ME3 (NM_006680) Human Tagged ORF Clone

CAT#: RG224151

ME3 (GFP-tagged) - Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ME3
• Synonyms: NADP-ME
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224151 representing NM_006680
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTGCCGCGCTGGGGACAGGCACGCGGCTGGCTCCCTGGCCGGGCCGGGCCTGCGGCGCCCTCCCGC
  GCTGGACACCCACCGCGCCCGCCCAAGGCTGCCACTCCAAGCCTGGCCCGGCGCGCCCTGTGCCCCTGAA
  GAAGCGCGGATACGATGTCACCAGGAACCCTCATCTCAACAAGGGGATGGCCTTTACCCTTGAAGAAAGG
  CTGCAGCTTGGAATCCACGGCCTAATCCCGCCCTGCTTTCTGAGCCAGGACGTCCAGCTCCTCCGAATCA
  TGAGATATTACGAGCGGCAGCAGAGTGACCTGGACAAGTACATCATTCTCATGACACTCCAAGACCGGAA
  CGAGAAGCTCTTCTACCGAGTGCTGACTTCGGACGTGGAGAAGTTCATGCCAATCGTGTACACGCCTACC
  GTGGGGCTGGCCTGTCAGCACTATGGCCTGACTTTCCGCAGGCCCCGTGGACTGTTCATCACCATTCATG
  ACAAAGGTCATCTTGCAACAATGCTGAATTCTTGGCCAGAAGACAATATTAAGGCCGTGGTGGTGACTGA
  TGGGGAGCGCATCCTGGGCCTGGGAGACCTGGGCTGCTACGGCATGGGCATCCCTGTGGGCAAGCTGGCC
  CTGTACACGGCATGCGGAGGGGTGAACCCGCAGCAGTGCCTCCCTGTGCTGCTGGACGTCGGCACCAACA
  ATGAGGAGCTGCTCAGAGACCCTCTGTACATCGGCCTGAAACACCAGCGCGTGCACGGGAAGGCATACGA
  TGACTTGCTGGATGAGTTCATGCAGGCTGTGACAGACAAGTTTGGAATAAATTGCCTCATCCAATTTGAA
  GACTTCGCCAATGCCAATGCCTTCCGCCTGCTCAACAAATACCGTAACAAGTACTGCATGTTCAATGATG
  ACATCCAAGGCACAGCCTCCGTTGCTGTGGCAGGGATCTTGGCTGCTCTGCGAATCACCAAGAACAAGCT
  TTCCAATCACGTGTTTGTTTTCCAAGGTGCAGGCGAGGCAGCTATGGGCATTGCCCACCTCCTTGTCATG
  GCCCTAGAGAAAGAAGGTGTACCGAAGGCAGAGGCCACAAGAAAGATCTGGATGGTGGACTCTAAAGGGC
  TCATTGTCAAGGGGAGGAGCCACCTGAACCATGAAAAGGAGATGTTTGCCCAAGACCATCCTGAAGTCAA
  CTCCCTGGAGGAGGTGGTGAGGCTGGTGAAGCCCACAGCCATCATAGGTGTTGCTGCCATCGCAGGAGCC
  TTCACGGAGCAGATTCTGAGGGACATGGCCTCCTTCCACGAGCGCCCTATCATCTTTGCCCTGAGCAACC
  CCACCAGCAAGGCCGAGTGCACGGCTGAGAAGTGCTACCGGGTCACCGAGGGCCGAGGGATTTTTGCCAG
  TGGAAGTCCTTTTAAGAGTGTGACTCTGGAAGATGGCAAGACCTTCATTCCTGGGCAGGGAAACAATGCT
  TACGTGTTCCCCGGGGTGGCACTGGGAGTCATCGCCGGCGGGATCCGGCACATCCCAGATGAGATCTTCC
  TCCTGACAGCAGAGCAAATTGCCCAGGAAGTCTCTGAGCAGCATCTGTCCCAGGGGAGACTCTACCCACC
  ACTCAGCACCATCCGAGACGTGTCTTTGAGAATTGCCATCAAAGTTCTCGACTACGCGTACAAACACAAC
  CTGGCTTCCTACTACCCAGAGCCTAAGGACAAGGAGGCTTTTGTAAGATCCCTGGTCTACACTCCAGACT
  ATGACTCCTTTACACTGGACAGCTACACTTGGCCCAAGGAAGCCATGAATGTTCAGACGGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006680
• ORF Size: 1812 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_006680.2, NP_006671.2
• RefSeq Size: 2090
• RefSeq ORF: 1815
• Locus ID: 10873
• Domains: malic
• Protein Pathways: Metabolic pathways, Pyruvate metabolism
• Gene Summary: Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]