TAF6 (NM_139123) Human Tagged ORF Clone

CAT#: RG224515

TAF6 (GFP-tagged) - Human TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TAF6
• Synonyms: DKFZp781E21155; MGC:8964; TAF(II)80; TAF2E; TAFII70; TAFII80; TAFII85
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224515 representing NM_139123
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAGGAGAAGAAGCTGAAGCTTAGCAACACTGTGCTGCCCTCGGAGTCCATGAAGGTGGTGGCTG
  AATCCATGGGCATCGCCCAGATTCAGGAGGAGACCTGCCAGCTGCTAACGGATGAGGATGCCTTGAAGTT
  CATGCACATGGGGAAGCGGCAGAAGCTCACCACCAGTGACATTGACTACGCCTTGAAGCTAAAGAATGTC
  GAGCCACTCTATGGCTTCCACGCCCAGGAGTTCATTCCTTTCCGCTTCGCCTCTGGTGGGGGCCGGGAGC
  TTTACTTCTATGAGGAGAAGGAGGTTGATCTGAGCGACATCATCAATACCCCTCTGCCCCGGGTGCCCCT
  GGACGTCTGCCTCAAAGCTCATTGGCTGAGCATCGAGGGCTGCCAGCCAGCTATCCCCGAGAACCCGCCC
  CCAGCTCCCAAAGAGCAACAGAAGGCTGAAGCCACAGAACCCCTGAAGTCAGCCAAGCCAGGCCAGGAGG
  AAGACGGACCCCTGAAGGGCAAAGGTCAAGGGGCCACCACAGCCGACGGCAAAGGGAAAGAGAAGAAGGC
  GCCGCCCTTGCTGGAGGGGGCCCCCTTGCGACTGAAGCCCCGGAGCATCCACGAGTTGTCTGTGGAGCAG
  CAGCTCTACTACAAGGAGATCACCGAGGCCTGCGTGGGCTCCTGCGAGGCCAAGAGGGCGGAAGCCCTGC
  AAAGCATTGCCACGGACCCTGGACTGTATCAGATGCTGCCACGGTTCAGTACCTTTATCTCGGAGGGGGT
  CCGTGTGAACGTGGTTCAGAACAACCTGGCCCTACTCATCTACCTGATGCGTATGGTGAAAGCGCTGATG
  GACAACCCCACGCTCTATCTAGAAAAATACGTCCATGAGCTGATTCCAGCTGTGATGACCTGCATCGTGA
  GCAGACAGTTGTGCCTGCGACCAGATGTGGACAATCACTGGGCACTCCGAGACTTTGCTGCCCGCCTGGT
  GGCCCAGATCTGCAAGCATTTTAGCACAACCACTAACAACATCCAGTCCCGGATCACCAAGACCTTCACC
  AAGAGCTGGGTGGACGAGAAGACGCCCTGGACGACTCGTTATGGCTCCATCGCAGGCTTGGCTGAGCTGG
  GACACGATGTTATCAAGACTCTGATTCTGCCCCGGCTGCAGCAGGAAGGGGAGCGGATCCGCAGTGTGCT
  GGACGGCCCTGTGCTGAGCAACATTGACCGGATTGGAGCAGACCATGTGCAGAGCCTCCTGCTGAAACAC
  TGTGCTCCTGTTCTGGCAAAGCTGCGCCCACCGCCTGACAATCAGGACGCCTATCGGGCAGAATTCGGGT
  CCCTTGGGCCCCTCCTCTGCTCCCAGGTGGTCAAGGCTCGGGCCCAGGCTGCTCTGCAGGCTCAGCAGGT
  CAACAGGACCACTCTGACCATCACGCAGCCCCGGCCCACGCTGACCCTCTCGCAGGCCCCACAGCCTGGC
  CCTCGCACCCCTGGCTTGCTGAAGGTTCCTGGCTCCATCGCACTTCCTGTCCAGACACTGGTGTCTGCAC
  GAGCGGCTGCCCCACCACAGCCTTCCCCTCCTCCAACCAAGTTTATTGTAATGTCATCGTCCTCCAGCGC
  CCCATCCACCCAGCAGGTCCTGTCCCTCAGCACCTCGGCCCCCGGCTCAGGTTCCACCACCACTTCGCCC
  GTCACCACCACCGTCCCCAGCGTGCAGCCCATCGTCAAGTTGGTCTCCACCGCCACCACCGCACCCCCCA
  GCACTGCTCCCTCTGGTCCTGGGAGTGTCCAGAAGTACATCGTGGTCTCACTTCCCCCAACAGGGGAGGG
  CAAAGGAGGCCCCACCTCCCATCCTTCTCCAGTTCCTCCCCCGGCATCGTCCCCGTCCCCACTCAGCGGC
  AGTGCCCTTTGTGGGGGGAAGCAGGAGGCTGGGGACAGTCCCCCTCCAGCTCCAGGGACTCCAAAAGCCA
  ATGGCTCCCAGCCCAACTCCGGCTCCCCTCAGCCTGCTCCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224515 representing NM_139123
  Red=Cloning site Green=Tags(s)
  
  MAEEKKLKLSNTVLPSESMKVVAESMGIAQIQEETCQLLTDEDALKFMHMGKRQKLTTSDIDYALKLKNV
  EPLYGFHAQEFIPFRFASGGGRELYFYEEKEVDLSDIINTPLPRVPLDVCLKAHWLSIEGCQPAIPENPP
  PAPKEQQKAEATEPLKSAKPGQEEDGPLKGKGQGATTADGKGKEKKAPPLLEGAPLRLKPRSIHELSVEQ
  QLYYKEITEACVGSCEAKRAEALQSIATDPGLYQMLPRFSTFISEGVRVNVVQNNLALLIYLMRMVKALM
  DNPTLYLEKYVHELIPAVMTCIVSRQLCLRPDVDNHWALRDFAARLVAQICKHFSTTTNNIQSRITKTFT
  KSWVDEKTPWTTRYGSIAGLAELGHDVIKTLILPRLQQEGERIRSVLDGPVLSNIDRIGADHVQSLLLKH
  CAPVLAKLRPPPDNQDAYRAEFGSLGPLLCSQVVKARAQAALQAQQVNRTTLTITQPRPTLTLSQAPQPG
  PRTPGLLKVPGSIALPVQTLVSARAAAPPQPSPPPTKFIVMSSSSSAPSTQQVLSLSTSAPGSGSTTTSP
  VTTTVPSVQPIVKLVSTATTAPPSTAPSGPGSVQKYIVVSLPPTGEGKGGPTSHPSPVPPPASSPSPLSG
  SALCGGKQEAGDSPPPAPGTPKANGSQPNSGSPQPAP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_139123
• ORF Size: 2001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_139123.1, NP_620835.1
• RefSeq Size: 2707 bp
• RefSeq ORF: 2003 bp
• Locus ID: 6878
• Cytogenetics: 7q22.1
• Domains: TAF
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Gene Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]'