ADAM15 (NM_207194) Human Tagged ORF Clone

CAT#: RG222451

ADAM15 (GFP-tagged) - Human ADAM metallopeptidase domain 15 (ADAM15), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADAM15
• Synonyms: MDC15
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222451 representing NM_207194
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCTGGCGCTGCTCTGGGCCCTGGGGCTCCTGGGCGCGGGCAGCCCTCTGCCTTCCTGGCCGCTCC
  CAAATATAGGTGGCACTGAGGAGCAGCAGGCAGAGTCAGAGAAGGCCCCGAGGGAGCCCTTGGAGCCCCA
  GGTCCTTCAGGACGATCTCCCAATTAGCCTCAAAAAGGTGCTTCAGACCAGTCTGCCTGAGCCCCTGAGG
  ATCAAGTTGGAGCTGGACGGTGACAGTCATATCCTGGAGCTGCTACAGAATAGGGAGTTGGTCCCAGGCC
  GCCCAACCCTGGTGTGGTACCAGCCCGATGGCACTCGGGTGGTCAGTGAGGGACACACTTTGGAGAACTG
  CTGCTACCAGGGAAGAGTGCGGGGATATGCAGGCTCCTGGGTGTCCATCTGCACCTGCTCTGGGCTCAGA
  GGCTTGGTGGTCCTGACCCCAGAGAGAAGCTATACCCTGGAGCAGGGGCCTGGGGACCTTCAGGGTCCTC
  CCATTATTTCGCGAATCCAAGATCTCCACCTGCCAGGCCACACCTGTGCCCTGAGCTGGCGGGAATCTGT
  ACACACTCAGAAGCCACCAGAGCACCCCCTGGGACAGCGCCACATTCGCCGGAGGCGGGATGTGGTAACA
  GAGACCAAGACTGTGGAGTTGGTGATTGTGGCTGATCACTCGGAGGCCCAGAAATACCGGGACTTCCAGC
  ACCTGCTAAACCGCACACTGGAAGTGGCCCTCTTGCTGGACACATTCTTCCGGCCCCTGAATGTACGAGT
  GGCACTAGTGGGCCTGGAGGCCTGGACCCAGCGTGACCTGGTGGAGATCAGCCCAAACCCAGCTGTCACC
  CTCGAAAACTTCCTCCACTGGCGCAGGGCACATTTGCTGCCTCGATTGCCCCATGACAGTGCCCAGCTGG
  TGACTGGTACTTCATTCTCTGGGCCTACGGTGGGCATGGCCATTCAGAACTCCATCTGTTCTCCTGACTT
  CTCAGGAGGTGTGAACATGGACCACTCCACCAGCATCCTGGGAGTCGCCTCCTCCATAGCCCATGAGTTG
  GGCCACAGCCTGGGCCTGGACCATGATTTGCCTGGGAATAGCTGCCCCTGTCCAGGTCCAGCCCCAGCCA
  AGACCTGCATCATGGAGGCCTCCACAGACTTCCTACCAGGCCTGAACTTCAGCAACTGCAGCCGACGGGC
  CCTGGAGAAAGCCCTCCTGGATGGAATGGGCAGCTGCCTCTTCGAACGGCTGCCTAGCCTACCCCCTATG
  GCTGCTTTCTGCGGAAATATGTTTGTGGAGCCGGGCGAGCAGTGTGACTGTGGCTTCCTGGATGACTGCG
  TCGATCCCTGCTGTGATTCTTTGACCTGCCAGCTGAGGCCAGGTGCACAGTGTGCATCTGACGGACCCTG
  TTGTCAAAATTGCCAGCTGCGCCCGTCTGGCTGGCAGTGTCGTCCTACCAGAGGGGATTGTGACTTGCCT
  GAATTCTGCCCAGGAGACAGCTCCCAGTGTCCCCCTGATGTCAGCCTAGGGGATGGCGAGCCCTGCGCTG
  GCGGGCAAGCTGTGTGCATGCACGGGCGTTGTGCCTCCTATGCCCAGCAGTGCCAGTCACTTTGGGGACC
  TGGAGCCCAGCCCGCTGCGCCACTTTGCCTCCAGACAGCTAATACTCGGGGAAATGCTTTTGGGAGCTGT
  GGGCGCAACCCCAGTGGCAGTTATGTGTCCTGCACCCCTAGAGATGCCATTTGTGGGCAGCTCCAGTGCC
  AGACAGGTAGGACCCAGCCTCTGCTGGGCTCCATCCGGGATCTACTCTGGGAGACAATAGATGTGAATGG
  GACTGAGCTGAACTGCAGCTGGGTGCACCTGGACCTGGGCAGTGATGTGGCCCAGCCCCTCCTGACTCTG
  CCTGGCACAGCCTGTGGCCCTGGCCTGGTGTGTATAGACCATCGATGCCAGCGTGTGGATCTCCTGGGGG
  CACAGGAATGTCGAAGCAAATGCCATGGACATGGGGTCTGTGACAGCAACAGGCACTGCTACTGTGAGGA
  GGGCTGGGCACCCCCTGACTGCACCACTCAGCTCAAAGCAACCAGCTCCCTGACCACAGGGCTGCTCCTC
  AGCCTCCTGGTCTTATTGGTCCTGGTGATGCTTGGTGCCAGCTACTGGTACCGTGCCCGCCTGCACCAGC
  GACTCTGCCAGCTCAAGGGACCCACCTGCCAGTACAGGGCAGCCCAATCTGGTCCCTCTGAACGGCCAGG
  ACCTCCGCAGAGGGCCCTGCTGGCACGAGGCACTAAGCAGGCTAGTGCTCTCAGCTTCCCGGCCCCCCCT
  TCCAGGCCGCTGCCGCCTGACCCTGTGTCCAAGAGACTCCAGTCTCAGGGGCCAGCCAAGCCCCCACCCC
  CAAGGAAGCCACTGCCTGCCGACCCCCAGGGCCGGTGCCCATCGGGTGACCTGCCCGGCCCAGGGGCTGG
  AATCCCGCCCCTAGTGGTACCCTCCAGACCAGCGCCACCGCCTCCGACAGTGTCCTCGCTCTACCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222451 representing NM_207194
  Red=Cloning site Green=Tags(s)
  
  MRLALLWALGLLGAGSPLPSWPLPNIGGTEEQQAESEKAPREPLEPQVLQDDLPISLKKVLQTSLPEPLR
  IKLELDGDSHILELLQNRELVPGRPTLVWYQPDGTRVVSEGHTLENCCYQGRVRGYAGSWVSICTCSGLR
  GLVVLTPERSYTLEQGPGDLQGPPIISRIQDLHLPGHTCALSWRESVHTQKPPEHPLGQRHIRRRRDVVT
  ETKTVELVIVADHSEAQKYRDFQHLLNRTLEVALLLDTFFRPLNVRVALVGLEAWTQRDLVEISPNPAVT
  LENFLHWRRAHLLPRLPHDSAQLVTGTSFSGPTVGMAIQNSICSPDFSGGVNMDHSTSILGVASSIAHEL
  GHSLGLDHDLPGNSCPCPGPAPAKTCIMEASTDFLPGLNFSNCSRRALEKALLDGMGSCLFERLPSLPPM
  AAFCGNMFVEPGEQCDCGFLDDCVDPCCDSLTCQLRPGAQCASDGPCCQNCQLRPSGWQCRPTRGDCDLP
  EFCPGDSSQCPPDVSLGDGEPCAGGQAVCMHGRCASYAQQCQSLWGPGAQPAAPLCLQTANTRGNAFGSC
  GRNPSGSYVSCTPRDAICGQLQCQTGRTQPLLGSIRDLLWETIDVNGTELNCSWVHLDLGSDVAQPLLTL
  PGTACGPGLVCIDHRCQRVDLLGAQECRSKCHGHGVCDSNRHCYCEEGWAPPDCTTQLKATSSLTTGLLL
  SLLVLLVLVMLGASYWYRARLHQRLCQLKGPTCQYRAAQSGPSERPGPPQRALLARGTKQASALSFPAPP
  SRPLPPDPVSKRLQSQGPAKPPPPRKPLPADPQGRCPSGDLPGPGAGIPPLVVPSRPAPPPPTVSSLYL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_207194
• ORF Size: 2517 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_207194.2, NP_997077.1
• RefSeq Size: 2927
• RefSeq ORF: 2520
• Locus ID: 8751
• Protein Families: Druggable Genome, Protease, Transmembrane
• Gene Summary: The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]