PKMYT1 (NM_004203) Human Untagged Clone

CAT#: SC323456

PKMYT1 (untagged)-Kinase deficient mutant (K139M) of Human protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PKMYT1
• Synonyms: MYT1; PPP1R126
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323456 sequence for NM_004203 edited (data generated by NextGen Sequencing)
  ATGCTAGAACGGCCTCCTGCACTGGCCATGCCCATGCCCACGGAGGGCACCCCGCCACCT
  CTGAGTGGCACCCCCATCCCAGTCCCAGCCTACTTCCGCCACGCAGAACCTGGATTCTCC
  CTCAAGAGGCCCAGGGGGCTCAGCCGGAGCCTCCCACCTCCGCCCCCTGCCAAGGGCAGC
  ATTCCCATCAGCCGCCTCTTCCCTCCTCGGACCCCAGGCTGGCACCAGCTGCAGCCCCGG
  CGGGTGTCATTCCGGGGCGAGGCCTCAGAGACTCTGCAGAGCCCTGGGTATGACCCAAGC
  CGGCCAGAGTCCTTCTTCCAGCAGAGCTTCCAGAGGCTCAGCCGCCTGGGCCATGGCTCC
  TACGGAGAGGTCTTCAAGGTGCGCTCCAAGGAGGACGGCCGGCTCTATGCGGTAATGCGT
  TCCATGTCACCATTCCGGGGCCCCAAGGACCGGGCCCGCAAGTTGGCCGAGGTGGGCAGC
  CACGAGAAGGTGGGGCAGCACCCATGCTGCGTGCGGCTGGAGCAGGCCTGGGAGGAGGGC
  GGCATCCTGTACCTGCAGACGGAGCTGTGCGGGCCCAGCCTGCAGCAACACTGTGAGGCC
  TGGGGTGCCAGCCTGCCTGAGGCCCAGGTCTGGGGCTACCTGCGGGACACGCTGCTTGCC
  CTGGCCCATCTGCACAGCCAGGGCCTGGTGCACCTTGATGTCAAGCCTGCCAACATCTTC
  CTGGGGCCCCGGGGCCGCTGCAAGCTGGGTGACTTCGGACTGCTGGTGGAGCTGGGTACA
  GCAGGAGCTGGTGAGGTCCAGGAGGGAGACCCCCGCTACATGGCCCCCGAGCTGCTGCAG
  GGCTCCTATGGGACAGCAGCGGATGTGTTCAGTCTGGGCCTCACCATCCTGGAAGTGGCA
  TGCAACATGGAGCTGCCCCACGGTGGGGAGGGCTGGCAGCAGCTGCGCCAGGGCTACCTG
  CCCCCTGAGTTCACTGCCGGTCTGTCTTCCGAGCTGCGTTCTGTCCTTGTCATGATGCTG
  GAGCCAGACCCCAAGCTGCGGGCCACGGCCGAGGCCCTGCTGGCACTGCCTGTGTTGAGG
  CAGCCGCGGGCCTGGGGTGTGCTGTGGTGCATGGCAGCGGAGGCCCTGAGCCGAGGGTGG
  GCCCTGTGGCAGGCCCTGCTTGCCCTGCTCTGCTGGCTCTGGCATGGGCTGGCTCACCCT
  GCCAGCTGGCTACAGCCCCTGGGCCCGCCAGCCACCCCGCCTGGCTCACCACCCTGCAGT
  TTGCTCCTGGACAGCAGCCTCTCCAGCAACTGGGATGACGACAGCCTAGGGCCTTCACTC
  TCCCCTGAGGCTGTCCTGGCCCGGACTGTGGGGAGCACCTCCACCCCCCGGAGCAGGTGC
  ACACCCAGGGATGCCCTGGACCTAAGTGACATCAACTCAGAGCCTCCTCGGGGCTCCTTC
  CCCTCCTTTGAGCCTCGGAACCTCCTCAGCCTGTTTGAGGACACCCTAGACCCAACCTGA
  
  Clone variation with respect to NM_004203.4
  416 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004203 unedited
  ACCGCCCGTATCAGCAACTGGGCGGTAGGCGCTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAG
  TGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCGCGCGTT
  CACGGGCCGTTCCCCCTCACGGGAGTCCTCCGCCCGGGCGTCCGGAACAGTCGACGGCAGACTCCGGCCC
  GCTGAGCCACCCGAGGGGTCCCGTGGCCTCCGCGGACCCGGAATCTGGGCCCTCGCGGACCCGCGCCCCG
  CCCAGTCGCCCCAGGGCTTCCCCACACCCACGGAGTGAAGTCAGCCGCGGCCCTGCCTGGGAGGGAACTT
  ACCGTCTACCGGGAAAAGGTGGGCCAGCAGATGTGTCGGGCCTGGTGAAGAGGGTGAAGGCGAAGACGGC
  CCGATCGCCAAGGGCCCCGGAAGCTGCGGAGGTCACCCCGCCCTGCCCTAAGCTCAGGGACACCCTGAAT
  TCCTTGGAACCCCTGGTTCTTGCCTCCCCGTTCCCACCATTGAGGCTGTTGGGCCAGGCCAGTTATGCTA
  AACGGCCTCTGCTGGGCCATGCCATCCCCGAGGGGCCCCGCCCCCCTTAAGGGCACAATACCATTGCAAC
  TATTTTGCGCACCAAACGGGTTTCACCAAAGCCCAGGGCGTCC
  
  Kinase domain sequence

  >SC323456 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  GSGRTCTTCTCAGCAGAGCTTCCAGAGGCTCAGCCGCCTGGGCCATGGCTCCTACGGAGAGGTCTTCAAG
  GTGCGCTCCAAGGAGGACGGCCGGCTCTATGCGGTAATGCGTTCCATGTCACCATTCCGGGGCCCCAAGG
  ACCGGGCCCGCAAGTTGGCCGAGGTGGGCAGCCACGAGAAGGTGGGGCAGCACCCATGCTGCGTGCGGCT
  GGAGCAGGCCTGGGAGGAGGGCGGCATCCTGTACCTGCAGACGGA
  
• Restriction Sites: Please inquire
• ACCN: NM_004203
• ORF Size: 1500 bp
• Insert Size: 2190
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_004203.3, NP_004194.3
• RefSeq Size: 2121
• RefSeq ORF: 1500
• Locus ID: 9088
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
  Transcript Variant: This variant (1) encodes the longest isoform (1).