CaV1.3 (CACNA1D) (NM_000720) Human Tagged ORF Clone

CAT#: RG222854

CACNA1D (GFP-tagged) - Human calcium channel, voltage-dependent, L type, alpha 1D subunit (CACNA1D), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: CACNA1D
• Synonyms: CACH3; CACN4; CACNL1A2; Cav1.3; CCHL1A2; PASNA; SANDD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222854 representing NM_000720
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGATGATGATGATGATGAAAAAAATGCAGCATCAACGGCAGCAGCAAGCGGACCACGCGAACGAGG
  CAAACTATGCAAGAGGCACCAGACTTCCTCTTTCTGGTGAAGGACCAACTTCTCAGCCGAATAGCTCCAA
  GCAAACTGTCCTGTCTTGGCAAGCTGCAATCGATGCTGCTAGACAGGCCAAGGCTGCCCAAACTATGAGC
  ACCTCTGCACCCCCACCTGTAGGATCTCTCTCCCAAAGAAAACGTCAGCAATACGCCAAGAGCAAAAAAC
  AGGGTAACTCGTCCAACAGCCGACCTGCCCGCGCCCTTTTCTGTTTATCACTCAATAACCCCATCCGAAG
  AGCCTGCATTAGTATAGTGGAATGGAAACCATTTGACATATTTATATTATTGGCTATTTTTGCCAATTGT
  GTGGCCTTAGCTATTTACATCCCATTCCCTGAAGATGATTCTAATTCAACAAATCATAACTTGGAAAAAG
  TAGAATATGCCTTCCTGATTATTTTTACAGTCGAGACATTTTTGAAGATTATAGCGTATGGATTATTGCT
  ACATCCTAATGCTTATGTTAGGAATGGATGGAATTTACTGGATTTTGTTATAGTAATAGTAGGATTGTTT
  AGTGTAATTTTGGAACAATTAACCAAAGAAACAGAAGGCGGGAACCACTCAAGCGGCAAATCTGGAGGCT
  TTGATGTCAAAGCCCTCCGTGCCTTTCGAGTGTTGCGACCACTTCGACTAGTGTCAGGGGTGCCCAGTTT
  ACAAGTTGTCCTGAACTCCATTATAAAAGCCATGGTTCCCCTCCTTCACATAGCCCTTTTGGTATTATTT
  GTAATCATAATCTATGCTATTATAGGATTGGAACTTTTTATTGGAAAAATGCACAAAACATGTTTTTTTG
  CTGACTCAGATATCGTAGCTGAAGAGGACCCAGCTCCATGTGCGTTCTCAGGGAATGGACGCCAGTGTAC
  TGCCAATGGCACGGAATGTAGGAGTGGCTGGGTTGGCCCGAACGGAGGCATCACCAACTTTGATAACTTT
  GCCTTTGCCATGCTTACTGTGTTTCAGTGCATCACCATGGAGGGCTGGACAGACGTGCTCTACTGGGTAA
  ATGATGCGATAGGATGGGAATGGCCATGGGTGTATTTTGTTAGTCTGATCATCCTTGGCTCATTTTTCGT
  CCTTAACCTGGTTCTTGGTGTCCTTAGTGGAGAATTCTCAAAGGAAAGAGAGAAGGCAAAAGCACGGGGA
  GATTTCCAGAAGCTCCGGGAGAAGCAGCAGCTGGAGGAGGATCTAAAGGGCTACTTGGATTGGATCACCC
  AAGCTGAGGACATCGATCCGGAGAATGAGGAAGAAGGAGGAGAGGAAGGCAAACGAAATACTAGCATGCC
  CACCAGCGAGACTGAGTCTGTGAACACAGAGAACGTCAGCGGTGAAGGCGAGAACCGAGGCTGCTGTGGA
  AGTCTCTGGTGCTGGTGGAGACGGAGAGGCGCGGCCAAGGCGGGGCCCTCTGGGTGTCGGCGGTGGGGTC
  AAGCCATCTCAAAATCCAAACTCAGCCGACGCTGGCGTCGCTGGAACCGATTCAATCGCAGAAGATGTAG
  GGCCGCCGTGAAGTCTGTCACGTTTTACTGGCTGGTTATCGTCCTGGTGTTTCTGAACACCTTAACCATT
  TCCTCTGAGCACTACAATCAGCCAGATTGGTTGACACAGATTCAAGATATTGCCAACAAAGTCCTCTTGG
  CTCTGTTCACCTGCGAGATGCTGGTAAAAATGTACAGCTTGGGCCTCCAAGCATATTTCGTCTCTCTTTT
  CAACCGGTTTGATTGCTTCGTGGTGTGTGGTGGAATCACTGAGACGATCCTGGTGGAACTGGAAATCATG
  TCTCCCCTGGGGATCTCTGTGTTTCGGTGTGTGCGCCTCTTAAGAATCTTCAAAGTGACCAGGCACTGGA
  CTTCCCTGAGCAACTTAGTGGCATCCTTATTAAACTCCATGAAGTCCATCGCTTCGCTGTTGCTTCTGCT
  TTTTCTCTTCATTATCATCTTTTCCTTGCTTGGGATGCAGCTGTTTGGCGGCAAGTTTAATTTTGATGAA
  ACGCAAACCAAGCGGAGCACCTTTGACAATTTCCCTCAAGCACTTCTCACAGTGTTCCAGATCCTGACAG
  GCGAAGACTGGAATGCTGTGATGTACGATGGCATCATGGCTTACGGGGGCCCATCCTCTTCAGGAATGAT
  CGTCTGCATCTACTTCATCATCCTCTTCATTTGTGGTAACTATATTCTACTGAATGTCTTCTTGGCCATC
  GCTGTAGACAATTTGGCTGATGCTGAAAGTCTGAACACTGCTCAGAAAGAAGAAGCGGAAGAAAAGGAGA
  GGAAAAAGATTGCCAGAAAAGAGAGCCTAGAAAATAAAAAGAACAACAAACCAGAAGTCAACCAGATAGC
  CAACAGTGACAACAAGGTTACAATTGATGACTATAGAGAAGAGGATGAAGACAAGGACCCCTATCCGCCT
  TGCGATGTGCCAGTAGGGGAAGAGGAAGAGGAAGAGGAGGAGGATGAACCTGAGGTTCCTGCCGGACCCC
  GTCCTCGAAGGATCTCGGAGTTGAACATGAAGGAAAAAATTGCCCCCATCCCTGAAGGGAGCGCTTTCTT
  CATTCTTAGCAAGACCAACCCGATCCGCGTAGGCTGCCACAAGCTCATCAACCACCACATCTTCACCAAC
  CTCATCCTTGTCTTCATCATGCTGAGCAGCGCTGCCCTGGCCGCAGAGGACCCCATCCGCAGCCACTCCT
  TCCGGAACACGATACTGGGTTACTTTGACTATGCCTTCACAGCCATCTTTACTGTTGAGATCCTGTTGAA
  GATGACAACTTTTGGAGCTTTCCTCCACAAAGGGGCCTTCTGCAGGAACTACTTCAATTTGCTGGATATG
  CTGGTGGTTGGGGTGTCTCTGGTGTCATTTGGGATTCAATCCAGTGCCATCTCCGTTGTGAAGATTCTGA
  GGGTCTTAAGGGTCCTGCGTCCCCTCAGGGCCATCAACAGAGCAAAAGGACTTAAGCACGTGGTCCAGTG
  CGTCTTCGTGGCCATCCGGACCATCGGCAACATCATGATCGTCACTACCCTCCTGCAGTTCATGTTTGCC
  TGTATCGGGGTCCAGTTGTTCAAGGGGAAGTTCTATCGCTGTACGGATGAAGCCAAAAGTAACCCTGAAG
  AATGCAGGGGACTTTTCATCCTCTACAAGGATGGGGATGTTGACAGTCCTGTGGTCCGTGAACGGATCTG
  GCAAAACAGTGATTTCAACTTCGACAACGTCCTCTCTGCTATGATGGCGCTCTTCACAGTCTCCACGTTT
  GAGGGCTGGCCTGCGTTGCTGTATAAAGCCATCGACTCGAATGGAGAGAACATCGGCCCAATCTACAACC
  ACCGCGTGGAGATCTCCATCTTCTTCATCATCTACATCATCATTGTAGCTTTCTTCATGATGAACATCTT
  TGTGGGCTTTGTCATCGTTACATTTCAGGAACAAGGAGAAAAAGAGTATAAGAACTGTGAGCTGGACAAA
  AATCAGCGTCAGTGTGTTGAATACGCCTTGAAAGCACGTCCCTTGCGGAGATACATCCCCAAAAACCCCT
  ACCAGTACAAGTTCTGGTACGTGGTGAACTCTTCGCCTTTCGAATACATGATGTTTGTCCTCATCATGCT
  CAACACACTCTGCTTGGCCATGCAGCACTACGAGCAGTCCAAGATGTTCAATGATGCCATGGACATTCTG
  AACATGGTCTTCACCGGGGTGTTCACCGTCGAGATGGTTTTGAAAGTCATCGCATTTAAGCCTAAGGGGT
  ATTTTAGTGACGCCTGGAACACGTTTGACTCCCTCATCGTAATCGGCAGCATTATAGACGTGGCCCTCAG
  CGAAGCGGACCCAACTGAAAGTGAAAATGTCCCTGTCCCAACTGCTACACCTGGGAACTCTGAAGAGAGC
  AATAGAATCTCCATCACCTTTTTCCGTCTTTTCCGAGTGATGCGATTGGTGAAGCTTCTCAGCAGGGGGG
  AAGGCATCCGGACATTGCTGTGGACTTTTATTAAGTCCTTTCAGGCGCTCCCGTATGTGGCCCTCCTCAT
  AGCCATGCTGTTCTTCATCTATGCGGTCATTGGCATGCAGATGTTTGGGAAAGTTGCCATGAGAGATAAC
  AACCAGATCAATAGGAACAATAACTTCCAGACGTTTCCCCAGGCGGTGCTGCTGCTCTTCAGGTGTGCAA
  CAGGTGAGGCCTGGCAGGAGATCATGCTGGCCTGTCTCCCAGGGAAGCTCTGTGACCCTGAGTCAGATTA
  CAACCCCGGGGAGGAGTATACATGTGGGAGCAACTTTGCCATTGTCTATTTCATCAGTTTTTACATGCTC
  TGTGCATTTCTGATCATCAATCTGTTTGTGGCTGTCATCATGGATAATTTCGACTATCTGACCCGGGACT
  GGTCTATTTTGGGGCCTCACCATTTAGATGAATTCAAAAGAATATGGTCAGAATATGACCCTGAGGCAAA
  GGGAAGGATAAAACACCTTGATGTGGTCACTCTGCTTCGACGCATCCAGCCTCCCCTGGGGTTTGGGAAG
  TTATGTCCACACAGGGTAGCGTGCAAGAGATTAGTTGCCATGAACATGCCTCTCAACAGTGACGGGACAG
  TCATGTTTAATGCAACCCTGTTTGCTTTGGTTCGAACGGCTCTTAAGATCAAGACCGAAGGGAACCTGGA
  GCAAGCTAATGAAGAACTTCGGGCTGTGATAAAGAAAATTTGGAAGAAAACCAGCATGAAATTACTTGAC
  CAAGTTGTCCCTCCAGCTGGTGATGATGAGGTAACCGTGGGGAAGTTCTATGCCACTTTCCTGATACAGG
  ACTACTTTAGGAAATTCAAGAAACGGAAAGAACAAGGACTGGTGGGAAAGTACCCTGCGAAGAACACCAC
  AATTGCCCTACAGGCGGGATTAAGGACACTGCATGACATTGGGCCAGAAATCCGGCGTGCTATATCGTGT
  GATTTGCAAGATGACGAGCCTGAGGAAACAAAACGAGAAGAAGAAGATGATGTGTTCAAAAGAAATGGTG
  CCCTGCTTGGAAACCATGTCAATCATGTTAATAGTGATAGGAGAGATTCCCTTCAGCAGACCAATACCAC
  CCACCGTCCCCTGCATGTCCAAAGGCCTTCAATTCCACCTGCAAGTGATACTGAGAAACCGCTGTTTCCT
  CCAGCAGGAAATTCGGTGTGTCATAACCATCATAACCATAATTCCATAGGAAAGCAAGTTCCCACCTCAA
  CAAATGCCAATCTCAATAATGCCAATATGTCCAAAGCTGCCCATGGAAAGCGGCCCAGCATTGGGAACCT
  TGAGCATGTGTCTGAAAATGGGCATCATTCTTCCCACAAGCATGACCGGGAGCCTCAGAGAAGGTCCAGT
  GTGAAAAGAACCCGCTATTATGAAACTTACATTAGGTCCGACTCAGGAGATGAACAGCTCCCAACTATTT
  GCCGGGAAGACCCAGAGATACATGGCTATTTCAGGGACCCCCACTGCTTGGGGGAGCAGGAGTATTTCAG
  TAGTGAGGAATGCTACGAGGATGACAGCTCGCCCACCTGGAGCAGGCAAAACTATGGCTACTACAGCAGA
  TACCCAGGCAGAAACATCGACTCTGAGAGGCCCCGAGGCTACCATCATCCCCAAGGATTCTTGGAGGACG
  ATGACTCGCCCGTTTGCTATGATTCACGGAGATCTCCAAGGAGACGCCTACTACCTCCCACCCCAGCATC
  CCACCGGAGATCCTCCTTCAACTTTGAGTGCCTGCGCCGGCAGAGCAGCCAGGAAGAGGTCCCGTCGTCT
  CCCATCTTCCCCCATCGCACGGCCCTGCCTCTGCATCTAATGCAGCAACAGATCATGGCAGTTGCCGGCC
  TAGATTCAAGTAAAGCCCAGAAGTACTCACCGAGTCACTCGACCCGGTCGTGGGCCACCCCTCCAGCAAC
  CCCTCCCTACCGGGACTGGACACCGTGCTACACCCCCCTGATCCAAGTGGAGCAGTCAGAGGCCCTGGAC
  CAGGTGAACGGCAGCCTGCCGTCCCTGCACCGCAGCTCCTGGTACACAGACGAGCCCGACATCTCCTACC
  GGACTTTCACACCAGCCAGCCTGACTGTCCCCAGCAGCTTCCGGAACAAAAACAGCGACAAGCAGAGGAG
  TGCGGACAGCTTGGTGGAGGCAGTCCTGATATCCGAAGGCTTGGGACGCTATGCAAGGGACCCAAAATTT
  GTGTCAGCAACAAAACACGAAATCGCTGATGCCTGTGACCTCACCATCGACGAGATGGAGAGTGCAGCCA
  GCACCCTGCTTAATGGGAACGTGCGTCCCCGAGCCAACGGGGATGTGGGCCCCCTCTCACACCGGCAGGA
  CTATGAGCTACAGGACTTTGGTCCTGGCTACAGCGACGAAGAGCCAGACCCTGGGAGGGATGAGGAGGAC
  CTGGCGGATGAAATGATATGCATCACCACCTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222854 representing NM_000720
  Red=Cloning site Green=Tags(s)
  
  MMMMMMMKKMQHQRQQQADHANEANYARGTRLPLSGEGPTSQPNSSKQTVLSWQAAIDAARQAKAAQTMS
  TSAPPPVGSLSQRKRQQYAKSKKQGNSSNSRPARALFCLSLNNPIRRACISIVEWKPFDIFILLAIFANC
  VALAIYIPFPEDDSNSTNHNLEKVEYAFLIIFTVETFLKIIAYGLLLHPNAYVRNGWNLLDFVIVIVGLF
  SVILEQLTKETEGGNHSSGKSGGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLF
  VIIIYAIIGLELFIGKMHKTCFFADSDIVAEEDPAPCAFSGNGRQCTANGTECRSGWVGPNGGITNFDNF
  AFAMLTVFQCITMEGWTDVLYWVNDAIGWEWPWVYFVSLIILGSFFVLNLVLGVLSGEFSKEREKAKARG
  DFQKLREKQQLEEDLKGYLDWITQAEDIDPENEEEGGEEGKRNTSMPTSETESVNTENVSGEGENRGCCG
  SLWCWWRRRGAAKAGPSGCRRWGQAISKSKLSRRWRRWNRFNRRRCRAAVKSVTFYWLVIVLVFLNTLTI
  SSEHYNQPDWLTQIQDIANKVLLALFTCEMLVKMYSLGLQAYFVSLFNRFDCFVVCGGITETILVELEIM
  SPLGISVFRCVRLLRIFKVTRHWTSLSNLVASLLNSMKSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDE
  TQTKRSTFDNFPQALLTVFQILTGEDWNAVMYDGIMAYGGPSSSGMIVCIYFIILFICGNYILLNVFLAI
  AVDNLADAESLNTAQKEEAEEKERKKIARKESLENKKNNKPEVNQIANSDNKVTIDDYREEDEDKDPYPP
  CDVPVGEEEEEEEEDEPEVPAGPRPRRISELNMKEKIAPIPEGSAFFILSKTNPIRVGCHKLINHHIFTN
  LILVFIMLSSAALAAEDPIRSHSFRNTILGYFDYAFTAIFTVEILLKMTTFGAFLHKGAFCRNYFNLLDM
  LVVGVSLVSFGIQSSAISVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIMIVTTLLQFMFA
  CIGVQLFKGKFYRCTDEAKSNPEECRGLFILYKDGDVDSPVVRERIWQNSDFNFDNVLSAMMALFTVSTF
  EGWPALLYKAIDSNGENIGPIYNHRVEISIFFIIYIIIVAFFMMNIFVGFVIVTFQEQGEKEYKNCELDK
  NQRQCVEYALKARPLRRYIPKNPYQYKFWYVVNSSPFEYMMFVLIMLNTLCLAMQHYEQSKMFNDAMDIL
  NMVFTGVFTVEMVLKVIAFKPKGYFSDAWNTFDSLIVIGSIIDVALSEADPTESENVPVPTATPGNSEES
  NRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIAMLFFIYAVIGMQMFGKVAMRDN
  NQINRNNNFQTFPQAVLLLFRCATGEAWQEIMLACLPGKLCDPESDYNPGEEYTCGSNFAIVYFISFYML
  CAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWSEYDPEAKGRIKHLDVVTLLRRIQPPLGFGK
  LCPHRVACKRLVAMNMPLNSDGTVMFNATLFALVRTALKIKTEGNLEQANEELRAVIKKIWKKTSMKLLD
  QVVPPAGDDEVTVGKFYATFLIQDYFRKFKKRKEQGLVGKYPAKNTTIALQAGLRTLHDIGPEIRRAISC
  DLQDDEPEETKREEEDDVFKRNGALLGNHVNHVNSDRRDSLQQTNTTHRPLHVQRPSIPPASDTEKPLFP
  PAGNSVCHNHHNHNSIGKQVPTSTNANLNNANMSKAAHGKRPSIGNLEHVSENGHHSSHKHDREPQRRSS
  VKRTRYYETYIRSDSGDEQLPTICREDPEIHGYFRDPHCLGEQEYFSSEECYEDDSSPTWSRQNYGYYSR
  YPGRNIDSERPRGYHHPQGFLEDDDSPVCYDSRRSPRRRLLPPTPASHRRSSFNFECLRRQSSQEEVPSS
  PIFPHRTALPLHLMQQQIMAVAGLDSSKAQKYSPSHSTRSWATPPATPPYRDWTPCYTPLIQVEQSEALD
  QVNGSLPSLHRSSWYTDEPDISYRTFTPASLTVPSSFRNKNSDKQRSADSLVEAVLISEGLGRYARDPKF
  VSATKHEIADACDLTIDEMESAASTLLNGNVRPRANGDVGPLSHRQDYELQDFGPGYSDEEPDPGRDEED
  LADEMICITTL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000720
• ORF Size: 6543 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000720.1, NP_000711.1
• RefSeq Size: 7193 bp
• RefSeq ORF: 6546 bp
• Locus ID: 776
• Cytogenetics: 3p21.1
• Domains: ion_trans
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Alzheimer's disease, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Calcium signaling pathway, Cardiac muscle contraction, Dilated cardiomyopathy, GnRH signaling pathway, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Type II diabetes mellitus, Vascular smooth muscle contraction
• Gene Summary: 'Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, namely alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1D subunit. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]'