INDOL1 (IDO2) (NM_194294) Human Tagged ORF Clone

CAT#: RG223337

IDO2 (GFP-tagged) - Human indoleamine 2,3-dioxygenase 2 (IDO2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: IDO2
• Synonyms: INDOL1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223337 representing NM_194294
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGCATTTTCATTATTATGATACTTCAAACAAAATAATGGAGCCCCACAGACCGAATGTGAAGACAG
  CAGTGCCATTGTCTTTGGAAAGCTATCACATATCTGAAGAGTATGGCTTTCTTCTTCCAGATTCTCTGAA
  AGAACTTCCAGATCATTATAGGCCTTGGATGGAAATTGCCAACAAACTTCCTCAATTGATTGATGCTCAC
  CAGCTTCAAGCTCATGTGGACAAGATGCCCCTGCTGAGCTGCCAGTTCCTGAAGGGTCACCGGGAGCAGC
  GCCTGGCCCACCTGGTCCTGAGCTTCCTCACCATGGGTTATGTCTGGCAGGAAGGAGAGGCGCAGCCTGC
  AGAGGTCCTGCCAAGGAATCTTGCCCTTCCATTTGTCGAAGTCTCCAGGAACTTGGGGCTCCCTCCTATC
  CTGGTCCACTCAGACTTGGTGCTGACGAACTGGACCAAAAAAGATCCAGACGGATTCCTGGAAATTGGGA
  ACCTGGAGACCATCATCTCATTTCCTGGGGGAGAGAGCCTGCATGGTTTTATACTGGTGACTGCTTTGGT
  AGAGAAAGAAGCAGTGCCTGGGATAAAGGCTCTTGTTCAGGCCACGAATGCTATCTTGCAGCCCAACCAG
  GAGGCCCTGCTCCAAGCCCTGCAGCGACTGAGACTGTCTATTCAGGACATCACCAAAACCTTAGGACAGA
  TGCATGATTATGTAGATCCAGACATATTTTATGCAGGCATCCGGATCTTTCTCTCTGGATGGAAAGACAA
  CCCAGCAATGCCTGCAGGGCTGATGTATGAAGGAGTTTCCCAAGAGCCCCTGAAATACTCCGGCGGGAGT
  GCAGCTCAGAGCACAGTGCTTCATGCCTTTGATGAGTTCTTAGGCATTCGTCATAGCAAGGAAAGTGGTG
  ACTTTCTGTACAGAATGAGGGATTACATGCCTCCTTCCCATAAGGCCTTCATAGAAGACATCCACTCAGC
  ACCTTCCCTGAGGGACTACATCCTGTCATCTGGACAGGACCACTTGCTGACAGCTTATAACCAGTGTGTG
  CAGGCCCTGGCAGAGCTGCGGAGCTATCACATCACCATGGTCACCAAATACCTCATCACAGCTGCAGCCA
  AGGCAAAGCATGGGAAGCCAAACCATCTCCCAGGGCCTCCTCAGGCTTTAAAAGACAGGGGCACAGGTGG
  AACCGCAGTTATGAGCTTTCTTAAGAGTGTCAGGGATAAGACCTTGGAGTCAATCCTTCACCCACGTGGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223337 representing NM_194294
  Red=Cloning site Green=Tags(s)
  
  MLHFHYYDTSNKIMEPHRPNVKTAVPLSLESYHISEEYGFLLPDSLKELPDHYRPWMEIANKLPQLIDAH
  QLQAHVDKMPLLSCQFLKGHREQRLAHLVLSFLTMGYVWQEGEAQPAEVLPRNLALPFVEVSRNLGLPPI
  LVHSDLVLTNWTKKDPDGFLEIGNLETIISFPGGESLHGFILVTALVEKEAVPGIKALVQATNAILQPNQ
  EALLQALQRLRLSIQDITKTLGQMHDYVDPDIFYAGIRIFLSGWKDNPAMPAGLMYEGVSQEPLKYSGGS
  AAQSTVLHAFDEFLGIRHSKESGDFLYRMRDYMPPSHKAFIEDIHSAPSLRDYILSSGQDHLLTAYNQCV
  QALAELRSYHITMVTKYLITAAAKAKHGKPNHLPGPPQALKDRGTGGTAVMSFLKSVRDKTLESILHPRG
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_194294
• ORF Size: 1260 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_194294.2, NP_919270.2
• RefSeq Size: 2294
• RefSeq ORF: 1263
• Locus ID: 169355
• Protein Pathways: Metabolic pathways, Tryptophan metabolism
• Gene Summary: Along with the enzymes encoded by the INDO (MIM 147435) and TDO2 (MIM 191070) genes, the enzyme encoded by the INDOL1 gene metabolizes tryptophan in the kynurenine pathway (Ball et al., 2007 [PubMed 17499941]). [supplied by OMIM, Feb 2011]