SF3B2 (NM_006842) Human Tagged ORF Clone

CAT#: RG222762

SF3B2 (GFP-tagged) - Human splicing factor 3b, subunit 2, 145kDa (SF3B2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SF3B2
• Synonyms: Cus1; SAP145; SF3b1; SF3B145; SF3b150
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222762 representing NM_006842
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGGAGCATCCCGAGCCTCCCAAAGCAGAATTGCAGCTGCCGCCGCCGCCACCTCCAGGCCACT
  ATGGCGCCTGGGCTGCCCAGGAGCTTCAGGCCAAGTTGGCAGAGATCGGAGCTCCGATCCAGGGTAATCG
  CGAGGAGCTGGTGGAGCGGCTGCAGAGCTACACCCGCCAGACTGGCATCGTGCTGAATCGGCCGGTTTTG
  AGAGGGGAAGATGGGGACAAAGCCGCTCCACCTCCCATGTCGGCACAGCTCCCTGGAATTCCCATGCCAC
  CACCACCTTTGGGACTCCCCCCTCTGCAGCCTCCTCCGCCACCCCCACCACCTCCACCAGGCCTTGGCCT
  TGGCTTTCCTATGGCCCACCCACCAAATTTGGGGCCCCCGCCTCCTCTCCGTGTGGGTGAGCCAGTGGCA
  CTGTCAGAGGAGGAGCGGCTGAAGTTGGCTCAGCAGCAGGCGGCATTGCTGATGCAGCAGGAGGAGCGTG
  CCAAGCAGCAGGGAGATCATTCGCTGAAGGAACATGAGCTCTTGGAGCAGCAGAAGCGGGCAGCTGTGTT
  ACTGGAGCAGGAACGACAGCAGGAGATTGCCAAGATGGGCACCCCAGTCCCTCGGCCCCCACAAGACATG
  GGCCAGATTGGTGTGCGCACTCCTCTGGGTCCTCGAGTAGCTGCTCCAGTGGGCCCAGTGGGCCCCACTC
  CTACAGTTTTGCCCATGGGAGCCCCTGTTCCCCGGCCTCGTGGTCCCCCACCGCCCCCTGGAGATGAGAA
  CAGAGAGATGGATGACCCCTCTGTGGGCCCCAAGATCCCCCAGGCTTTGGAGAAGATCCTGCAGCTGAAG
  GAGAGCCGCCAGGAAGAGATGAATTCTCAGCAGGAGGAAGAGGAAATGGAAACAGATGCTCGCTCGTCCC
  TGGGCCAGTCAGCGTCAGAGACTGAGGAGGACACAGTGTCCGTATCTAAAAAGGAGAAAAACCGGAAGCG
  TAGGAACCGAAAGAAGAAGAAAAAGCCCCAGCGGGTGCGAGGGGTGTCCTCTGAGAGCTCTGGGGACCGG
  GAGAAAGACTCAACCCGGTCCCGTGGCTCTGATTCCCCAGCAGCTGATGTTGAGATTGAGTATGTGACTG
  AAGAACCTGAAATTTACGAGCCCAACTTTATCTTCTTTAAGAGGATCTTTGAGGCTTTTAAGCTCACTGA
  TGATGTGAAGAAGGAGAAAGAGAAGGAGCCAGAGAAACTTGACAAACTGGAGAACTCTGCAGCCCCCAAG
  AAGAAGGGATTTGAAGAGGAGCACAAGGACAGTGATGATGACAGCAGTGATGACGAGCAGGAAAAGAAGC
  CAGAAGCCCCCAAGCTGTCCAAGAAGAAGTTGCGCCGAATGAACCGCTTCACTGTGGCTGAACTCAAGCA
  GCTGGTGGCTCGGCCCGATGTCGTGGAGATGCACGATGTGACAGCGCAGGACCCTAAGCTCTTGGTTCAC
  CTCAAGGCCACTCGGAACTCTGTGCCTGTGCCACGCCACTGGTGTTTTAAGCGCAAATACCTGCAGGGCA
  AACGGGGCATTGAGAAGCCCCCCTTCGAGCTGCCAGACTTCATCAAACGCACAGGCATCCAGGAGATGCG
  AGAGGCCCTGCAGGAGAAGGAAGAACAGAAGACCATGAAGTCAAAAATGCGAGAGAAAGTTCGGCCTAAG
  ATGGGCAAAATTGACATCGACTACCAGAAACTGCATGATGCCTTCTTCAAGTGGCAGACCAAGCCAAAGC
  TGACCATCCATGGGGACCTGTACTATGAGGGGAAGGAGTTCGAGACACGACTGAAGGAGAAGAAGCCAGG
  AGATCTGTCTGATGAGCTAAGGATTTCCTTGGGGATGCCAGTAGGACCAAATGCCCACAAGGTCCCTCCC
  CCATGGCTGATTGCCATGCAGCGATATGGACCACCCCCATCGTATCCCAACCTGAAAATCCCTGGGCTGA
  ACTCGCCCATCCCTGAGAGCTGTTCCTTTGGGTACCATGCTGGTGGCTGGGGCAAACCTCCAGTGGATGA
  GACTGGGAAACCGCTCTATGGGGACGTGTTTGGAACCAATGCTGCTGAATTTCAGACCAAGACTGAGGAA
  GAAGAGATTGATCGGACCCCTTGGGGGGAACTGGAACCATCTGATGAAGAATCCTCAGAAGAAGAGGAAG
  AGGAAGAAAGTGATGAAGACAAACCAGATGAGACAGGCTTTATTACCCCTGCAGACAGTGGCCTTATCAC
  TCCTGGAGGCTTTTCATCAGTGCCTGCTGGAATGGAGACCCCTGAACTCATTGAGCTGAGGAAGAAGAAG
  ATTGAGGAGGCGATGGACGGAAGTGAGACACCTCAGCTCTTCACTGTGTTGCCAGAGAAGAGAACAGCCA
  CTGTTGGAGGGGCCATGATGGGATCAACCCACATTTATGACATGTCCACGGTTATGAGCCGGAAGGGCCC
  GGCTCCTGAGCTGCAAGGTGTGGAAGTGGCGCTGGCGCCTGAAGAGTTGGAGCTGGATCCTATGGCCATG
  ACCCAGAAGTATGAGGAGCATGTGCGGGAGCAGCAGGCTCAAGTAGAGAAGGAGGACTTCAGTGACATGG
  TGGCTGAGCACGCTGCCAAACAGAAGCAAAAAAAACGGAAAGCTCAGCCCCAGGACAGCCGTGGGGGCAG
  CAAGAAATATAAGGAGTTCAAGTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222762 representing NM_006842
  Red=Cloning site Green=Tags(s)
  
  MATEHPEPPKAELQLPPPPPPGHYGAWAAQELQAKLAEIGAPIQGNREELVERLQSYTRQTGIVLNRPVL
  RGEDGDKAAPPPMSAQLPGIPMPPPPLGLPPLQPPPPPPPPPPGLGLGFPMAHPPNLGPPPPLRVGEPVA
  LSEEERLKLAQQQAALLMQQEERAKQQGDHSLKEHELLEQQKRAAVLLEQERQQEIAKMGTPVPRPPQDM
  GQIGVRTPLGPRVAAPVGPVGPTPTVLPMGAPVPRPRGPPPPPGDENREMDDPSVGPKIPQALEKILQLK
  ESRQEEMNSQQEEEEMETDARSSLGQSASETEEDTVSVSKKEKNRKRRNRKKKKKPQRVRGVSSESSGDR
  EKDSTRSRGSDSPAADVEIEYVTEEPEIYEPNFIFFKRIFEAFKLTDDVKKEKEKEPEKLDKLENSAAPK
  KKGFEEEHKDSDDDSSDDEQEKKPEAPKLSKKKLRRMNRFTVAELKQLVARPDVVEMHDVTAQDPKLLVH
  LKATRNSVPVPRHWCFKRKYLQGKRGIEKPPFELPDFIKRTGIQEMREALQEKEEQKTMKSKMREKVRPK
  MGKIDIDYQKLHDAFFKWQTKPKLTIHGDLYYEGKEFETRLKEKKPGDLSDELRISLGMPVGPNAHKVPP
  PWLIAMQRYGPPPSYPNLKIPGLNSPIPESCSFGYHAGGWGKPPVDETGKPLYGDVFGTNAAEFQTKTEE
  EEIDRTPWGELEPSDEESSEEEEEEESDEDKPDETGFITPADSGLITPGGFSSVPAGMETPELIELRKKK
  IEEAMDGSETPQLFTVLPEKRTATVGGAMMGSTHIYDMSTVMSRKGPAPELQGVEVALAPEELELDPMAM
  TQKYEEHVREQQAQVEKEDFSDMVAEHAAKQKQKKRKAQPQDSRGGSKKYKEFKF
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006842
• ORF Size: 2685 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006842.2, NP_006833.2
• RefSeq Size: 2902
• RefSeq ORF: 2688
• Locus ID: 10992
• Domains: SAP, PSP, DUF382
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus. [provided by RefSeq, Jul 2008]