PFKFB1 (NM_002625) Human Untagged Clone

CAT#: SC303230

PFKFB1 (untagged)-Human 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 (PFKFB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PFKFB1
• Synonyms: F6PK; HL2K; PFRX
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002625 edited
  GGACAGGTAGTAAGATAGGAAGTGAGGCCAGGTACCTTGTGGGCAGTGATGTCATTCGGT
  GCGACTCCTAAGATGTCTCCAGAGATGGGAGAGCTCACCCAAACCAGGTTGCAGAAGATC
  TGGATTCCACACAGCAGCGGCAGCAGCAGGCTGCAACGGAGAAGGGGCTCATCCATACCC
  CAGTTTACCAATTCCCCCACAATGGTGATCATGGTGGGTTTACCAGCTCGAGGCAAGACC
  TATATCTCCACAAAGCTCACACGATATCTCAACTGGATAGGAACACCAACTAAAGTGTTT
  AATTTAGGCCAGTATCGACGAGAGGCAGTGAGCTACAAGAACTATGAATTCTTTCTTCCA
  GACAACATGGAAGCCCTGCAAATCAGGAAGCAGTGCGCCCTGGCAGCCCTGAAGGATGTT
  CACAACTATCTCAGCCATGAGGAAGGTCATGTTGCGGTTTTTGATGCCACCAACACTACC
  AGAGAACGACGGTCACTGATCCTGCAGTTTGCAAAAGAACATGGTTACAAGGTGTTTTTC
  ATTGAGTCCATTTGTAATGACCCTGGCATAATTGCAGAAAACATCAGGCAAGTGAAACTT
  GGCAGCCCTGATTATATAGACTGTGACCGGGAAAAGGTTCTGGAAGACTTTCTAAAGAGA
  ATTGAGTGCTATGAGGTCAACTACCAACCCTTGGATGAGGAACTGGACAGCCACCTGTCC
  TACATCAAGATCTTCGACGTGGGCACACGCTACATGGTGAACCGAGTGCAGGATCACATC
  CAGAGCCGCACAGTCTACTACCTCATGAATATCCATGTCACACCTCGCTCCATCTACCTT
  TGCCGACATGGCGAGAGTGAACTCAACATCAGAGGCCGCATCGGAGGTGACTCTGGCCTC
  TCAGTTCGCGGCAAGCAGTATGCCTATGCCCTGGCCAACTTCATTCAGTCCCAGGGCATC
  AGCTCCCTGAAGGTGTGGACCAGTCACATGAAGAGGACCATCCAGACAGCTGAGGCCCTG
  GGTGTCCCCTATGAGCAGTGGAAGGCCCTGAATGAGATTGATGCGGGTGTCTGTGAGGAG
  ATGACCTATGAAGAAATCCAGGAACATTACCCTGAAGAATTTGCACTGCGAGACCAAGAT
  AAATATCGCTACCGCTATCCCAAGGGAGAGTCCTATGAGGATCTGGTTCAGCGTCTGGAG
  CCAGTGATAATGGAGCTAGAACGACAGGAGAATGTACTGGTGATCTGCCACCAGGCTGTC
  ATGCGGTGCCTCCTGGCCTATTTCCTGGATAAAAGTTCAGATGAGCTTCCATATCTCAAG
  TGCCCTCTGCACACAGTGCTCAAACTCACTCCTGTGGCTTATGGCTGCAAAGTGGAATCC
  ATCTACCTGAATGTGGAGGCCGTGAACACACACCGGGAGAAGCCTGAGAATGTGGACATC
  ACCCGGGAACCTGAGGAAGCCCTGGATACTGTCCCAGCCCACTACTGAGCCCTTTCCAAG
  AAGTCAAACTGCCTGTGTCCTCATCGCCTTCCACCTTTAGGAAATGCTATCTTTGCTCTT
  CTCCTACTCTGCCTTGGCCTCACTGAGGCACCCCACTTCCAGTGAAGAAGTCCTCCGCAA
  CTCCCAAACAAGCCTCG
  
• Restriction Sites: Please inquire
• ACCN: NM_002625
• Insert Size: 1600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared to NM_002625.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002625.1, NP_002616.1
• RefSeq Size: 1741 bp
• RefSeq ORF: 1416 bp
• Locus ID: 5207
• Cytogenetics: Xp11.21
• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism
• Gene Summary: 'This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]'
  Transcript Variant: This variant (1) encodes the longest isoform (1).