MMAA (NM_172250) Human Tagged ORF Clone

CAT#: RG223205

MMAA (GFP-tagged) - Human methylmalonic aciduria (cobalamin deficiency) cblA type (MMAA), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MMAA
• Synonyms: cblA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223205 representing NM_172250
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCATGCTGCTACCACATCCTCACCAGCATTTCCTAAAAGGCCTTTTAAGAGCACCTTTCCGATGTT
  ACCACTTCATCTTTCACTCAAGTACTCATCTCGGATCAGGAATCCCATGTGCTCAGCCGTTTAATTCTCT
  TGGACTCCATTGTACAAAGTGGATGCTGCTGTCAGATGGCTTAAAGAGAAAATTATGTGTACAAACAACC
  TTAAAGGACCACACAGAAGGACTTTCTGATAAAGAGCAAAGATTTGTGGATAAACTTTATACTGGTTTAA
  TCCAAGGGCAAAGGGCCTGTTTAGCAGAGGCCATAACTCTTGTAGAATCAACTCACAGCAGGAAAAAGGA
  GTTAGCCCAGGTGCTTCTTCAGAAAGTATTACTTTACCACAGAGAACAAGAACAATCAAATAAAGGAAAA
  CCACTAGCATTTCGAGTAGGATTGTCTGGGCCCCCTGGTGCTGGAAAATCAACATTTATAGAATATTTTG
  GAAAAATGCTTACTGAGAGAGGGCACAAATTATCTGTGCTAGCTGTGGACCCTTCTTCTTGTACTAGTGG
  TGGATCACTCTTAGGTGATAAAACCCGAATGACTGAGTTATCAAGAGATATGAATGCATACATCAGGCCA
  TCTCCTACTAGAGGAACTTTAGGAGGCGTGACAAGGACCACAAATGAAGCTATTCTGTTGTGTGAAGGAG
  CGGGATATGACATAATTCTTATTGAAACCGTTGGTGTGGGTCAGTCGGAGTTTGCTGTTGCTGACATGGT
  TGACATGTTTGTTTTACTACTGCCACCAGCAGGAGGAGATGAGCTGCAGGGTATCAAAAGGGGTATAATC
  GAGATGGCAGATCTGGTAGCTGTAACTAAATCTGATGGAGACTTGATTGTGCCAGCTCGAAGGATACAAG
  CGGAATATGTGAGTGCACTGAAATTACTCCGCAAACGTTCACAAGTCTGGAAACCAAAGGTAATTCGTAT
  TTCTGCCCGAAGTGGAGAGGGGATCTCTGAAATGTGGGATAAAATGAAAGATTTCCAGGACCTAATGCTT
  GCCAGTGGGGAGCTGACTGCCAAACGACGGAAGCAACAGAAAGTTTGGATGTGGAATCTCATTCAGGAAA
  GTGTGTTAGAGCATTTCAGGACCCACCCCACAGTCCGGGAACAGATTCCACTTCTGGAACAAAAGGTTCT
  CATTGGGGCCCTGTCCCCAGGACTAGCAGCAGACTTCTTGTTAAAAGCTTTTAAAAGCAGAGAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223205 representing NM_172250
  Red=Cloning site Green=Tags(s)
  
  MPMLLPHPHQHFLKGLLRAPFRCYHFIFHSSTHLGSGIPCAQPFNSLGLHCTKWMLLSDGLKRKLCVQTT
  LKDHTEGLSDKEQRFVDKLYTGLIQGQRACLAEAITLVESTHSRKKELAQVLLQKVLLYHREQEQSNKGK
  PLAFRVGLSGPPGAGKSTFIEYFGKMLTERGHKLSVLAVDPSSCTSGGSLLGDKTRMTELSRDMNAYIRP
  SPTRGTLGGVTRTTNEAILLCEGAGYDIILIETVGVGQSEFAVADMVDMFVLLLPPAGGDELQGIKRGII
  EMADLVAVTKSDGDLIVPARRIQAEYVSALKLLRKRSQVWKPKVIRISARSGEGISEMWDKMKDFQDLML
  ASGELTAKRRKQQKVWMWNLIQESVLEHFRTHPTVREQIPLLEQKVLIGALSPGLAADFLLKAFKSRD
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172250
• ORF Size: 1254 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_172250.1, NP_758454.1
• RefSeq Size: 1473
• RefSeq ORF: 1257
• Locus ID: 166785
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is involved in the translocation of cobalamin into the mitochondrion, where it is used in the final steps of adenosylcobalamin synthesis. Adenosylcobalamin is a coenzyme required for the activity of methylmalonyl-CoA mutase. Defects in this gene are a cause of methylmalonic aciduria. [provided by RefSeq, Jul 2008]