ME3 (NM_006680) Human Untagged Clone

CAT#: SC115951

ME3 (untagged)-Human malic enzyme 3, NADP(+)-dependent, mitochondrial (ME3), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ME3
• Synonyms: NADP-ME
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115951 sequence for NM_006680 edited (data generated by NextGen Sequencing)
  ATGGGTGCCGCGCTGGGGACAGGCACGCGGCTGGCTCCCTGGCCGGGCCGGGCCTGCGGC
  GCCCTCCCGCGCTGGACACCCACCGCGCCCGCCCAAGGCTGCCACTCCAAGCCTGGCCCG
  GCGCGCCCTGTGCCCCTGAAGAAGCGCGGATACGATGTCACCAGGAACCCTCATCTCAAC
  AAGGGGATGGCCTTTACCCTTGAAGAAAGGCTGCAGCTTGGAATCCACGGCCTAATCCCG
  CCCTGCTTTCTGAGCCAGGACGTCCAGCTCCTCCGAATCATGAGATATTACGAGCGGCAG
  CAGAGTGACCTGGACAAGTACATCATTCTCATGACACTCCAAGACCGGAACGAGAAGCTC
  TTCTACCGAGTGCTGACTTCGGACGTGGAGAAGTTCATGCCAATCGTGTACACGCCTACC
  GTGGGGCTGGCCTGTCAGCACTATGGCCTGACTTTCCGCAGGCCCCGTGGACTGTTCATC
  ACCATTCATGACAAAGGTCATCTTGCAACAATGCTGAATTCTTGGCCAGAAGACAATATT
  AAGGCCGTGGTGGTGACTGATGGGGAGCGCATCCTGGGCCTGGGAGACCTGGGCTGCTAC
  GGCATGGGCATCCCTGTGGGCAAGCTGGCCCTGTACACGGCATGCGGAGGGGTGAACCCG
  CAGCAGTGCCTCCCTGTGCTGCTGGACGTCGGCACCAACAATGAGGAGCTGCTCAGAGAC
  CCTCTGTACATCGGCCTGAAACACCAGCGCGTGCACGGGAAGGCATACGATGACTTGCTG
  GATGAGTTCATGCAGGCTGTGACAGACAAGTTTGGAATAAATTGCCTCATCCAATTTGAA
  GACTTCGCCAATGCCAATGCCTTCCGCCTGCTCAACAAATACCGTAACAAGTACTGCATG
  TTCAATGATGACATCCAAGGCACAGCCTCCGTTGCTGTGGCAGGGATCTTGGCTGCTCTG
  CGAATCACCAACAACAAGCTTTCCAATCACGTGTTTGTTTTCCAAGGTGCAGGCGAGGCA
  GCTATGGGCATTGCCCACCTCCTTGTCATGGCCCTAGAGAAAGAAGGTGTACCGAAGGCA
  GAGGCCACAAGAAAGATCTGGATGGTGGACTCTAAAGGGCTCATTGTCAAGGGGAGGAGC
  CACCTGAACCATGAAAAGGAGATGTTTGCCCAAGACCATCCTGAAGTCAACTCCCTGGAG
  GAGGTGGTGAGGCTGGTGAAGCCCACAGCCATCATAGGTGTTGCTGCCATCGCAGGAGCC
  TTCACGGAGCAGATTCTGAGGGACATGGCCTCCTTCCACGAGCGCCCTATCATCTTTGCC
  CTGAGCAACCCCACCAGCAAGGCCGAGTGCACGGCTGAGAAGTGCTACCGGGTCACCGAG
  GGCCGAGGGATTTTTGCCAGTGGAAGTCCTTTTAAGAGTGTGACTCTGGAAGATGGCAAG
  ACCTTCATTCCTGGGCAGGGAAACAATGCTTACGTGTTCCCCGGGGTGGCACTGGGAGTC
  ATCGCCGGCGGGATCCGGCACATCCCAGATGAGATCTTCCTCCTGACAGCAGAGCAAATT
  GCCCAGGAAGTCTCTGAGCAGCATCTGTCCCAGGGGAGACTCTATCCACCACTCAGCACC
  ATCCGAGACGTGTCTTTGAGAATTGCCATCAAAGTTCTCGACTACGCGTACAAACACAAC
  CTGGCTTCCTACTACCCAGAGCCTAAGGACAAGGAGGCTTTTGTAAGATCCCTGGTCTAC
  ACTCCAGACTATGACTCCTTTACACTGGACAGCTACACTTGGCCCAAGGAAGCCATGAAT
  GTTCAGACGGTCTGA
  
  Clone variation with respect to NM_006680.2
  972 g=>c;1605 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006680 unedited
  GGGTCAATATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTCAGGCG
  GGCATTCTGAGCTGCTTCTTGTCACTGGGNGAAAACCACGTGCACGCAGTGCTGAGGCGC
  CCCACGCGCGTCCTCAGGGGAAGCCAGGCACTGAGAACAAAGGTTGTGGGACCGCTCTTT
  GAGGAACTCACCAGCTCCGCGAGCTCGCGGCCGCCAGCGCGGTCCACCCATTGGCGGGAG
  AGGACTCACAGACCGCAGCTCCTCACCCCGCCTGCTCCGCAGCGGGACCGCGTAGACCTC
  AGCTCCCCGAGCAGCGCAGGTGGGCCAGGGAAGGAGAGGACCGAGGTCTGCCAAGGACCA
  TGGGTGCCGCGCTGGGGACAGGCACGCGGCTGGCTCCCTGGCCGGGCCGGGCCTGCGGCG
  CCCTCCCGCGCTGGACACCCACCGCGCCCGCCCAAGGCTGCCACTCCAAGCCTGGCCCGG
  CGCGCCCTGTGCCCCTGAAGAAGCGCGGATACGATGTCACCAGGAACCCTCATCTCAACA
  AGGGGATGGCCTTTACCCTTGAAGAAAGGCTGCAGCTTGGAATCCACGGCCTAATCCCGC
  CCTGCTTTCTGAGCCAGGACGTCCAGCTCCTCCGAATCATGAGATATTACGAGCGGCAGC
  AGAGTGACCTGGACAAGTACATCATTCTCATGACACTCCAAGACCGGAACGAGAAGCTCT
  TCTACCGAGTGCTGACTTCGGACGTGGAGAAGTTCATGCCAATCGTGTACACGCCTACCG
  TGGGGCTGGCCTGTCAGCACTATGGCCTGACTTTCCGCAGGCCCCGTGGACTGTTCATCA
  CCATTCATGACANAGGTCATCTTTGCACAATGCTGAATCTTGGCCAGAAGACATATTAGG
  CA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006680 unedited
  GTACGCGGCCGCTTTCTANATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTCAAAAACAAG
  GCTTTTATTCACTGTATGCCTTGGCATCTCCTCTCACACCAAAAAGTTTAAAAGAAAAAA
  CACAGCGACAAGGATTCACTTGGGCCATTTTGGAACCCATTTATATTGGGGGTGTTACTC
  TGGGCTTCATCTAGCCCCATACTAGCCTCTGAAACTGCCTCAAACCGTCTGAACATTCAT
  GGCTTCCTTGGGCCAAGTGTAGCTGTCCAGTGTAAAGGAGTCATAGTCTGGAGTGTAAAC
  CAGGGATCTTACAAAAGCCTCCTTGTCCTTAGGCTCTGGGTAGTAGGAAGCCAGGTTGTG
  TTTGTACGCGTAGTCGAAAACTTTGATGGCAATTCTCAAAGACACGTCTCGGATGGTGCT
  GAGTGGTGGATAAAGTCTCCCCTGGGACAGATGCTGCTCAAAGACTTCCTGGGCAATTTG
  CTCTGCTGTCAGGAGGAAAATCTCATCTGGGATGTGCCGGATCCCGCCGGCGATGACTCC
  CAGTGCCACCCCGGGGAACACGCAAGCATTGTTTCCCTGCCCAGGAATGAAGGTCTTGCC
  ATCTTCCAAAGCCACACTCTTAAAAGGACTTCCACTGGAAAAAATCCTTCGGTCCTTCGG
  AGACCCGGAAGAACTTATAAACCGCGCACTTCGGCCTTGCTGGGGGGGTTCTCCAGGGCA
  AAAATAATAGGCCGCTCCGGGAAGGAGGCCCTGTCCCTCAAAATCTGCTCCGTGAAAGCC
  TCCTGCTATGGCAGACACACCTATGTAGGGCTGTGGGCTTACACAGCCCTACCCACTTCT
  CCAGGGAGTCTATTTAAGAATGGCCTTGGCACAACTTTCCTTTTATGGTCCAGGGGGTTC
  CCCCTCACAAAGACACCTCTAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006680
• ORF Size: 1815 bp
• Insert Size: 2260
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_006680.2, NP_006671.2
• RefSeq Size: 2090
• RefSeq ORF: 1815
• Locus ID: 10873
• Domains: malic
• Protein Pathways: Metabolic pathways, Pyruvate metabolism
• Gene Summary: Malic enzyme catalyzes the oxidative decarboxylation of malate to pyruvate using either NAD+ or NADP+ as a cofactor. Mammalian tissues contain 3 distinct isoforms of malic enzyme: a cytosolic NADP(+)-dependent isoform, a mitochondrial NADP(+)-dependent isoform, and a mitochondrial NAD(+)-dependent isoform. This gene encodes a mitochondrial NADP(+)-dependent isoform. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) and variants 2-4 all encode the same protein.