LDB3 (NM_007078) Human Tagged ORF Clone

CAT#: RG223063

LDB3 (GFP-tagged) - Human LIM domain binding 3 (LDB3), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: LDB3
• Synonyms: CMD1C; CMH24; CMPD3; CYPHER; LDB3Z1; LDB3Z4; LVNC3; MFM4; ORACLE; PDLIM6; ZASP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223063 representing NM_007078
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTACAGTGTGACCCTGACTGGGCCCGGGCCCTGGGGCTTCCGTCTGCAGGGGGGCAAGGACTTCA
  ACATGCCCCTCACTATCTCCCGGATCACACCAGGCAGCAAGGCAGCCCAGTCCCAGCTCAGCCAGGGTGA
  CCTCGTGGTGGCCATTGACGGCGTCAACACAGACACCATGACCCACCTGGAAGCCCAGAACAAGATCAAG
  TCTGCCAGCTACAACTTGAGCCTCACCCTGCAGAAATCAAAGCGTCCCATTCCCATCTCCACGACAGCAC
  CTCCAGTCCAGACCCCTCTGCCGGTGATCCCTCACCAGAAGGACCCCGCTCTGGACACGAACGGCAGCCT
  GGTGGCACCCAGCCCCAGCCCTGAGGCGAGGGCCAGCCCAGGCACCCCAGGCACCCCGGAGCTCAGGCCC
  ACCTTTAGCCCTGCCTTCTCCCGGCCCTCCGCCTTCTCCTCACTCGCCGAGGCCTCTGACCCTGGCCCTC
  CGCGGGCCAGCCTGAGGGCCAAGACCAGCCCAGAGGGGGCCCGGGACCTACTCGGCCCAAAAGCCCTGCC
  GGGCTCGAGCCAGCCGAGGCAATATAACAACCCCATTGGCCTGTACTCGGCAGAGACCCTGAGGGAGATG
  GCTCAGATGTACCAGATGAGCCTCCGAGGGAAGGCCTCGGGTGTCGGACTCCCAGGAGGGAGCCTCCCTA
  TTAAGGACCTTGCCGTAGACAGCGCCTCTCCCGTCTACCAGGCTGTGATTAAGAGCCAGAACAAGCCAGA
  AGATGAGGCTGACGAGTGGGCACGCCGTTCCTCCAACCTGCAGTCTCGCTCCTTCCGCATCCTGGCCCAG
  ATGACGGGGACAGAATTCATGCAAGACCCTGATGAAGAAGCTCTGCGAAGGTCAAGCACCCCTATTGAGC
  ATGCGCCGGTGTGCACCAGCCAGGCCACCACCCCGCTGCTGCCCGCTTCTGCCCAGCCACCTGCTGCTGC
  CTCTCCCAGTGCGGCTTCGCCACCCCTGGCCACAGCTGCTGCCCACACTGCCATCGCCTCCGCCTCCACC
  ACAGCCCCTGCTTCAAGTCCTGCCGACAGCCCAAGGCCCCAGGCCTCTTCCTACAGCCCCGCAGTGGCCG
  CCTCTTCAGCACCTGCCACCCACACCAGCTACAGTGAGGGCCCCGCCGCCCCTGCACCCAAGCCCCGGGT
  TGTCACCACTGCCAGCATCCGGCCTTCTGTCTACCAGCCAGTGCCTGCATCTACCTACAGCCCGTCCCCA
  GGGGCCAATTACAGTCCCACTCCCTACACCCCCTCCCCTGCCCCTGCCTACACCCCCTCCCCTGCCCCTG
  CCTACACCCCCTCACCTGTCCCCACCTACACTCCATCCCCAGCACCAGCCTATACCCCCTCACCTGCCCC
  CAACTATAACCCTGCACCCTCGGTGGCCTACAGCGGGGGCCCTGCGGAGCCTGCCAGCCGTCCACCCTGG
  GTGACAGATGATAGCTTCTCCCAGAAGTTTGCCCCGGGCAAGAGCACCACCTCCATCAGCAAGCAGACCC
  TGCCCCGGGGAGGCCCAGCCTACACCCCAGCGGGTCCTCAGGTGCCACCACTTGCCAGGGGGACCGTCCA
  GAGGGCTGAGCGATTCCCAGCCAGCAGCCGGACTCCACTCTGCGGTCACTGCAACAATGTCATCCGGGGC
  CCATTTCTGGTAGCCATGGGCCGTTCTTGGCACCCTGAAGAGTTCACCTGTGCCTACTGCAAGACTTCCC
  TGGCAGATGTGTGCTTTGTGGAAGAGCAGAACAACGTTTACTGTGAGCGATGTTATGAGCAATTCTTTGC
  CCCGCTGTGTGCCAAGTGCAACACCAAAATTATGGGGGAAGTAATGCATGCCTTGAGACAGACATGGCAC
  ACCACCTGCTTCGTCTGTGCGGCCTGCAAGAAGCCTTTTGGGAACAGCCTCTTCCACATGGAAGACGGGG
  AGCCCTACTGCGAGAAAGACTACATCAATCTGTTCAGCACCAAGTGCCATGGCTGCGATTTCCCCGTGGA
  GGCTGGCGACAAGTTTATCGAAGCCCTGGGCCACACTTGGCACGACACCTGCTTCATTTGCGCAGTCTGC
  CATGTGAATCTGGAGGGGCAGCCGTTCTACTCCAAGAAGGACAGACCCCTGTGCAAGAAGCACGCACACA
  CCATCAACTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223063 representing NM_007078
  Red=Cloning site Green=Tags(s)
  
  MSYSVTLTGPGPWGFRLQGGKDFNMPLTISRITPGSKAAQSQLSQGDLVVAIDGVNTDTMTHLEAQNKIK
  SASYNLSLTLQKSKRPIPISTTAPPVQTPLPVIPHQKDPALDTNGSLVAPSPSPEARASPGTPGTPELRP
  TFSPAFSRPSAFSSLAEASDPGPPRASLRAKTSPEGARDLLGPKALPGSSQPRQYNNPIGLYSAETLREM
  AQMYQMSLRGKASGVGLPGGSLPIKDLAVDSASPVYQAVIKSQNKPEDEADEWARRSSNLQSRSFRILAQ
  MTGTEFMQDPDEEALRRSSTPIEHAPVCTSQATTPLLPASAQPPAAASPSAASPPLATAAAHTAIASAST
  TAPASSPADSPRPQASSYSPAVAASSAPATHTSYSEGPAAPAPKPRVVTTASIRPSVYQPVPASTYSPSP
  GANYSPTPYTPSPAPAYTPSPAPAYTPSPVPTYTPSPAPAYTPSPAPNYNPAPSVAYSGGPAEPASRPPW
  VTDDSFSQKFAPGKSTTSISKQTLPRGGPAYTPAGPQVPPLARGTVQRAERFPASSRTPLCGHCNNVIRG
  PFLVAMGRSWHPEEFTCAYCKTSLADVCFVEEQNNVYCERCYEQFFAPLCAKCNTKIMGEVMHALRQTWH
  TTCFVCAACKKPFGNSLFHMEDGEPYCEKDYINLFSTKCHGCDFPVEAGDKFIEALGHTWHDTCFICAVC
  HVNLEGQPFYSKKDRPLCKKHAHTINL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007078
• ORF Size: 2181 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007078.2, NP_009009.1
• RefSeq Size: 5308
• RefSeq ORF: 2184
• Locus ID: 11155
• Gene Summary: This gene encodes a PDZ domain-containing protein. PDZ motifs are modular protein-protein interaction domains consisting of 80-120 amino acid residues. PDZ domain-containing proteins interact with each other in cytoskeletal assembly or with other proteins involved in targeting and clustering of membrane proteins. The protein encoded by this gene interacts with alpha-actinin-2 through its N-terminal PDZ domain and with protein kinase C via its C-terminal LIM domains. The LIM domain is a cysteine-rich motif defined by 50-60 amino acids containing two zinc-binding modules. This protein also interacts with all three members of the myozenin family. Mutations in this gene have been associated with myofibrillar myopathy and dilated cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been identified; all isoforms have N-terminal PDZ domains while only longer isoforms (1, 2 and 5) have C-terminal LIM domains. [provided by RefSeq, Jan 2010]