LPHN1 (ADGRL1) (NM_001008701) Human Tagged ORF Clone

CAT#: RG223160

LPHN1 (GFP-tagged) - Human latrophilin 1 (LPHN1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADGRL1
• Synonyms: CIRL1; CL1; LEC2; LPHN1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223160 representing NM_001008701
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCGCCTAGCCGCAGTGCTCTGGAATCTGTGTGTCACCGCCGTCCTGGTCACCTCGGCCACCCAAG
  GCCTGAGCCGGGCCGGGCTCCCGTTCGGGCTGATGCGCCGGGAGCTGGCGTGTGAAGGCTACCCCATCGA
  GCTGCGGTGCCCCGGCAGCGACGTCATCATGGTGGAGAATGCCAACTACGGGCGCACGGACGACAAGATT
  TGCGATGCTGACCCTTTCCAGATGGAGAATGTGCAGTGCTACCTGCCGGACGCCTTCAAGATCATGTCAC
  AGAGGTGTAACAACCGCACCCAGTGCGTGGTGGTCGCCGGCTCGGATGCCTTTCCTGACCCCTGTCCTGG
  GACCTACAAGTACCTGGAGGTGCAGTACGACTGTGTCCCCTACAAAGTGGAGCAGAAAGTCTTCGTGTGC
  CCAGGGACCCTGCAGAAGGTGCTGGAGCCCACCTCGACACACGAGTCAGAGCACCAGTCTGGCGCATGGT
  GCAAGGACCCGCTGCAGGCGGGTGACCGCATCTACGTGATGCCCTGGATCCCCTACCGCACGGACACACT
  GACTGAGTATGCCTCGTGGGAGGACTACGTGGCCGCCCGCCACACCACCACCTACCGCCTGCCCAACCGC
  GTGGATGGCACAGGCTTTGTGGTCTACGATGGTGCCGTCTTCTACAACAAGGAGCGCACGCGCAACATCG
  TCAAGTATGACCTACGGACACGCATCAAGAGCGGGGAGACGGTCATCAATACCGCCAACTACCATGACAC
  CTCGCCCTACCGCTGGGGCGGAAAGACCGACATTGACCTGGCGGTGGACGAGAACGGGCTGTGGGTCATC
  TACGCCACTGAGGGCAACAACGGGCGGCTGGTGGTGAGCCAGCTGAACCCCTACACACTGCGCTTTGAGG
  GCACGTGGGAGACGGGTTACGACAAGCGCTCGGCATCCAACGCCTTCATGGTGTGTGGGGTCCTGTACGT
  CCTGCGTTCCGTGTACGTGGATGATGACAGCGAGGCGGCTGGCAACCGCGTGGACTATGCCTTCAACACC
  AATGCCAACCGCGAGGAGCCTGTCAGCCTCACCTTCCCCAACCCCTACCAGTTCATCTCCTCCGTTGACT
  ACAACCCTCGCGACAACCAGCTGTACGTCTGGAACAACTATTTCGTGGTGCGCTACAGCCTGGAGTTCGG
  GCCGCCCGACCCCAGTGCTGGCCCAGCCACTTCCCCACCCCTCAGCACGACCACCACAGCCAGGCCCACG
  CCCCTCACCAGCACAGCCTCGCCCGCAGCCACCACCCCGCTCCGCCGGGCACCCCTCACCACGCACCCAG
  TGGGTGCCATCAACCAGCTGGGACCTGATCTGCCTCCAGCCACAGCCCCAGTCCCCAGCACCCGGCGGCC
  CCCAGCCCCGAATCTACACGTGTCCCCTGAGCTCTTCTGCGAGCCCCGAGAGGTACGGCGGGTCCAGTGG
  CCGGCCACCCAGCAGGGCATGCTGGTGGAGAGGCCCTGCCCCAAGGGGACTCGAGGAATTGCCTCCTTCC
  AGTGTCTACCAGCCTTGGGGCTCTGGAACCCCCGGGGCCCTGACCTCAGCAACTGCACCTCCCCCTGGGT
  CAACCAGGTGGCCCAGAAGATCAAGAGTGGGGAGAACGCGGCCAACATCGCCAGCGAGCTGGCCCGACAC
  ACCCGGGGCTCCATCTACGCGGGGGACGTCTCCTCCTCTGTGAAGCTGATGGAGCAGCTGCTGGACATCC
  TGGATGCCCAGCTGCAGGCCCTGCGGCCCATCGAGCGCGAGTCAGCCGGCAAGAACTACAACAAGATGCA
  CAAGCGAGAGAGAACTTGTAAGGATTATATCAAGGCCGTGGTGGAGACAGTGGACAATCTGCTCCGGCCA
  GAAGCTCTGGAGTCCTGGAAGGACATGAATGCCACGGAGCAGGTGCACACGGCCACCATGCTCCTCGACG
  TCCTGGAGGAGGGCGCCTTCCTGCTGGCCGACAATGTCAGGGAGCCTGCCCGCTTCCTGGCTGCCAAGGA
  GAACGTGGTCCTGGAGGTCACAGTCCTGAACACAGAGGGCCAGGTGCAGGAGCTGGTGTTCCCCCAGGAG
  GAGTACCCGAGAAAGAACTCCATCCAGCTGTCTGCCAAAACCATCAAGCAGAACAGCCGCAATGGGGTGG
  TCAAAGTTGTCTTCATCCTCTACAACAACCTGGGCCTCTTCCTGTCCACGGAGAATGCCACAGTGAAGCT
  GGCCGGCGAAGCAGGCCCGGGTGGCCCTGGGGGCGCCTCTCTAGTGGTGAACTCACAGGTCATCGCAGCA
  TCCATCAACAAGGAGTCCAGCCGCGTCTTCCTCATGGACCCTGTCATCTTCACCGTGGCCCACCTGGAGG
  ACAAGAACCACTTCAATGCTAACTGCTCCTTCTGGAACTACTCGGAGCGTTCCATGCTGGGCTACTGGTC
  GACCCAAGGCTGCCGCCTGGTGGAGTCCAACAAGACCCATACCACGTGTGCCTGCAGCCACCTCACCAAC
  TTCGCTGTGCTCATGGCTCACCGTGAGATCTACCAGGGCCGCATCAACGAGCTGCTGCTGTCGGTCATCA
  CCTGGGTGGGCATTGTGATCTCCCTGGTCTGCTTGGCCATCTGCATCTCCACCTTCTGCTTCCTGCGGGG
  GCTGCAGACCGACCGCAACACCATCCACAAGAACCTGTGCATCAACCTCTTCCTGGCTGAGCTGCTCTTC
  CTGGTCGGGATCGACAAGACTCAGTATGAGATTGCCTGCCCCATCTTCGCCGGCCTGCTGCACTATTTCT
  TCCTGGCTGCCTTCTCCTGGCTGTGCCTGGAGGGCGTGCACCTCTACCTGCTACTAGTGGAGGTGTTTGA
  GAGCGAGTATTCCCGCACCAAGTACTACTACCTGGGTGGCTACTGCTTCCCGGCCCTGGTGGTGGGCATC
  GCGGCTGCCATTGACTACCGCAGCTACGGCACCGAGAAGGCCTGCTGGCTCCGAGTGGACAATTACTTCA
  TCTGGAGTTTCATCGGGCCAGTCTCCTTCGTTATCGTGGTCAACCTGGTGTTCCTCATGGTGACCCTGCA
  CAAGATGATCCGAAGCTCATCTGTGCTCAAGCCCGACTCCAGCCGCCTGGACAACATTAAATCCTGGGCG
  CTGGGGGCCATCGCGCTGCTGTTCCTGCTGGGCCTCACCTGGGCTTTCGGCCTCCTCTTCATCAACAAGG
  AGTCGGTGGTCATGGCCTATCTCTTCACCACCTTCAACGCCTTCCAGGGGGTCTTCATCTTCGTCTTTCA
  CTGCGCCTTACAGAAGAAGGTGCACAAGGAGTACAGCAAGTGCCTGCGTCACTCCTACTGCTGCATCCGC
  TCCCCACCCGGGGGCACTCACGGATCCCTCAAGACCTCAGCCATGCGAAGCAACACCCGCTACTACACAG
  GGACCCAGAGCCGAATTCGGAGGATGTGGAATGACACTGTGAGGAAACAGACGGAGTCCTCCTTCATGGC
  GGGTGACATCAACAGCACCCCCACCCTGAACCGAGGTACCATGGGGAACCACCTGCTGACCAACCCCGTG
  CTGCAGCCCCGTGGGGGCACCAGTCCCTACAACACCCTCATCGCCGAGTCAGTGGGCTTCAATCCCTCCT
  CGCCCCCTGTCTTCAACTCCCCAGGGAGCTACCGGGAACCCAAGCACCCCTTGGGAGGCCGGGAAGCCTG
  TGGCATGGACACCCTGCCCCTGAACGGCAACTTCAATAACAGTTACTCCTTGCGAAGTGGGGATTTCCCT
  CCCGGGGATGGGGGCCCTGAGCCGCCCCGAGGCCGGAACCTAGCCGATGCGGCGGCCTTTGAGAAGATGA
  TCATCTCAGAGCTGGTGCACAACAACCTGCGGGGGAGCAGCAGCGCGGCCAAGGGCCCTCCACCGCCTGA
  GCCCCCTGTGCCACCTGTGCCAGGGGGCGGGGGCGAGGAAGAGGCGGGCGGGCCCGGGGGTGCTGACCGG
  GCCGAGATTGAACTTCTCTATAAGGCCCTGGAGGAGCCTCTGCTGCTGCCCCGGGCCCAGTCGGTGCTGT
  ACCAGAGCGATCTGGACGAGTCGGAGAGCTGCACGGCCGAGGACGGCGCCACCAGCCGGCCCCTCTCCTC
  CCCTCCTGGCCGGGACTCCCTCTATGCCAGCGGGGCCAACCTGCGGGACTCACCCTCCTACCCGGACAGC
  AGCCCTGAGGGGCCCAGTGAGGCCCTGCCCCCACCCCCTCCCGCACCCCCCGGCCCCCCCGAAATCTACT
  ACACCTCGCGCCCGCCAGCCCTGGTGGCCCGGAATCCCCTGCAGGGCTACTACCAGGTGCGGCGTCCTAG
  CCACGAGGGCTACCTGGCAGCCCCAGGCCTTGAGGGGCCAGGGCCCGATGGGGACGGGCAGATGCAGCTG
  GTCACCAGTCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223160 representing NM_001008701
  Red=Cloning site Green=Tags(s)
  
  MARLAAVLWNLCVTAVLVTSATQGLSRAGLPFGLMRRELACEGYPIELRCPGSDVIMVENANYGRTDDKI
  CDADPFQMENVQCYLPDAFKIMSQRCNNRTQCVVVAGSDAFPDPCPGTYKYLEVQYDCVPYKVEQKVFVC
  PGTLQKVLEPTSTHESEHQSGAWCKDPLQAGDRIYVMPWIPYRTDTLTEYASWEDYVAARHTTTYRLPNR
  VDGTGFVVYDGAVFYNKERTRNIVKYDLRTRIKSGETVINTANYHDTSPYRWGGKTDIDLAVDENGLWVI
  YATEGNNGRLVVSQLNPYTLRFEGTWETGYDKRSASNAFMVCGVLYVLRSVYVDDDSEAAGNRVDYAFNT
  NANREEPVSLTFPNPYQFISSVDYNPRDNQLYVWNNYFVVRYSLEFGPPDPSAGPATSPPLSTTTTARPT
  PLTSTASPAATTPLRRAPLTTHPVGAINQLGPDLPPATAPVPSTRRPPAPNLHVSPELFCEPREVRRVQW
  PATQQGMLVERPCPKGTRGIASFQCLPALGLWNPRGPDLSNCTSPWVNQVAQKIKSGENAANIASELARH
  TRGSIYAGDVSSSVKLMEQLLDILDAQLQALRPIERESAGKNYNKMHKRERTCKDYIKAVVETVDNLLRP
  EALESWKDMNATEQVHTATMLLDVLEEGAFLLADNVREPARFLAAKENVVLEVTVLNTEGQVQELVFPQE
  EYPRKNSIQLSAKTIKQNSRNGVVKVVFILYNNLGLFLSTENATVKLAGEAGPGGPGGASLVVNSQVIAA
  SINKESSRVFLMDPVIFTVAHLEDKNHFNANCSFWNYSERSMLGYWSTQGCRLVESNKTHTTCACSHLTN
  FAVLMAHREIYQGRINELLLSVITWVGIVISLVCLAICISTFCFLRGLQTDRNTIHKNLCINLFLAELLF
  LVGIDKTQYEIACPIFAGLLHYFFLAAFSWLCLEGVHLYLLLVEVFESEYSRTKYYYLGGYCFPALVVGI
  AAAIDYRSYGTEKACWLRVDNYFIWSFIGPVSFVIVVNLVFLMVTLHKMIRSSSVLKPDSSRLDNIKSWA
  LGAIALLFLLGLTWAFGLLFINKESVVMAYLFTTFNAFQGVFIFVFHCALQKKVHKEYSKCLRHSYCCIR
  SPPGGTHGSLKTSAMRSNTRYYTGTQSRIRRMWNDTVRKQTESSFMAGDINSTPTLNRGTMGNHLLTNPV
  LQPRGGTSPYNTLIAESVGFNPSSPPVFNSPGSYREPKHPLGGREACGMDTLPLNGNFNNSYSLRSGDFP
  PGDGGPEPPRGRNLADAAAFEKMIISELVHNNLRGSSSAAKGPPPPEPPVPPVPGGGGEEEAGGPGGADR
  AEIELLYKALEEPLLLPRAQSVLYQSDLDESESCTAEDGATSRPLSSPPGRDSLYASGANLRDSPSYPDS
  SPEGPSEALPPPPPAPPGPPEIYYTSRPPALVARNPLQGYYQVRRPSHEGYLAAPGLEGPGPDGDGQMQL
  VTSL
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001008701
• ORF Size: 4422 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001008701.1, NP_001008701.1
• RefSeq Size: 7886
• RefSeq ORF: 4425
• Locus ID: 22859
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes a member of the latrophilin subfamily of G-protein coupled receptors (GPCR). Latrophilins may function in both cell adhesion and signal transduction. In experiments with non-human species, endogenous proteolytic cleavage within a cysteine-rich GPS (G-protein-coupled-receptor proteolysis site) domain resulted in two subunits (a large extracellular N-terminal cell adhesion subunit and a subunit with substantial similarity to the secretin/calcitonin family of GPCRs) being non-covalently bound at the cell membrane. Latrophilin-1 has been shown to recruit the neurotoxin from black widow spider venom, alpha-latrotoxin, to the synapse plasma membrane. Alternative splicing results in multiple variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]