ZNF148 (NM_021964) Human Tagged ORF Clone

CAT#: RG222687

ZNF148 (GFP-tagged) - Human zinc finger protein 148 (ZNF148)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ZNF148
• Synonyms: BERF-1; BFCOL1; GDACCF; HT-BETA; pHZ-52; ZBP-89; ZFP148
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222687 representing NM_021964
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACATTGACGACAAACTGGAAGGATTGTTTCTTAAATGTGGCGGCATAGACGAAATGCAGTCTTCCA
  GGACAATGGTTGTAATGGGTGGAGTGTCTGGCCAGTCTACTGTGTCTGGAGAGCTACAGGATTCAGTACT
  TCAAGATCGAAGTATGCCTCACCAGGAGATCCTTGCTGCAGATGAAGTGTTACAAGAAAGTGAAATGAGA
  CAACAGGATATGATATCACATGATGAACTCATGGTCCATGAGGAGACAGTGAAAAATGATGAAGAGCAGA
  TGGAAACACATGAAAGACTTCCTCAAGGACTACAGTATGCACTTAATGTCCCTATAAGCGTAAAGCAGGA
  AATTACTTTTACTGATGTATCTGAGCAACTGATGAGAGACAAAAAACAAATCAGAGAGCCAGTAGACTTA
  CAGAAAAAGAAGAAGCGGAAACAACGTTCTCCCGCAAAAATCCTTACAATAAATGAGGATGGATCACTTG
  GTTTGAAAACCCCTAAATCTCACGTTTGTGAGCACTGCAATGCTGCCTTTAGAACGAACTATCACTTACA
  GAGACATGTCTTCATTCATACAGGTGAAAAACCATTTCAATGTAGTCAATGTGACATGCGTTTCATACAG
  AAGTACCTGCTTCAGAGACATGAGAAGATTCATACTGGTGAAAAACCATTTCGCTGTGATGAATGTGGTA
  TGAGATTCATACAAAAATATCATATGGAAAGGCATAAGAGAACTCATAGTGGAGAAAAACCTTACCAGTG
  TGAATACTGTTTACAGTATTTTTCCAGAACAGATCGTGTATTGAAACATAAACGTATGTGCCATGAAAAT
  CATGACAAAAAACTAAATACATGTGCCATCAAAGGTGGCCTTCTGACATCTGAGGAAGATTCTGGCTTTT
  CTACATCACCAAAAGACAACTCACTGCCAAAAAAGAAAAGGCAGAAAACGGAGAAAAAATCATCTGGAAT
  GGACAAAGAGAGTGCTTTGGACAAATCTGACCTGAAAAAAGACAAAAATGATTACTTGCCTGTTTATTCT
  TCAAGTACTAAAGTAAAAGATGAGTATATGGTTGCAGAATATGCTGTTGAAATGCCACATTCGTCAGTTG
  GGGGCTCGCATTTAGAAGATGCGTCAGGAGAAATACACCCACCTAAGTTAGTTCTCAAAAAAATTAATAG
  TAAGAGAAGTCTGAAACAGCCACTGGAGCAAAATCAAACAATTTCACCTTTATCCACATATGAAGAGAGC
  AAAGTTTCAAAGTATGCTTTTGAACTTGTGGATAAACAGGCTTTACTGGACTCAGAAGGCAATGCTGACA
  TTGATCAGGTTGATAATTTGCAGGAGGGGCCCAGTAAACCTGTGCATAGTAGTACTAATTATGATGATGC
  CATGCAGTTTTTGAAGAAGAAGCGGTATCTTCAAGCAGCAAGTAACAACAGCAGGGAATATGCGCTGAAT
  GTGGGTACCATACGTTCTCAGCCTTCTGTAACACAAGCAGCTGTGGCAAGTGTCATTGATGAAAGTACCA
  CGGCATCCATATTAGAGTCACAGGCACTGAATGTGGAGATTAAGAGTAATCATGACAAAAATGTTATTCC
  AGATGAGGTACTGCAGACTCTGTTGGATCATTATTCCCACAAAGCTAATGGACAGCATGAGATATCCTTC
  AGTGTTGCAGATACTGAAGTGACTTCTAGCATATCAATAAATTCTTCAGAAGTACCAGAGGTCACCCCAT
  CAGAGAATGTTGGATCAAGCTCCCAAGCATCCTCATCAGATAAAGCCAACATGTTGCAGGAATACTCCAA
  GTTTCTGCAGCAGGCTTTGGACAGAACTAGCCAAAATGATGCCTATTTGAATAGCCCGAGCCTTAACTTT
  GTGACTGATAACCAGACCCTCCCAAATCAGCCAGCATTCTCTTCCATAGACAAGCAGGTCTATGCCACCA
  TGCCCATCAATAGCTTTCGATCAGGAATGAATTCTCCACTAAGAACAACTCCAGATAAGTCCCACTTTGG
  ACTAATAGTTGGTGATTCACAGCACTCATTTCCCTTTTCAGGTGATGAGACAAACCATGCTTCTGCCACA
  TCAACACAGGACTTTCTGGATCAAGTCACTTCTCAGAAGAAAGCTGAGGCCCAGCCTGTCCACCAAGCTT
  ACCAAATGAGCTCCTTTGAACAGCCCTTCCGTGCTCCCTATCATGGATCAAGAGCTGGAATAGCTACTCA
  ATTTAGCACTGCCAATGGACAGGTGAACCTTCGGGGACCAGGGACAAGTGCTGAATTTTCAGAATTTCCC
  TTGGTGAATGTAAATGATAATAGAGCTGGGATGACATCTTCACCTGATGCCACAACTGGCCAGACTTTTG
  GC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222687 representing NM_021964
  Red=Cloning site Green=Tags(s)
  
  MNIDDKLEGLFLKCGGIDEMQSSRTMVVMGGVSGQSTVSGELQDSVLQDRSMPHQEILAADEVLQESEMR
  QQDMISHDELMVHEETVKNDEEQMETHERLPQGLQYALNVPISVKQEITFTDVSEQLMRDKKQIREPVDL
  QKKKKRKQRSPAKILTINEDGSLGLKTPKSHVCEHCNAAFRTNYHLQRHVFIHTGEKPFQCSQCDMRFIQ
  KYLLQRHEKIHTGEKPFRCDECGMRFIQKYHMERHKRTHSGEKPYQCEYCLQYFSRTDRVLKHKRMCHEN
  HDKKLNTCAIKGGLLTSEEDSGFSTSPKDNSLPKKKRQKTEKKSSGMDKESALDKSDLKKDKNDYLPVYS
  SSTKVKDEYMVAEYAVEMPHSSVGGSHLEDASGEIHPPKLVLKKINSKRSLKQPLEQNQTISPLSTYEES
  KVSKYAFELVDKQALLDSEGNADIDQVDNLQEGPSKPVHSSTNYDDAMQFLKKKRYLQAASNNSREYALN
  VGTIRSQPSVTQAAVASVIDESTTASILESQALNVEIKSNHDKNVIPDEVLQTLLDHYSHKANGQHEISF
  SVADTEVTSSISINSSEVPEVTPSENVGSSSQASSSDKANMLQEYSKFLQQALDRTSQNDAYLNSPSLNF
  VTDNQTLPNQPAFSSIDKQVYATMPINSFRSGMNSPLRTTPDKSHFGLIVGDSQHSFPFSGDETNHASAT
  STQDFLDQVTSQKKAEAQPVHQAYQMSSFEQPFRAPYHGSRAGIATQFSTANGQVNLRGPGTSAEFSEFP
  LVNVNDNRAGMTSSPDATTGQTFG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021964
• ORF Size: 2382 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021964.1, NP_068799.1
• RefSeq Size: 3032 bp
• RefSeq ORF: 2385 bp
• Locus ID: 7707
• Cytogenetics: 3q21.2
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a member of the Kruppel family of zinc finger DNA binding proteins. The encoded protein activates transcription of the T-cell receptor and intestinal alkaline phosphatase genes but represses transcription of the ornithine decarboxylase, vimentin, gastrin, stomelysin, and enolase genes. Increased expression of this gene results in decreased patient survival rates from colorectal cancer, while mutations in this gene have been associated with global developmental delay, hypoplastic corpus callosum, and dysmorphic facies. [provided by RefSeq, Feb 2017]'