anoctamin 5 (ANO5) (NM_213599) Human Tagged ORF Clone

CAT#: RG224062

ANO5 (GFP-tagged) - Human anoctamin 5 (ANO5), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ANO5
• Synonyms: GDD1; LGMD2L; LGMDR12; TMEM16E
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224062 representing NM_213599
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGACCCGGATCTCCTGGAAGTGTTGGCGGAGGAAGGGGAAAAAGTCAATAAGCATATAGACTACT
  CTTTCCAAATGAGTGAGCAGAGCCTGAGCAGCAGAGAGACCAGCTTTCTCATCAATGAAGAAACAATGCC
  TGCAAAGCGATTCAATTTGTTCCTGAGGCGGCGGCTTATGTTTCAAAAAAATCAGCAAAGCAAAGATTCT
  ATCTTCTTCCGAGATGGGATTAGGCAAATTGATTTTGTGCTTTCCTACGTTGATGACGTAAAGAAAGACG
  CAGAGTTAAAGGCGGAAAGAAGAAAAGAGTTTGAAACTAATCTCAGAAAAACAGGTCTTGAGTTGGAAAT
  AGAAGACAAAAGGGACTCGGAAGATGGAAGAACTTATTTTGTCAAGATCCATGCCCCTTGGGAGGTATTA
  GTTACCTATGCTGAAGTCTTGGGAATCAAAATGCCTATTAAGGAGAGTGATATTCCCCGCCCTAAGCACA
  CTCCTATAAGCTATGTGCTTGGACCTGTAAGACTCCCACTGAGTGTGAAGTATCCCCATCCTGAATATTT
  TACTGCACAATTCAGCAGACATCGGCAGGAGCTCTTCCTCATCGAAGATCAGGCAACCTTCTTTCCATCC
  TCATCAAGAAACAGAATTGTGTACTATATTCTCTCAAGATGTCCTTTTGGCATAGAAGATGGGAAGAAAA
  GGTTTGGGATTGAAAGACTGCTAAACTCTAACACTTACTCATCTGCCTATCCACTCCATGATGGCCAATA
  TTGGAAGCCATCAGAACCTCCCAATCCTACCAATGAAAGATACACACTTCACCAGAATTGGGCTCGATTT
  TCCTATTTCTACAAGGAGCAGCCTTTAGACTTGATTAAGAATTATTATGGAGAAAAAATTGGTATCTATT
  TTGTCTTTCTTGGATTTTACACAGAAATGCTATTCTTTGCAGCTGTAGTTGGCTTAGCTTGTTTTATTTA
  TGGCTTATTATCAATGGAACATAACACAAGCAGCACTGAAATCTGTGACCCTGAGATTGGTGGTCAGATG
  ATCATGTGCCCACTCTGTGATCAAGTGTGTGATTATTGGAGACTAAATAGTACGTGTTTGGCTTCAAAGT
  TCTCCCATTTGTTTGATAATGAGTCAACAGTGTTCTTTGCAATATTCATGGGAATTTGGGTCACCTTATT
  TTTGGAGTTTTGGAAACAACGACAAGCCAGACTGGAATATGAATGGGACCTGGTGGACTTTGAAGAGGAA
  CAGCAGCAGCTTCAGCTGAGACCAGAATTTGAAGCTATGTGTAAACACAGGAAATTGAATGCAGTGACTA
  AGGAGATGGAACCTTACATGCCTCTATACACGCGTATTCCATGGTACTTTCTTTCAGGAGCCACAGTGAC
  ATTATGGATGTCTCTTGTCGTCACCAGTATGGTAGCTGTAATTGTGTACCGCCTGTCAGTCTTTGCTACA
  TTTGCTAGTTTCATGGAAAGTGATGCATCCTTAAAGCAGGTCAAAAGCTTCCTTACTCCTCAGATAACCA
  CATCACTCACAGGATCATGCTTGAACTTTATTGTCATCTTGATCTTGAATTTCTTTTATGAAAAGATATC
  TGCCTGGATCACAAAAATGGAAATTCCTCGAACATACCAGGAGTATGAGAGCAGTCTTACCTTGAAAATG
  TTCCTGTTTCAGTTTGTAAATTTTTACTCATCCTGCTTCTACGTAGCTTTCTTTAAAGGGAAGTTCGTAG
  GCTATCCTGGAAAATACACATATTTATTTAATGAGTGGAGAAGTGAAGAGTGTGATCCTGGAGGCTGTCT
  TATAGAATTGACAACCCAATTGACCATTATAATGACCGGGAAACAGATTTTTGGAAACATTAAAGAAGCC
  ATTTATCCCTTGGCTTTGAATTGGTGGAGACGCCGAAAAGCTCGGACAAACTCTGAGAAGCTGTATAGTC
  GATGGGAGCAGGATCATGACCTTGAAAGTTTTGGACCCCTTGGGCTTTTCTATGAGTACTTAGAAACAGT
  TACTCAATTTGGATTTGTTACACTATTTGTGGCCTCTTTTCCTTTGGCTCCTCTTCTTGCTCTCATAAAT
  AATATTGTAGAGATTCGAGTGGATGCCTGGAAACTTACCACTCAATACAGGAGAACTGTAGCTTCTAAAG
  CTCATAGCATAGGTGTTTGGCAAGACATTCTTTATGGAATGGCTGTCCTTTCTGTTGCAACTAATGCCTT
  TATTGTTGCATTTACGTCAGACATCATTCCCCGTCTAGTTTACTACTATGCTTACTCAACAAATGCCACA
  CAGCCTATGACAGGATATGTGAATAATAGCCTGTCAGTATTCCTGATAGCTGATTTTCCAAACCACACTG
  CACCTTCGGAAAAACGAGACTTCATCACTTGCAGGTACAGAGATTACAGATATCCTCCTGATGACGAGAA
  TAAATATTTTCATAATATGCAATTCTGGCATGTCCTTGCTGCCAAGATGACCTTCATCATTGTTATGGAA
  CATGTTGTGTTTTTAGTTAAATTTTTGCTGGCCTGGATGATACCTGATGTTCCAAAAGATGTTGTGGAGA
  GAATCAAGAGAGAAAAGTTAATGACTATCAAGATTCTCCATGATTTTGAGCTCAACAAATTAAAAGAGAA
  CTTGGGAATTAATTCTAATGAATTTGCCAAGCATGTCATGATTGAGGAAAACAAAGCACAGCTGGCTAAA
  TCAACACTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224062 representing NM_213599
  Red=Cloning site Green=Tags(s)
  
  MGDPDLLEVLAEEGEKVNKHIDYSFQMSEQSLSSRETSFLINEETMPAKRFNLFLRRRLMFQKNQQSKDS
  IFFRDGIRQIDFVLSYVDDVKKDAELKAERRKEFETNLRKTGLELEIEDKRDSEDGRTYFVKIHAPWEVL
  VTYAEVLGIKMPIKESDIPRPKHTPISYVLGPVRLPLSVKYPHPEYFTAQFSRHRQELFLIEDQATFFPS
  SSRNRIVYYILSRCPFGIEDGKKRFGIERLLNSNTYSSAYPLHDGQYWKPSEPPNPTNERYTLHQNWARF
  SYFYKEQPLDLIKNYYGEKIGIYFVFLGFYTEMLFFAAVVGLACFIYGLLSMEHNTSSTEICDPEIGGQM
  IMCPLCDQVCDYWRLNSTCLASKFSHLFDNESTVFFAIFMGIWVTLFLEFWKQRQARLEYEWDLVDFEEE
  QQQLQLRPEFEAMCKHRKLNAVTKEMEPYMPLYTRIPWYFLSGATVTLWMSLVVTSMVAVIVYRLSVFAT
  FASFMESDASLKQVKSFLTPQITTSLTGSCLNFIVILILNFFYEKISAWITKMEIPRTYQEYESSLTLKM
  FLFQFVNFYSSCFYVAFFKGKFVGYPGKYTYLFNEWRSEECDPGGCLIELTTQLTIIMTGKQIFGNIKEA
  IYPLALNWWRRRKARTNSEKLYSRWEQDHDLESFGPLGLFYEYLETVTQFGFVTLFVASFPLAPLLALIN
  NIVEIRVDAWKLTTQYRRTVASKAHSIGVWQDILYGMAVLSVATNAFIVAFTSDIIPRLVYYYAYSTNAT
  QPMTGYVNNSLSVFLIADFPNHTAPSEKRDFITCRYRDYRYPPDDENKYFHNMQFWHVLAAKMTFIIVME
  HVVFLVKFLLAWMIPDVPKDVVERIKREKLMTIKILHDFELNKLKENLGINSNEFAKHVMIEENKAQLAK
  STL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_213599
• ORF Size: 2739 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_213599.1, NP_998764.1
• RefSeq Size: 3147
• RefSeq ORF: 2742
• Locus ID: 203859
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of the anoctamin family of transmembrane proteins. The encoded protein is likely a calcium activated chloride channel. Mutations in this gene have been associated with gnathodiaphyseal dysplasia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]