PKMYT1 (NM_004203) Human Untagged Clone

CAT#: SC323456

PKMYT1 (untagged)-Kinase deficient mutant (K139M) of Human protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1


  "NM_004203" in other vectors (7)

Reconstitution Protocol

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    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PKMYT1
Synonyms MYT1; PPP1R126
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC323456 sequence for NM_004203 edited (data generated by NextGen Sequencing)
ATGCTAGAACGGCCTCCTGCACTGGCCATGCCCATGCCCACGGAGGGCACCCCGCCACCT
CTGAGTGGCACCCCCATCCCAGTCCCAGCCTACTTCCGCCACGCAGAACCTGGATTCTCC
CTCAAGAGGCCCAGGGGGCTCAGCCGGAGCCTCCCACCTCCGCCCCCTGCCAAGGGCAGC
ATTCCCATCAGCCGCCTCTTCCCTCCTCGGACCCCAGGCTGGCACCAGCTGCAGCCCCGG
CGGGTGTCATTCCGGGGCGAGGCCTCAGAGACTCTGCAGAGCCCTGGGTATGACCCAAGC
CGGCCAGAGTCCTTCTTCCAGCAGAGCTTCCAGAGGCTCAGCCGCCTGGGCCATGGCTCC
TACGGAGAGGTCTTCAAGGTGCGCTCCAAGGAGGACGGCCGGCTCTATGCGGTAATGCGT
TCCATGTCACCATTCCGGGGCCCCAAGGACCGGGCCCGCAAGTTGGCCGAGGTGGGCAGC
CACGAGAAGGTGGGGCAGCACCCATGCTGCGTGCGGCTGGAGCAGGCCTGGGAGGAGGGC
GGCATCCTGTACCTGCAGACGGAGCTGTGCGGGCCCAGCCTGCAGCAACACTGTGAGGCC
TGGGGTGCCAGCCTGCCTGAGGCCCAGGTCTGGGGCTACCTGCGGGACACGCTGCTTGCC
CTGGCCCATCTGCACAGCCAGGGCCTGGTGCACCTTGATGTCAAGCCTGCCAACATCTTC
CTGGGGCCCCGGGGCCGCTGCAAGCTGGGTGACTTCGGACTGCTGGTGGAGCTGGGTACA
GCAGGAGCTGGTGAGGTCCAGGAGGGAGACCCCCGCTACATGGCCCCCGAGCTGCTGCAG
GGCTCCTATGGGACAGCAGCGGATGTGTTCAGTCTGGGCCTCACCATCCTGGAAGTGGCA
TGCAACATGGAGCTGCCCCACGGTGGGGAGGGCTGGCAGCAGCTGCGCCAGGGCTACCTG
CCCCCTGAGTTCACTGCCGGTCTGTCTTCCGAGCTGCGTTCTGTCCTTGTCATGATGCTG
GAGCCAGACCCCAAGCTGCGGGCCACGGCCGAGGCCCTGCTGGCACTGCCTGTGTTGAGG
CAGCCGCGGGCCTGGGGTGTGCTGTGGTGCATGGCAGCGGAGGCCCTGAGCCGAGGGTGG
GCCCTGTGGCAGGCCCTGCTTGCCCTGCTCTGCTGGCTCTGGCATGGGCTGGCTCACCCT
GCCAGCTGGCTACAGCCCCTGGGCCCGCCAGCCACCCCGCCTGGCTCACCACCCTGCAGT
TTGCTCCTGGACAGCAGCCTCTCCAGCAACTGGGATGACGACAGCCTAGGGCCTTCACTC
TCCCCTGAGGCTGTCCTGGCCCGGACTGTGGGGAGCACCTCCACCCCCCGGAGCAGGTGC
ACACCCAGGGATGCCCTGGACCTAAGTGACATCAACTCAGAGCCTCCTCGGGGCTCCTTC
CCCTCCTTTGAGCCTCGGAACCTCCTCAGCCTGTTTGAGGACACCCTAGACCCAACCTGA

Clone variation with respect to NM_004203.4
416 a=>t
>OriGene 5' read for mutant NM_004203 unedited
ACCGCCCGTATCAGCAACTGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAG
TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCGCGCGTT
CACGGGCCGTTCCCCCTCACGGGAGTCCTCCGCCCGGGCGTCCGGAACAGTCGACGGCAGACTCCGGCCC
GCTGAGCCACCCGAGGGGTCCCGTGGCCTCCGCGGACCCGGAATCTGGGCCCTCGCGGACCCGCGCCCCG
CCCAGTCGCCCCAGGGCTTCCCCACACCCACGGAGTGAAGTCAGCCGCGGCCCTGCCTGGGAGGGAACTT
ACCGTCTACCGGGAAAAGGTGGGCCAGCAGATGTGTCGGGCCTGGTGAAGAGGGTGAAGGCGAAGACGGC
CCGATCGCCAAGGGCCCCGGAAGCTGCGGAGGTCACCCCGCCCTGCCCTAAGCTCAGGGACACCCTGAAT
TCCTTGGAACCCCTGGTTCTTGCCTCCCCGTTCCCACCATTGAGGCTGTTGGGCCAGGCCAGTTATGCTA
AACGGCCTCTGCTGGGCCATGCCATCCCCGAGGGGCCCCGCCCCCCTTAAGGGCACAATACCATTGCAAC
TATTTTGCGCACCAAACGGGTTTCACCAAAGCCCAGGGCGTCC
>SC323456 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
GSGRTCTTCTCAGCAGAGCTTCCAGAGGCTCAGCCGCCTGGGCCATGGCTCCTACGGAGAGGTCTTCAAG
GTGCGCTCCAAGGAGGACGGCCGGCTCTATGCGGTAATGCGTTCCATGTCACCATTCCGGGGCCCCAAGG
ACCGGGCCCGCAAGTTGGCCGAGGTGGGCAGCCACGAGAAGGTGGGGCAGCACCCATGCTGCGTGCGGCT
GGAGCAGGCCTGGGAGGAGGGCGGCATCCTGTACCTGCAGACGGA
Restriction Sites Please inquire     
ACCN NM_004203
ORF Size 1500 bp
Insert Size 2190
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
Reference Data
RefSeq NM_004203.3, NP_004194.3
RefSeq Size 2121
RefSeq ORF 1500
Locus ID 9088
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
Gene Summary This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
Transcript Variant: This variant (1) encodes the longest isoform (1).

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