EED (NM_152991) Human Tagged ORF Clone

CAT#: RG224366

EED (GFP-tagged) - Human embryonic ectoderm development (EED), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EED
• Synonyms: COGIS; HEED; WAIT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224366 representing NM_152991
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGAGAGGGAAGTGTCGACTGCGCCGGCGGGAACAGACATGCCTGCGGCCAAGAAGCAGAAGCTGA
  GCAGTGACGAGAACAGCAATCCAGACCTCTCTGGAGACGAGAATGATGACGCTGTCAGTATAGAAAGTGG
  TACAAACACTGAACGCCCTGATACACCTACAAACACGCCAAATGCACCTGGAAGGAAAAGTTGGGGAAAG
  GGAAAATGGAAGTCAAAGAAATGCAAATATTCTTTCAAATGTGTAAATAGTCTCAAGGAAGATCATAACC
  AACCATTGTTTGGAGTTCAGTTTAACTGGCACAGTAAAGAAGGAGATCCATTAGTGTTTGCAACTGTAGG
  AAGCAACAGAGTTACCTTGTATGAATGTCATTCACAAGGAGAAATCCGGTTGTTGCAATCTTACGTGGAT
  GCTGATGCTGATGAAAACTTTTACACTTGTGCATGGACCTATGATAGCAATACGAGCCATCCTCTGCTGG
  CTGTAGCTGGATCTAGAGGCATAATTAGGATAATAAATCCTATAACAATGCAGTGTATAAAGCACTATGT
  TGGCCATGGAAATGCTATCAATGAGCTGAAATTCCATCCAAGAGATCCAAATCTTCTCCTGTCAGTAAGT
  AAAGATCATGCTTTACGATTATGGAATATCCAGACGGACACTCTGGTGGCAATATTTGGAGGCGTAGAAG
  GGCACAGAGATGAAGTTCTAAGTGCTGATTATGATCTTTTGGGTGAAAAAATAATGTCCTGTGGTATGGA
  TCATTCTCTTAAACTTTGGAGGATCAATTCAAAGAGAATGATGAATGCAATTAAGGAATCTTATGATTAT
  AATCCAAATAAAACTAACAGGCCATTTATTTCTCAGAAAATCCATTTTCCTGATTTTTCTACCAGAGACA
  TACATAGGAATTATGTTGATTGTGTGCGATGGTTAGGCGATTTGATACTTTCTAAGTCTTGTGAAAATGC
  CATTGTGTGCTGGAAACCTGGCAAGATGGAAGATGATATAGATAAAATTAAACCCAGTGAATCTAATGTG
  ACTATTCTTGGGCGATTTGATTACAGCCAGTGTGACATTTGGTACATGAGGTTTTCTATGGATTTCTGGC
  AAAAGATGCTTGCATTGGGCAATCAAGTTGGCAAACTTTATGTTTGGGATTTAGAAGTAGAAGATCCTCA
  TAAAGCCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224366 representing NM_152991
  Red=Cloning site Green=Tags(s)
  
  MSEREVSTAPAGTDMPAAKKQKLSSDENSNPDLSGDENDDAVSIESGTNTERPDTPTNTPNAPGRKSWGK
  GKWKSKKCKYSFKCVNSLKEDHNQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVD
  ADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVS
  KDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMMNAIKESYDY
  NPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNV
  TILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEVEDPHKAK
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152991
• ORF Size: 1200 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_152991.1, NP_694536.1
• RefSeq Size: 2413
• RefSeq ORF: 1203
• Locus ID: 8726
• Domains: WD40
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]