PON2 (NM_000305) Human Untagged Clone

CAT#: SC119958

PON2 (untagged)-Human paraoxonase 2 (PON2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PON2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119958 sequence for NM_000305 edited (data generated by NextGen Sequencing)
  ATGGGGCGGCTGGTGGCTGTGGGCTTGCTGGGGATCGCGCTGGCGCTCCTGGGCGAGAGG
  CTTCTGGCACTCAGAAATCGACTTAAAGCCTCCAGAGAAGTAGAATCTGTAGACCTTCCA
  CACTGCCACCTGATTAAAGGAATTGAAGCTGGCTCTGAAGATATTGACATACTTCCCAAT
  GGTCTGGCTTTTTTTAGTGTGGGTCTAAAATTCCCAGGACTCCACAGCTTTGCACCAGAT
  AAGCCTGGAGGAATACTAATGATGGATCTAAAAGAAGAAAAACCAAGGGCACGGGAATTA
  AGAATCAGTCGTGGGTTTGATTTGGCCTCATTCAATCCACATGGCATCAGCACTTTCATA
  GACAACGATGACACAGTTTATCTCTTTGTTGTAAACCACCCAGAATTCAAGAATACAGTG
  GAAATTTTTAAATTTGAAGAAGCAGAAAATTCTCTGTTGCATCTGAAAACAGTCAAACAT
  GAGCTTCTTCCAAGTGTGAATGACATCACAGCTGTTGGACCGGCACATTTCTATGCCACA
  AATGACCACTACTTCTCTGATCCTTTCTTAAAGTATTTAGAAACATACTTGAACTTACAC
  TGGGCAAATGTTGTTTACTACAGTCCAAATGAAGTTAAAGTGGTAGCAGAAGGATTTGAT
  TCAGCAAATGGGATCAATATTTCACCTGATGATAAGTATATCTATGTTGCTGACATATTG
  GCTCATGAAATTCATGTTTTGGAAAAACACACTAATATGAATTTAACTCAGTTGAAGGTA
  CTTGAGCTGGATACACTGGTGGATAATTTATCTATTGATCCTTCCTCGGGGGACATCTGG
  GTAGGCTGTCATCCTAATGGCCAGAAGCTCTTCGTGTATGACCCGAACAATCCTCCCTCG
  TCAGAGGTTCTCCGCATCCAGAACATTCTATCTGAGAAGCCTACAGTGACTACAGTTTAT
  GCCAACAATGGGTCTGTTCTCCAAGGAAGTTCTGTAGCCTCAGTGTATGATGGGAAGCTG
  CTCATAGGCACTTTATACCACAGAGCCTTGTATTGTGAACTCTAA
  
  Clone variation with respect to NM_000305.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000305 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCCCACGGAGCTGCT
  GGCCAGGCCGGAGCGAGGCAGCGCGCCCGGCTCCCGCGCCATGGGGCGGCTGGTGGCTGT
  GGGCTTGCTGGGGATCGCGCTGGCGCTCCTGGGCGAGAGGCTTCTGGCACTCAGAAATCG
  ACTTAAAGCCTCCAGAGAAGTAGAATCTGTAGACCTTCCACACTGCCACCTGATTAAAGG
  AATTGAAGCTGGCTCTGAAGATATTGACATACTTCCCAATGGTCTGGCTTTTTTTAGTGT
  GGGTCTAAAATTCCCAGGACTCCACAGCTTTGCACCAGATAAGCCTGGAGGAATACTAAT
  GATGGATCTAAAAGAAGAAAAACCAAGGGCACGGGAATTAAGAATCAGTCGTGGGTTTGA
  TTTGGCCTCATTCAATCCACATGGCATCAGCACTTTCATAGACAACGATGACACAGTTTA
  TCTCTTTGTTGTAAACCACCCAGAATTCAAGAATACAGTGGAAATTTTTAAATTTGAAGA
  AGCAGAAAATTCTCTGTTGCATCTGAAAACAGTCAAACATGAGCTTCTTCCAAGTGTGAA
  TGACATCACAGCTGTTGGACCGGCACATTTCTATGCCACAAATGACCACTACTTCTCTGA
  TCCTTTCTTANAGTATTTAGAAACATACTTGAACTTACACTGGGCANATGTTGTTTACTA
  CAGTCCANATGAAGTTAAAGTGGTAGCAGAAGGATTTGATTCAGCANATGGGATCAATAT
  TTCACCTGGATGATAGTATATCTATGGTGCTGACATATAGGCTCATGAAATNCATGGTNT
  GGAAAAACACACTNATATGAANTTAACTCAGTTGAAAGGTACTTGAGCTGGATACACTGG
  NTGGATATTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000305 unedited
  NNCCCCAAGCTCTGNATCCGCGGCCGCAATCTAGCATCGAGTTTTTTTTTTTTTTTTTTG
  GGATTTTACCTACTCTTTAATATAACTCCAACTTTTTAATGGTAAACAAAGATGTAAGTA
  CAAAACATCAAAATACCCTATCAGTAGTTCTAAACAGCCATAGTAGTCACAGTGCCAGAA
  GTGAGGTCACTCACATTTTAAGGAAATATAATTCACTCTATTTCAGTGGAATCCATGTTC
  TGGCAGTTGGAAGGCAAAGGTGAGGCTTACTTTGCGCACAATGCATTCACTTTATTCCAA
  AGCAGATTTCTTCTCTGTCCCTTGCTTGGCATTTCAAACAACCTGCTCATCTCTCTTTTT
  TGTTAAAAGCGCTCTAAGAACTAAAAGGGCCGTTCCTTACTGGAATAAAATTAACTACAC
  ATGCCATACATTTCTGGGTCAATGTTGCTGGCCAACATTCCCTCAGAACTCACAATCCAT
  CGAACATTAATTGCTAAGTTATTGCACTTTTCTGCCAAAAGCACCATTCACAGTTCACCA
  TACAAGGCTCTGCGCTATAACAGCGCCTATGAGCAGCCTCCCATCATACACTGAGGCTAC
  AGAACTTCCTTGGGAACAGACTCACTGCCGCACACACTGCACTACTGCCCGCTTCCACAC
  AGAAGTTCCGGACGCCGAGAACCTCTGACTCGGGAGGATTGCTTCGGGTCATACACCAAG
  AGCCTTTGGCCCTTAAGACGACAGCCTACCCATATTGTCCCCCGAGGAAGGATCAATAGA
  TAACATATCCACCATGGTTTCCCGCCAACATACCTTTACTCGCACTAACCCATAACCAGG
  GCGGCCTTCCCACACCATGAACTTCATGTACCACCTCCCGACCCCCAATGTTATTCCTCC
  CAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000305
• Insert Size: 2450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000305.2, NP_000296.2
• RefSeq Size: 1669 bp
• RefSeq ORF: 1065 bp
• Locus ID: 5445
• Cytogenetics: 7q21.3
• Domains: Arylesterase
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways
• Gene Summary: 'This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).