PFKFB1 (NM_002625) Human Tagged ORF Clone

CAT#: RG224599

PFKFB1 (GFP-tagged) - Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: PFKFB1
• Synonyms: F6PK; HL2K; PFRX
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG224599 representing NM_002625
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCCAGAGATGGGAGAGCTCACCCAAACCAGGTTGCAGAAGATCTGGATTCCACACAGCAGCGGCA
  GCAGCAGGCTGCAACGGAGAAGGGGCTCATCCATACCCCAGTTTACCAATTCCCCCACAATGGTGATCAT
  GGTGGGTTTACCAGCTCGAGGCAAGACCTATATCTCCACAAAGCTCACACGATATCTCAACTGGATAGGA
  ACACCAACTAAAGTGTTTAATTTAGGCCAGTATCGACGAGAGGCAGTGAGCTACAAGAACTATGAATTCT
  TTCTTCCAGACAACATGGAAGCCCTGCAAATCAGGAAGCAGTGCGCCCTGGCAGCCCTGAAGGATGTTCA
  CAACTATCTCAGCCATGAGGAAGGTCATGTTGCGGTTTTTGATGCCACCAACACTACCAGAGAACGACGG
  TCACTGATCCTGCAGTTTGCAAAAGAACATGGTTACAAGGTGTTTTTCATTGAGTCCATTTGTAATGACC
  CTGGCATAATTGCAGAAAACATCAGGCAAGTGAAACTTGGCAGCCCTGATTATATAGACTGTGACCGGGA
  AAAGGTTCTGGAAGACTTTCTAAAGAGAATTGAGTGCTATGAGGTCAACTACCAACCCTTGGATGAGGAA
  CTGGACAGCCACCTGTCCTACATCAAGATCTTCGACGTGGGCACACGCTACATGGTGAACCGAGTGCAGG
  ATCACATCCAGAGCCGCACAGTCTACTACCTCATGAATATCCATGTCACACCTCGCTCCATCTACCTTTG
  CCGACATGGCGAGAGTGAACTCAACATCAGAGGCCGCATCGGAGGTGACTCTGGCCTCTCAGTTCGCGGC
  AAGCAGTATGCCTATGCCCTGGCCAACTTCATTCAGTCCCAGGGCATCAGCTCCCTGAAGGTGTGGACCA
  GTCACATGAAGAGGACCATCCAGACAGCTGAGGCCCTGGGTGTCCCCCATGAGCAGTGGAAGGCCCTGAA
  TGAGATTGATGCGGGTGTCTGTGAGGAGATGACCTATGAAGAAATCCAGGAACATTACCCTGAAGAATTT
  GCACTGCGAGACCAAGATAAATATCGCTACCGCTATCCCAAGGGAGAGTCCTATGAGGATCTGGTTCAGC
  GTCTGGAGCCAGTGATAATGGAGCTAGAACGACAGGAGAATGTACTGGTGATCTGCCACCAGGCTGTCAT
  GCGGTGCCTCCTGGCCTATTTCCTGGATAAAAGTTCAGATGAGCTTCCATATCTCAAGTGCCCTCTGCAC
  ACAGTGCTCAAACTCACTCCTGTGGCTTATGGCTGCAAAGTGGAATCCATCTACCTGAATGTGGAGGCCG
  TGAACACACACCGGGAGAAGCCTGAGAATGTGGACATCACCCGGGAACCTGAGGAAGCCCTGGATACTGT
  CCCAGCCCACTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG224599 representing NM_002625
  Red=Cloning site Green=Tags(s)
  
  MSPEMGELTQTRLQKIWIPHSSGSSRLQRRRGSSIPQFTNSPTMVIMVGLPARGKTYISTKLTRYLNWIG
  TPTKVFNLGQYRREAVSYKNYEFFLPDNMEALQIRKQCALAALKDVHNYLSHEEGHVAVFDATNTTRERR
  SLILQFAKEHGYKVFFIESICNDPGIIAENIRQVKLGSPDYIDCDREKVLEDFLKRIECYEVNYQPLDEE
  LDSHLSYIKIFDVGTRYMVNRVQDHIQSRTVYYLMNIHVTPRSIYLCRHGESELNIRGRIGGDSGLSVRG
  KQYAYALANFIQSQGISSLKVWTSHMKRTIQTAEALGVPHEQWKALNEIDAGVCEEMTYEEIQEHYPEEF
  ALRDQDKYRYRYPKGESYEDLVQRLEPVIMELERQENVLVICHQAVMRCLLAYFLDKSSDELPYLKCPLH
  TVLKLTPVAYGCKVESIYLNVEAVNTHREKPENVDITREPEEALDTVPAHY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002625
• ORF Size: 1413 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002625.1, NP_002616.1
• RefSeq Size: 1741 bp
• RefSeq ORF: 1416 bp
• Locus ID: 5207
• Cytogenetics: Xp11.21
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism
• Gene Summary: 'This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]'