAAGCTGACCCGGCACTTCGTGGGGCTGCGCGTGCTGGGACACACGCTCCGCGCCA
  GCACCAACGAGTTCCTGCTGCTCATCATCTTCCTGGCCCTGGGGGTGCTCATCTTCGCCACCATGATTTA
  CTACGCTGAGCGCATTGGCGCCGACCCCGATGACATCCTGGGCTCCAACCACACCTACTTCAAGAACATC
  CCCATTGGCTTCTGGTGGGCTGTGGTCACCATGACGACCCTGGGCTATGGAGACATGTACCCCAAGACGT
  GGTCGGGGATGCTGGTCGGGGCGCTGTGTGCCCTGGCGGGGGTGCTGACCATCGCCATGCCTGTGCCCGT
  CATTGTCAACAACTTTGGCATGTACTATTCGCTGGCCATGGCCAAGCAGAAGCTGCCCAAGAAGAAGAAC
  AAACACATCCCCCGGCCCCCGCAACCGGGCTCGCCCAACTACTGCAAGCCTGACCCACCCCCGCCACCCC
  CGCCCCACCCGCACCACGGCAGCGGGGGCATCAGCCCGCCGCCACCCATCACCCCACCCTCCATGGGGGT
  GACTGTGGCCGGGGCCTACCCAGCGGGGCCCCACACGCACCCCGGGCTGCTCAGGGGGGGAGCGGGTGGG
  CTGGGGATCATGGGGCTGCCTCCTCTGCCAGCCCCCGGCGAGCCTTGCCCGTTGGCTCAGGAGGAGGTGA
  TTGAGATCAACCGGGCAGATCCTCGCCCCAATGGGGATCCGGCAGCAGCTGCGCTTGCCCACGAGGACTG
  CCCAGCCATTGACCAGCCTGCCATGTCCCCGGAAGACAAGAGCCCCATCACGCCTGGAAGCCGTGGCCGC
  TATAGCCGGGACCGAGCCTGCTTCCTCCTCACCGACTATGCCCCTTCCCCTGATGGCTCCATCCGAAAAG
  CCACTGGTGCTCCCCCACTGCCCCCCCAAGACTGGCGTAAGCCAGGCCCCCCAAGCTTCTTGCCCGACCT
  CAACGCCAACGCCGCGGCCTGGATATCCCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222238 representing NM_004977
  Red=Cloning site Green=Tags(s)
  
  MLSSVCVSSFRGRQGASKQQPAPPPQPPESPPPPPLPPQQQQPAQPGPAASPAGPPAPRGPGDRRAEPCP
  GLPAAAMGRHGGGGGDSGKIVINVGGVRHETYRSTLRTLPGTRLAGLTEPEAAARFDYDPGADEFFFDRH
  PGVFAYVLNYYRTGKLHCPADVCGPLFEEELGFWGIDETDVEACCWMTYRQHRDAEEALDSFEAPDPAGA
  ANAANAAGAHDGGLDDEAGAGGGGLDGAGGELKRLCFQDAGGGAGGPPGGAGGAGGTWWRRWQPRVWALF
  EDPYSSRAARYVAFASLFFILISITTFCLETHEGFIHISNKTVTQASPIPGAPPENITNVEVETEPFLTY
  VEGVCVVWFTFEFLMRITFCPDKVEFLKSSLNIIDCVAILPFYLEVGLSGLSSKAAKDVLGFLRVVRFVR
  ILRIFKLTRHFVGLRVLGHTLRASTNEFLLLIIFLALGVLIFATMIYYAERIGADPDDILGSNHTYFKNI
  PIGFWWAVVTMTTLGYGDMYPKTWSGMLVGALCALAGVLTIAMPVPVIVNNFGMYYSLAMAKQKLPKKKN
  KHIPRPPQPGSPNYCKPDPPPPPPPHPHHGSGGISPPPPITPPSMGVTVAGAYPAGPHTHPGLLRGGAGG
  LGIMGLPPLPAPGEPCPLAQEEVIEINRADPRPNGDPAAAALAHEDCPAIDQPAMSPEDKSPITPGSRGR
  YSRDRACFLLTDYAPSPDGSIRKATGAPPLPPQDWRKPGPPSFLPDLNANAAAWISP
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004977
• ORF Size: 2271 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004977.2, NP_004968.2
• RefSeq Size: 3176
• RefSeq ORF: 2274
• Locus ID: 3748
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: The Shaker gene family of Drosophila encodes components of voltage-gated potassium channels and is comprised of four subfamilies. Based on sequence similarity, this gene is similar to one of these subfamilies, namely the Shaw subfamily. The protein encoded by this gene belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. Alternate splicing results in several transcript variants. [provided by RefSeq, Mar 2014]