PON2 (NM_000305) Human Untagged Clone

CAT#: SC119958

PON2 (untagged)-Human paraoxonase 2 (PON2), transcript variant 1


  "NM_000305" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PON2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119958 sequence for NM_000305 edited (data generated by NextGen Sequencing)
ATGGGGCGGCTGGTGGCTGTGGGCTTGCTGGGGATCGCGCTGGCGCTCCTGGGCGAGAGG
CTTCTGGCACTCAGAAATCGACTTAAAGCCTCCAGAGAAGTAGAATCTGTAGACCTTCCA
CACTGCCACCTGATTAAAGGAATTGAAGCTGGCTCTGAAGATATTGACATACTTCCCAAT
GGTCTGGCTTTTTTTAGTGTGGGTCTAAAATTCCCAGGACTCCACAGCTTTGCACCAGAT
AAGCCTGGAGGAATACTAATGATGGATCTAAAAGAAGAAAAACCAAGGGCACGGGAATTA
AGAATCAGTCGTGGGTTTGATTTGGCCTCATTCAATCCACATGGCATCAGCACTTTCATA
GACAACGATGACACAGTTTATCTCTTTGTTGTAAACCACCCAGAATTCAAGAATACAGTG
GAAATTTTTAAATTTGAAGAAGCAGAAAATTCTCTGTTGCATCTGAAAACAGTCAAACAT
GAGCTTCTTCCAAGTGTGAATGACATCACAGCTGTTGGACCGGCACATTTCTATGCCACA
AATGACCACTACTTCTCTGATCCTTTCTTAAAGTATTTAGAAACATACTTGAACTTACAC
TGGGCAAATGTTGTTTACTACAGTCCAAATGAAGTTAAAGTGGTAGCAGAAGGATTTGAT
TCAGCAAATGGGATCAATATTTCACCTGATGATAAGTATATCTATGTTGCTGACATATTG
GCTCATGAAATTCATGTTTTGGAAAAACACACTAATATGAATTTAACTCAGTTGAAGGTA
CTTGAGCTGGATACACTGGTGGATAATTTATCTATTGATCCTTCCTCGGGGGACATCTGG
GTAGGCTGTCATCCTAATGGCCAGAAGCTCTTCGTGTATGACCCGAACAATCCTCCCTCG
TCAGAGGTTCTCCGCATCCAGAACATTCTATCTGAGAAGCCTACAGTGACTACAGTTTAT
GCCAACAATGGGTCTGTTCTCCAAGGAAGTTCTGTAGCCTCAGTGTATGATGGGAAGCTG
CTCATAGGCACTTTATACCACAGAGCCTTGTATTGTGAACTCTAA

Clone variation with respect to NM_000305.2
>OriGene 5' read for NM_000305 unedited
GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGCCCACGGAGCTGCT
GGCCAGGCCGGAGCGAGGCAGCGCGCCCGGCTCCCGCGCCATGGGGCGGCTGGTGGCTGT
GGGCTTGCTGGGGATCGCGCTGGCGCTCCTGGGCGAGAGGCTTCTGGCACTCAGAAATCG
ACTTAAAGCCTCCAGAGAAGTAGAATCTGTAGACCTTCCACACTGCCACCTGATTAAAGG
AATTGAAGCTGGCTCTGAAGATATTGACATACTTCCCAATGGTCTGGCTTTTTTTAGTGT
GGGTCTAAAATTCCCAGGACTCCACAGCTTTGCACCAGATAAGCCTGGAGGAATACTAAT
GATGGATCTAAAAGAAGAAAAACCAAGGGCACGGGAATTAAGAATCAGTCGTGGGTTTGA
TTTGGCCTCATTCAATCCACATGGCATCAGCACTTTCATAGACAACGATGACACAGTTTA
TCTCTTTGTTGTAAACCACCCAGAATTCAAGAATACAGTGGAAATTTTTAAATTTGAAGA
AGCAGAAAATTCTCTGTTGCATCTGAAAACAGTCAAACATGAGCTTCTTCCAAGTGTGAA
TGACATCACAGCTGTTGGACCGGCACATTTCTATGCCACAAATGACCACTACTTCTCTGA
TCCTTTCTTANAGTATTTAGAAACATACTTGAACTTACACTGGGCANATGTTGTTTACTA
CAGTCCANATGAAGTTAAAGTGGTAGCAGAAGGATTTGATTCAGCANATGGGATCAATAT
TTCACCTGGATGATAGTATATCTATGGTGCTGACATATAGGCTCATGAAATNCATGGTNT
GGAAAAACACACTNATATGAANTTAACTCAGTTGAAAGGTACTTGAGCTGGATACACTGG
NTGGATATTT
>OriGene 3' read for NM_000305 unedited
NNCCCCAAGCTCTGNATCCGCGGCCGCAATCTAGCATCGAGTTTTTTTTTTTTTTTTTTG
GGATTTTACCTACTCTTTAATATAACTCCAACTTTTTAATGGTAAACAAAGATGTAAGTA
CAAAACATCAAAATACCCTATCAGTAGTTCTAAACAGCCATAGTAGTCACAGTGCCAGAA
GTGAGGTCACTCACATTTTAAGGAAATATAATTCACTCTATTTCAGTGGAATCCATGTTC
TGGCAGTTGGAAGGCAAAGGTGAGGCTTACTTTGCGCACAATGCATTCACTTTATTCCAA
AGCAGATTTCTTCTCTGTCCCTTGCTTGGCATTTCAAACAACCTGCTCATCTCTCTTTTT
TGTTAAAAGCGCTCTAAGAACTAAAAGGGCCGTTCCTTACTGGAATAAAATTAACTACAC
ATGCCATACATTTCTGGGTCAATGTTGCTGGCCAACATTCCCTCAGAACTCACAATCCAT
CGAACATTAATTGCTAAGTTATTGCACTTTTCTGCCAAAAGCACCATTCACAGTTCACCA
TACAAGGCTCTGCGCTATAACAGCGCCTATGAGCAGCCTCCCATCATACACTGAGGCTAC
AGAACTTCCTTGGGAACAGACTCACTGCCGCACACACTGCACTACTGCCCGCTTCCACAC
AGAAGTTCCGGACGCCGAGAACCTCTGACTCGGGAGGATTGCTTCGGGTCATACACCAAG
AGCCTTTGGCCCTTAAGACGACAGCCTACCCATATTGTCCCCCGAGGAAGGATCAATAGA
TAACATATCCACCATGGTTTCCCGCCAACATACCTTTACTCGCACTAACCCATAACCAGG
GCGGCCTTCCCACACCATGAACTTCATGTACCACCTCCCGACCCCCAATGTTATTCCTCC
CAT
Restriction Sites NotI-NotI     
ACCN NM_000305
Insert Size 2450 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000305.2, NP_000296.2
RefSeq Size 1669 bp
RefSeq ORF 1065 bp
Locus ID 5445
Cytogenetics 7q21.3
Domains Arylesterase
Protein Families Druggable Genome
Protein Pathways Metabolic pathways
Gene Summary 'This gene encodes a member of the paraoxonase gene family, which includes three known members located adjacent to each other on the long arm of chromosome 7. The encoded protein is ubiquitously expressed in human tissues, membrane-bound, and may act as a cellular antioxidant, protecting cells from oxidative stress. Hydrolytic activity against acylhomoserine lactones, important bacterial quorum-sensing mediators, suggests the encoded protein may also play a role in defense responses to pathogenic bacteria. Mutations in this gene may be associated with vascular disease and a number of quantitative phenotypes related to diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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