UBE1C (NM_198197) Human Tagged ORF Clone

CAT#: RG224294

UBE1C (GFP-tagged) - Human ubiquitin-activating enzyme E1C (UBA3 homolog, yeast) (UBE1C), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: UBE1C
• Synonyms: UBA3, hUba3, MGC22384, DKFZp566J164
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224294 representing NM_198197
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACTATAGATGTTTCCAATCTAAATAGGCAGTTTTTATTTAGGCCTAAAGATATTGGAAGACCTA
  AGGCTGAAGTTGCTGCAGAATTTCTAAATGACAGAGTTCCTAATTGCAATGTAGTTCCACATTTCAACAA
  GATTCAAGATTTTAACGACACTTTCTATCGACAATTTCATATTATTGTATGTGGACTGGACTCTATCATC
  GCCAGAAGATGGATAAATGGCATGCTGATATCTCTTCTAAATTATGAAGATGGTGTCTTAGATCCAAGCT
  CCATTGTCCCTTTGATAGATGGGGGGACAGAAGGTTTTAAAGGAAATGCCCGGGTGATTCTGCCTGGAAT
  GACTGCTTGTATCGAATGCACGCTGGAACTTTATCCACCACAGGTTAATTTTCCCATGTGCACCATTGCA
  TCTATGCCCAGGCTACCAGAACACTGTATTGAGTATGTAAGGATGTTGCAGTGGCCTAAGGAGCAGCCTT
  TTGGAGAAGGGGTTCCATTAGATGGAGATGATCCTGAACATATACAATGGATTTTCCAAAAATCCCTAGA
  GAGAGCATCACAATATAATATTAGGGGTGTTACGTATAGGCTCACTCAAGGGGTAGTAAAAAGAATCATT
  CCTGCAGTAGCTTCCACAAATGCAGTCATTGCAGCTGTGTGTGCCACTGAGGTTTTTAAAATAGCCACAA
  GTGCATACATTCCCTTGAATAATTACTTGGTGTTTAATGATGTAGATGGGCTGTATACATACACATTTGA
  AGCAGAAAGAAAGGAAAACTGCCCAGCTTGTAGCCAGCTTCCTCAAAATATTCAGTTTTCTCCATCAGCT
  AAACTACAGGAGGTTTTGGATTATCTAACCAATAGTGCTTCTCTGCAAATGAAATCTCCAGCCATCACAG
  CCACCCTAGAGGGAAAAAATAGAACACTTTACTTACAGTCGGTAACCTCTATTGAAGAACGAACAAGGCC
  AAATCTCTCCAAAACATTGAAAGAATTGGGGCTTGTTGATGGACAAGAACTGGCGGTTGCTGATGTCACC
  ACCCCACAGACTGTACTATTCAAACTTCATTTTACTTCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224294 representing NM_198197
  Red=Cloning site Green=Tags(s)
  
  MDTIDVSNLNRQFLFRPKDIGRPKAEVAAEFLNDRVPNCNVVPHFNKIQDFNDTFYRQFHIIVCGLDSII
  ARRWINGMLISLLNYEDGVLDPSSIVPLIDGGTEGFKGNARVILPGMTACIECTLELYPPQVNFPMCTIA
  SMPRLPEHCIEYVRMLQWPKEQPFGEGVPLDGDDPEHIQWIFQKSLERASQYNIRGVTYRLTQGVVKRII
  PAVASTNAVIAAVCATEVFKIATSAYIPLNNYLVFNDVDGLYTYTFEAERKENCPACSQLPQNIQFSPSA
  KLQEVLDYLTNSASLQMKSPAITATLEGKNRTLYLQSVTSIEERTRPNLSKTLKELGLVDGQELAVADVT
  TPQTVLFKLHFTS
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198197
• ORF Size: 1089 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_198197.1, NP_937840.1
• RefSeq Size: 2015
• RefSeq ORF: 1091
• Locus ID: 9039
• Protein Pathways: Ubiquitin mediated proteolysis
• Gene Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]