Bone Sialoprotein (IBSP) (NM_004967) Human Tagged ORF Clone

CAT#: RG222901

IBSP (GFP-tagged) - Human integrin-binding sialoprotein (IBSP)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: IBSP
• Synonyms: BNSP; BSP; BSP-II; SP-II
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG222901 representing NM_004967
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACTGCTTTAATTTTGCTCAGCATTTTGGGAATGGCCTGTGCTTTCTCAATGAAAAATTTGCATC
  GAAGAGTCAAAATAGAGGATTCTGAAGAAAATGGGGTCTTTAAGTACAGGCCACGATATTATCTTTACAA
  GCATGCCTACTTTTATCCTCATTTAAAACGATTTCCAGTTCAGGGCAGTAGTGACTCATCCGAAGAAAAT
  GGAGATGACAGTTCAGAAGAGGAGGAGGAAGAAGAGGAGACTTCAAATGAAGGAGAAAACAATGAAGAAT
  CGAATGAAGATGAAGACTCTGAGGCTGAGAATACCACACTTTCTGCTACAACACTGGGCTATGGAGAGGA
  CGCCACGCCTGGCACAGGGTATACAGGGTTAGCTGCAATCCAGCTTCCCAAGAAGGCTGGGGATATAACA
  AACAAAGCTACAAAAGAGAAGGAAAGTGATGAAGAAGAAGAGGAGGAAGAGGAAGGAAATGAAAACGAAG
  AAAGCGAAGCAGAAGTGGATGAAAACGAACAAGGCATAAACGGCACCAGTACCAACAGCACAGAGGCAGA
  AAACGGCAACGGCAGCAGCGGAGGAGACAATGGAGAAGAAGGGGAAGAAGAAAGTGTCACTGGAGCCAAT
  GCAGAAGGCACCACAGAGACCGGAGGGCAGGGCAAGGGCACCTCGAAGACAACAACCTCTCCAAATGGTG
  GGTTTGAACCTACAACCCCACCACAAGTCTATAGAACCACTTCCCCACCTTTTGGGAAAACCACCACCGT
  TGAATACGAGGGGGAGTACGAATACACGGGCGTCAATGAATACGACAATGGATATGAAATCTATGAAAGT
  GAGAACGGGGAACCTCGTGGGGACAATTACCGAGCCTATGAAGATGAGTACAGCTACTTTAAAGGACAAG
  GCTACGATGGCTATGATGGTCAGAATTACTACCACCACCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG222901 representing NM_004967
  Red=Cloning site Green=Tags(s)
  
  MKTALILLSILGMACAFSMKNLHRRVKIEDSEENGVFKYRPRYYLYKHAYFYPHLKRFPVQGSSDSSEEN
  GDDSSEEEEEEEETSNEGENNEESNEDEDSEAENTTLSATTLGYGEDATPGTGYTGLAAIQLPKKAGDIT
  NKATKEKESDEEEEEEEEGNENEESEAEVDENEQGINGTSTNSTEAENGNGSSGGDNGEEGEEESVTGAN
  AEGTTETGGQGKGTSKTTTSPNGGFEPTTPPQVYRTTSPPFGKTTTVEYEGEYEYTGVNEYDNGYEIYES
  ENGEPRGDNYRAYEDEYSYFKGQGYDGYDGQNYYHHQ
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004967
• ORF Size: 951 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004967.2, NP_004958.1
• RefSeq Size: 1108
• RefSeq ORF: 954
• Locus ID: 3381
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion
• Gene Summary: The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. [provided by RefSeq, Jul 2008]