PON2 (NM_000305) Human Tagged ORF Clone
CAT#: RG222624
- TrueORF®
PON2 (GFP-tagged) - Human paraoxonase 2 (PON2), transcript variant 1
"NM_000305" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Symbol | PON2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG222624 representing NM_000305
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGGCTGGTGGCTGTGGGCTTGCTGGGGATCGCGCTGGCGCTCCTGGGCGAGAGGCTTCTGGCAC TCAGAAATCGACTTAAAGCCTCCAGAGAAGTAGAATCTGTAGACCTTCCACACTGCCACCTGATTAAAGG AATTGAAGCTGGCTCTGAAGATATTGACATACTTCCCAATGGTCTGGCTTTTTTTAGTGTGGGTCTAAAA TTCCCAGGACTCCACAGCTTTGCACCAGATAAGCCTGGAGGAATACTAATGATGGATCTAAAAGAAGAAA AACCAAGGGCACGGGAATTAAGAATCAGTCGTGGGTTTGATTTGGCCTCATTCAATCCACATGGCATCAG CACTTTCATAGACAACGATGACACAGTTTATCTCTTTGTTGTAAACCACCCAGAATTCAAGAATACAGTG GAAATTTTTAAATTTGAAGAAGCAGAAAATTCTCTGTTGCATCTGAAAACAGTCAAACATGAGCTTCTTC CAAGTGTGAATGACATCACAGCTGTTGGACCGGCACATTTCTATGCCACAAATGACCACTACTTCTCTGA TCCTTTCTTAAAGTATTTAGAAACATACTTGAACTTACACTGGGCAAATGTTGTTTACTACAGTCCAAAT GAAGTTAAAGTGGTAGCAGAAGGATTTGATTCAGCAAATGGGATCAATATTTCACCTGATGATAAGTATA TCTATGTTGCTGACATATTGGCTCATGAAATTCATGTTTTGGAAAAACACACTAATATGAATTTAACTCA GTTGAAGGTACTTGAGCTGGATACACTGGTGGATAATTTATCTATTGATCCTTCCTCGGGGGACATCTGG GTAGGCTGTCATCCTAATGGCCAGAAGCTCTTCGTGTATGACCCGAACAATCCTCCCTCGTCAGAGGTTC TCCGCATCCAGAACATTCTATCTGAGAAGCCTACAGTGACTACAGTTTATGCCAACAATGGGTCTGTTCT CCAAGGAAGTTCTGTAGCCTCAGTGTATGATGGGAAGCTGCTCATAGGCACTTTATACCACAGAGCCTTG TATTGTGAACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG222624 representing NM_000305
Red=Cloning site Green=Tags(s) MGRLVAVGLLGIALALLGERLLALRNRLKASREVESVDLPHCHLIKGIEAGSEDIDILPNGLAFFSVGLK FPGLHSFAPDKPGGILMMDLKEEKPRARELRISRGFDLASFNPHGISTFIDNDDTVYLFVVNHPEFKNTV EIFKFEEAENSLLHLKTVKHELLPSVNDITAVGPAHFYATNDHYFSDPFLKYLETYLNLHWANVVYYSPN EVKVVAEGFDSANGINISPDDKYIYVADILAHEIHVLEKHTNMNLTQLKVLELDTLVDNLSIDPSSGDIW VGCHPNGQKLFVYDPNNPPSSEVLRIQNILSEKPTVTTVYANNGSVLQGSSVASVYDGKLLIGTLYHRAL YCEL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000305 |
ORF Size | 1062 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000305.2, NP_000296.2 |
RefSeq Size | 1669 bp |
RefSeq ORF | 1065 bp |
Locus ID | 5445 |
Cytogenetics | 7q21.3 |
Domains | Arylesterase |
Protein Families | Druggable Genome |
Protein Pathways | Metabolic pathways |
Gene Summary | 'This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC119958 | PON2 (untagged)-Human paraoxonase 2 (PON2), transcript variant 1 |
USD 310.00 |
|
RC222624 | PON2 (Myc-DDK-tagged)-Human paraoxonase 2 (PON2), transcript variant 1 |
USD 420.00 |
|
RC222624L1 | Lenti ORF clone of Human paraoxonase 2 (PON2), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC222624L2 | Lenti ORF clone of Human paraoxonase 2 (PON2), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC222624L3 | Lenti ORF clone of Human paraoxonase 2 (PON2), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC222624L4 | Lenti ORF clone of Human paraoxonase 2 (PON2), transcript variant 1, mGFP tagged |
USD 620.00 |
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