PKMYT1 (NM_004203) Human Tagged ORF Clone

CAT#: RG222657

PKMYT1 (GFP-tagged) - Human protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PKMYT1
• Synonyms: MYT1; PPP1R126
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222657 representing NM_004203
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTAGAACGGCCTCCTGCACTGGCCATGCCCATGCCCACGGAGGGCACCCCGCCACCTCTGAGTGGCA
  CCCCCATCCCAGTCCCAGCCTACTTCCGCCACGCAGAACCTGGATTCTCCCTCAAGAGGCCCAGGGGGCT
  CAGCCGGAGCCTCCCACCTCCGCCCCCTGCCAAGGGCAGCATTCCCATCAGCCGCCTCTTCCCTCCTCGG
  ACCCCAGGCTGGCACCAGCTGCAGCCCCGGCGGGTGTCATTCCGGGGCGAGGCCTCAGAGACTCTGCAGA
  GCCCTGGGTATGACCCAAGCCGGCCAGAGTCCTTCTTCCAGCAGAGCTTCCAGAGGCTCAGCCGCCTGGG
  CCATGGCTCCTACGGAGAGGTCTTCAAGGTGCGCTCCAAGGAGGACGGCCGGCTCTATGCGGTAAAGCGT
  TCCATGTCACCATTCCGGGGCCCCAAGGACCGGGCCCGCAAGTTGGCCGAGGTGGGCAGCCACGAGAAGG
  TGGGGCAGCACCCATGCTGCGTGCGGCTGGAGCAGGCCTGGGAGGAGGGCGGCATCCTGTACCTGCAGAC
  GGAGCTGTGCGGGCCCAGCCTGCAGCAACACTGTGAGGCCTGGGGTGCCAGCCTGCCTGAGGCCCAGGTC
  TGGGGCTACCTGCGGGACACGCTGCTTGCCCTGGCCCATCTGCACAGCCAGGGCCTGGTGCACCTTGATG
  TCAAGCCTGCCAACATCTTCCTGGGGCCCCGGGGCCGCTGCAAGCTGGGTGACTTCGGACTGCTGGTGGA
  GCTGGGTACAGCAGGAGCTGGTGAGGTCCAGGAGGGAGACCCCCGCTACATGGCCCCCGAGCTGCTGCAG
  GGCTCCTATGGGACAGCAGCGGATGTGTTCAGTCTGGGCCTCACCATCCTGGAAGTGGCATGCAACATGG
  AGCTGCCCCACGGTGGGGAGGGCTGGCAGCAGCTGCGCCAGGGCTACCTGCCCCCTGAGTTCACTGCCGG
  TCTGTCTTCCGAGCTGCGTTCTGTCCTTGTCATGATGCTGGAGCCAGACCCCAAGCTGCGGGCCACGGCC
  GAGGCCCTGCTGGCACTGCCTGTGTTGAGGCAGCCGCGGGCCTGGGGTGTGCTGTGGTGCATGGCAGCGG
  AGGCCCTGAGCCGAGGGTGGGCCCTGTGGCAGGCCCTGCTTGCCCTGCTCTGCTGGCTCTGGCATGGGCT
  GGCTCACCCTGCCAGCTGGCTACAGCCCCTGGGCCCGCCAGCCACCCCGCCTGGCTCACCACCCTGCAGT
  TTGCTCCTGGACAGCAGCCTCTCCAGCAACTGGGATGACGACAGCCTAGGGCCTTCACTCTCCCCTGAGG
  CTGTCCTGGCCCGGACTGTGGGGAGCACCTCCACCCCCCGGAGCAGGTGCACACCCAGGGATGCCCTGGA
  CCTAAGTGACATCAACTCAGAGCCTCCTCGGGGCTCCTTCCCCTCCTTTGAGCCTCGGAACCTCCTCAGC
  CTGTTTGAGGACACCCTAGACCCAACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222657 representing NM_004203
  Red=Cloning site Green=Tags(s)
  
  MLERPPALAMPMPTEGTPPPLSGTPIPVPAYFRHAEPGFSLKRPRGLSRSLPPPPPAKGSIPISRLFPPR
  TPGWHQLQPRRVSFRGEASETLQSPGYDPSRPESFFQQSFQRLSRLGHGSYGEVFKVRSKEDGRLYAVKR
  SMSPFRGPKDRARKLAEVGSHEKVGQHPCCVRLEQAWEEGGILYLQTELCGPSLQQHCEAWGASLPEAQV
  WGYLRDTLLALAHLHSQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGTAGAGEVQEGDPRYMAPELLQ
  GSYGTAADVFSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRSVLVMMLEPDPKLRATA
  EALLALPVLRQPRAWGVLWCMAAEALSRGWALWQALLALLCWLWHGLAHPASWLQPLGPPATPPGSPPCS
  LLLDSSLSSNWDDDSLGPSLSPEAVLARTVGSTSTPRSRCTPRDALDLSDINSEPPRGSFPSFEPRNLLS
  LFEDTLDPT
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004203
• ORF Size: 1497 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004203.3, NP_004194.3
• RefSeq Size: 2121
• RefSeq ORF: 1500
• Locus ID: 9088
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]