ADAM22 (NM_016351) Human Tagged ORF Clone

CAT#: RG223610

ADAM22 (GFP-tagged) - Human ADAM metallopeptidase domain 22 (ADAM22), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ADAM22
• Synonyms: ADAM 22; EIEE61; MDC2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223610 representing NM_016351
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGCGGCAGTGGCTGTGTCCGTGCCCTTCTTGCTGCTCTGTGTCCTGGGGACCTGCCCTCCGGCGC
  GCTGCGGCCAGGCAGGAGACGCCTCATTGATGGAGCTAGAGAAGAGGAAGGAAAACCGCTTCGTGGAGCG
  CCAGAGCATCGTGCCACTGCGCCTCATCTACCGCTCGGGCGGCGAAGACGAAAGTCGGCACGACGCGCTC
  GACACGCGGGTGCGGGGCGACCTCGGTGGCCCGCAGTTGACTCATGTTGACCAAGCAAGCTTCCAGGTTG
  ATGCCTTTGGAACGTCATTCATTCTCGATGTCGTGCTAAATCATGATTTGCTGTCCTCTGAATACATAGA
  GAGACACATTGAACATGGAGGCAAGACTGTGGAAGTTAAAGGAGGAGAGCACTGTTACTACCAGGGCCAT
  ATCCGAGGAAACCCTGACTCATTTGTTGCATTGTCAACATGCCACGGACTTCATGGGATGTTCTATGACG
  GGAACCACACATATCTCATTGAGCCAGAAGAAAATGACACTACTCAAGAGGATTTCCATTTTCATTCAGT
  TTACAAATCCAGACTGTTTGAATTTTCCTTGGATGATCTTCCATCTGAATTTCAGCAAGTAAACATTACT
  CCATCAAAATTTATTTTGAAGCCAAGACCAAAAAGGAGTAAACGGCAGCTTCGTCGATATCCTCGTAATG
  TAGAAGAAGAAACCAAATACATTGAACTGATGATTGTGAATGATCACCTTATGTTTAAAAAACATCGGCT
  TTCCGTTGTACATACCAATACCTATGCGAAATCTGTGGTGAACATGGCAGATTTAATATATAAAGACCAA
  CTTAAGACCAGGATAGTATTGGTTGCTATGGAAACCTGGGCGACTGACAACAAGTTTGCCATATCTGAAA
  ATCCATTGATCACCCTACGTGAGTTTATGAAATACAGGAGGGATTTTATCAAAGAGAAAAGTGATGCAGT
  TCACCTTTTTTCGGGAAGTCAATTTGAGAGTAGCCGGAGCGGGGCAGCTTATATTGGTGGGATTTGCTCG
  TTGCTGAAAGGAGGAGGCGTGAATGAATTTGGGAAAACTGATTTAATGGCTGTTACACTTGCCCAGTCAT
  TAGCCCATAATATTGGTATTATCTCAGACAAAAGAAAGTTAGCAAGTGGTGAATGTAAATGCGAGGACAC
  GTGGTCCGGGTGCATAATGGGAGACACTGGCTATTATCTTCCTAAAAAGTTCACCCAGTGTAATATTGAA
  GAGTATCATGACTTCCTGAATAGTGGAGGTGGTGCCTGCCTTTTCAACAAACCTTCTAAGCTTCTTGATC
  CTCCTGAGTGTGGCAATGGCTTCATTGAAACTGGAGAGGAGTGTGATTGTGGAACCCCGGCCGAATGTGT
  CCTTGAAGGAGCAGAGTGTTGTAAGAAATGCACCTTGACTCAAGACTCTCAATGCAGTGACGGTCTTTGC
  TGTAAAAAGTGCAAGTTTCAGCCTATGGGCACTGTGTGCCGAGAAGCAGTAAATGATTGTGATATTCGTG
  AAACGTGCTCAGGAAATTCAAGCCAGTGTGCCCCTAATATTCATAAAATGGATGGATATTCATGTGATGG
  TGTTCAGGGAATTTGCTTTGGAGGAAGATGCAAAACCAGAGATAGACAATGCAAATACATTTGGGGGCAA
  AAGGTGACAGCATCAGACAAATATTGCTATGAGAAACTGAATATTGAAGGGACGGAGAAGGGTAACTGTG
  GGAAAGACAAAGACACATGGATACAGTGCAACAAACGGGATGTGCTTTGTGGTTACCTTTTGTGTACCAA
  TATTGGCAATATCCCAAGGCTTGGAGAACTCGATGGTGAAATCACATCTACTTTAGTTGTGCAGCAAGGA
  AGAACATTAAACTGCAGTGGTGGGCATGTTAAGCTTGAAGAAGATGTAGATCTTGGCTATGTGGAAGATG
  GGACACCTTGTGGTCCCCAAATGATGTGCTTAGAACACAGGTGTCTTCCTGTGGCTTCTTTCAACTTTAG
  TACTTGCTTGAGCAGTAAAGAAGGCACTATTTGCTCAGGAAATGGAGTTTGCAGTAATGAGCTGAAGTGT
  GTGTGTAACAGACACTGGATAGGTTCTGATTGCAACACTTACTTCCCTCACAATGATGATGCAAAGACTG
  GTATCACTCTGTCTGGCAATGGTGTTGCTGGCACCAATATCATAATAGGCATAATTGCTGGCACCATTTT
  AGTGCTGGCCCTCATATTAGGAATAACTGCGTGGGGTTATAAAAACTATCGAGAACAGAGGTCAAATGGG
  CTCTCTCATTCTTGGAGTGAAAGGATTCCAGACACAAAACATATTTCAGACATCTGTGAAAATGGGCGAC
  CTCGAAGTAACTCTTGGCAAGGTAACCTGGGAGGCAACAAAAAGAAAATCAGAGGCAAAAGATTTAGACC
  TCGGTCTAATTCAACTGAGACTTTATCTCCTGCCAAGTCTCCTTCTTCATCAACTGGGTCTATTGCCTCC
  AGCAGAAAATACCCTTACCCAATGCCTCCACTTCCTGATGAGGACAAGAAAGTGAACCGACAAAGTGCCA
  GGCTATGGGAGACATCCATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223610 representing NM_016351
  Red=Cloning site Green=Tags(s)
  
  MQAAVAVSVPFLLLCVLGTCPPARCGQAGDASLMELEKRKENRFVERQSIVPLRLIYRSGGEDESRHDAL
  DTRVRGDLGGPQLTHVDQASFQVDAFGTSFILDVVLNHDLLSSEYIERHIEHGGKTVEVKGGEHCYYQGH
  IRGNPDSFVALSTCHGLHGMFYDGNHTYLIEPEENDTTQEDFHFHSVYKSRLFEFSLDDLPSEFQQVNIT
  PSKFILKPRPKRSKRQLRRYPRNVEEETKYIELMIVNDHLMFKKHRLSVVHTNTYAKSVVNMADLIYKDQ
  LKTRIVLVAMETWATDNKFAISENPLITLREFMKYRRDFIKEKSDAVHLFSGSQFESSRSGAAYIGGICS
  LLKGGGVNEFGKTDLMAVTLAQSLAHNIGIISDKRKLASGECKCEDTWSGCIMGDTGYYLPKKFTQCNIE
  EYHDFLNSGGGACLFNKPSKLLDPPECGNGFIETGEECDCGTPAECVLEGAECCKKCTLTQDSQCSDGLC
  CKKCKFQPMGTVCREAVNDCDIRETCSGNSSQCAPNIHKMDGYSCDGVQGICFGGRCKTRDRQCKYIWGQ
  KVTASDKYCYEKLNIEGTEKGNCGKDKDTWIQCNKRDVLCGYLLCTNIGNIPRLGELDGEITSTLVVQQG
  RTLNCSGGHVKLEEDVDLGYVEDGTPCGPQMMCLEHRCLPVASFNFSTCLSSKEGTICSGNGVCSNELKC
  VCNRHWIGSDCNTYFPHNDDAKTGITLSGNGVAGTNIIIGIIAGTILVLALILGITAWGYKNYREQRSNG
  LSHSWSERIPDTKHISDICENGRPRSNSWQGNLGGNKKKIRGKRFRPRSNSTETLSPAKSPSSSTGSIAS
  SRKYPYPMPPLPDEDKKVNRQSARLWETSI
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016351
• ORF Size: 2610 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016351.3, NP_057435.2
• RefSeq Size: 3347
• RefSeq ORF: 2613
• Locus ID: 53616
• Domains: Reprolysin, DISIN, Pep_M12B_propep, ACR
• Protein Families: Druggable Genome, Protease, Transmembrane
• Gene Summary: This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. Unlike other members of the ADAM protein family, the protein encoded by this gene lacks metalloprotease activity since it has no zinc-binding motif. This gene is highly expressed in the brain and may function as an integrin ligand in the brain. In mice, it has been shown to be essential for correct myelination in the peripheral nervous system. Alternative splicing results in several transcript variants. [provided by RefSeq, Dec 2010]